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United States Department of Agriculture

Agricultural Research Service

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Louela Castrillo
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Louela A. Castrillo

Phone: (607) 255-7008
Fax: 607 255 1132

Research Scientist, Department of Entomology, Cornell University

Ph.D., North Carolina State University (Entomology/Genetics), 1997
M.S., North Carolina State University (Entomology/Microbiology), 1993

Research Projects

Microbial control of ambrosia beetles and their fungal symbionts. 

  1. Identify and assess genetic diversity of Ambrosiella spp. associated with ambrosia beetles Xylosandrus crassiusculus and X. germanus in the eastern US. Fungal symbionts will be identified using lsu and ssu ribosomal DNA sequences, and intraspecific diversity determined using ITS sequences and microsatilite markers.
  2. Determine susceptibility of ambrosia fungi to fungal antagonists and to entomopathogenic fungi Beauveria bassiana and Metarhizium brunneum.
  3. Determine impact of entomopathogenic fungi on survival of treated foundresses and brood development in galleries.

Selected Publications

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D. 2013.  Granulate ambrosia beetle, Xylosandrus crassiusculus (Coleoptera: Curculionidae), survival and brood production following exposure to entomopathogenic and mycoparasitic fungi. Biological Control 67, 220-226

Vandenberg, J. D., Griggs, M. H., Liu, H. P., Bauer, L. S., and Castrillo, L. A. 2013. Evaluation of imidacloprid and Beauveria bassiana strain GHA used against the emerald ash borer, Agrilus planipennis, infesting green and white ash in a commercial nursery. Journal of Invertebrate Pathology (submitted)

Hajek, A. E., Nielsen, C., Kepler, R. M., Long, S., and Castrillo, L. A.  2013.  Fidelity among Sirex woodwasps and their fungal symbionts.  Microbial Ecology 65, 753-762.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2012.  Brood production by ambrosia beetle Xylosandrus germanus (Coleoptera: Curculionidae) and growth of its fungal symbiont on artificial diet made with sawdust from different tree species. Environmental Entomology 41, 822-827.

Gibson, D. M., Castrillo, L. A., Donzelli, B. G. G., and Milbrath, L. R. 2011.  First report of blight caused by Sclerotium rolfsii on the invasive weed, Vincetoxicum rossicum (pale swallow-wort) in western New York.  Plant Disease (DOI: 10.1094/PDIS-08-11-0692)

Castrillo, L. A., Griggs, M. H., Ranger, C. M., Reding. M. E., and Vandenberg, J. D.  2011.  Virulence of commercial strains of Beauveria bassiana and Metarhizium brunneum (Ascomycota: Hypocreales) against adult Xylosandrus germanus (Coleoptera: Scolytidae) and impact on brood.  Biological Control (in press)

Castrillo, L. A. Liu, H.-P., Bauer, L. S., Griggs, M. H., and Vandenberg, J. D.  2010. Beauveria bassiana (Ascomycota: Hypocreales) isolates associated with Agrilus planipennis (Coleoptera: Buprestidae) populations in Michigan. Biological Control 54, 135-140.

Castrillo, L. A., Griggs, M. H., Liu, H.-P., Bauer, L. S., and Vandenberg, J. D.  2010.  Assessing deposition and persistence of Beauveria bassiana GHA (Ascomycota: Hypocreales) applied for control of the emerald ash borer, Agrilus planipennis (Coleoptera: Buprestidae), in a commercial nursery.  Biological Control 54, 61-67.

Braun, S. E., Castrillo, L. A., Sanderson, J. P., Daughtrey, M. L., and Wraight, S. P. 2010. Transstadial transmission of Pythium in Bradysia impatiens and lack of larval vectoring capacity. Phytopathology 100, 1307-1313.

Castrillo, L. A. and Humber, R. A.  2009.  Identification and Diagnosis of Fungi.  In Insect Pathogens. Molecular Approaches and Techniques (Stock, S. P., Vandenberg, J. D., Glazer, I.,  and  Boemare, N., Eds).  CABI Publishing, Wallingford, UK.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D.  2008.  Quantitative field detection of Beauveria bassiana GHA (Ascomycota: Hypocreales), a potential microbial control agent  of the emerald ash borer, by real-time PCR. Biological Control 45, 163-169.

