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United States Department of Agriculture

Agricultural Research Service

Biochemistry
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Objectives

  • Determine optimum duration of reduced irrigation without significant yield loss
  • Compare plant response to different drought tolerance/avoidance strategies
  • Determine biochemical responses(s) associated with specific plant physiological response(s)
  • Determine gene expression patterns in specific biochemical pathways or proteins and correlate with plants’ performance and yield
  • Incorporate knowledge of specific biochemical and gene expression patterns to develop molecular markers for the development of drought tolerant/resistant peanut varieties

 50 day Control with 100% and 50% irrigation, respectively

 50 day Control with 100% irrigation

 50 day Control with 50% irrigation

 Early season water deficit (up to 50 days after germination) provides some drought tolerant benefits.


Biochemical and Gene Expression Study of Peanut TSWV Resistance

Objectives

  • Determine the levels of Tomato Spotted Wilt Virus infection using molecular diagnosis
  • Correlate peanut TSWV symptoms to the number of viral particles
  • Determine biochemical and gene expression patterns of plant responses to the degrees of viral infection
  • Correlate specific peanut proteins and genes to the degrees of viral resistance
  • Correlate viral resistance in different peanut varieties and determine specific peanut proteins and gene expression patterns
  • Incorporate understanding of specific biochemical and gene expression patterns to develop molecular markers for TSWV tolerance/resistance in peanut

 TSWV infected peanut and PCR diagnosis

 Tomato Spotted Wilt Virus (TSWV) infected peanut and PCR diagnosis