Castrillo, L. A., Ugine, T. A., Filotas, M. J., Sanderson, J., Vandenberg, J. D., and Wraight, S. P.  2008.  Molecular characterization and comparative virulence of Beauveria bassiana isolates (Ascomycota: Hypocreales) associated with the greenhouse shore fly, Scatella tenuicosta (Diptera: Ephydridae).  Biological Control 45, 154-162.

Castrillo, L. A., Thomsen, L., Juneja, P. and Hajek, A. E. 2007.  Detection and quantification of Entomophaga maimaigaresting spores in forest soil using real-time PCR.  Mycological Research 111, 324-331.

Castrillo, L. A., Vandenberg, J. D., and Roberts, D. W. 2005. The fungal past, present and future: Germination, ramification and reproduction. Journal of Invertebrate Pathology 89, 46-56.

Filotas, M. J., Castrillo, L. A., Vandenberg, J. D., Sanderson, J., and Wraight, S. P. 2005. Novel isolates of the entomopathogenic fungus Beauveria bassianafor biological control of the shore fly, Scatella tenuicosta. IOBC /WPRS Bull. 28, 95-98.

Castrillo, L. A., Griggs, M. H., and Vandenberg, J. D. 2004. Vegetative compatibility groups in indigenous and mass-released strains of the entomopathogenic fungus Beauveria bassiana: likelihood of recombination in the field. Journal of Invertebrate Pathology 86, 26-37.

Castrillo, L. A., Vandenberg, J. D., and Wraight, S. P. 2003. Strain-specific detection of introduced Beauveria bassianain agricultural fields by use of sequence-characterized amplified region markers. Journal of Invertebrate Pathology 82, 75-83.

Castrillo, L. A., Rutherford, S. T., Lee, R. E., and Lee, M. R. 2001. Enhancement of ice-nucleating activity in Pseudomonas fluorescensand its effect on efficacy against Colorado potato beetles. Biological Control 21, 27-34.

Castrillo, L. A., Lee, R. E., Wyman, J. A., Lee, M. R. and Rutherford, S. T. 2001. Field persistence of ice-nucleating Pseudomonas fluorescensin overwintering Colorado potato beetles. Biological Control 21, 11- 18.

Humphreys, T. L., Castrillo, L. A., Lee, M. R. 2001. Sensitivity of partially purified ice nucleation activity of Fusarium avenaciumSRSF 616. Current Microbiology 42, 330-338.

Lee, R. E., Castrillo, L. A., Lee, M. R., Wyman, J. A., and Costanzo, J. P. 2001. Using Ice nucleating bacteria to reduce winter survival of Colorado potato beetles: Development of a novel strategy for biological control. In Insect Timing: Circadian Rhythmicity and Seasonality (D. L. Denlinger, J. M. Giebultowicz, and D. S. Saunders, Eds.) pp. 213- 227. Elsevier, New York.

Castrillo, L. A., Lee, R. E., Lee, M. R. and Rutherford, S. T. 2000. Identification of ice-nucleating active bacteria for biological control of overwintering Colorado potato beetles. Journal of Economic Entomology 93, 226-233.

Castrillo, L. A., Lee, R. E., Lee, M. R., and Wyman, J. A. 2000. Long-term retention of the ice nucleating Pseudomonas fluorescensby overwintering Colorado potato beetles. Cryo-Letters 21, 5-12.

Castrillo, L. A., Wiegmann, B. M., and Brooks, W. M. 1999. Genetic variation in Beauveria bassianapopulations associated with the darkling beetle, Alphitobius diaperinus, in poultry houses. Journal of Invertebrate Pathology 73, 269-275.

Castrillo, L. A. and Brooks, W. M. 1998. Differentiation of Beauveria bassianaisolates from the darkling beetle, Alphitobius diaperinus, using isozyme and RAPD analyses. Journal of Invertebrate Pathology 72, 190-196.

Last Modified: 11/13/2013
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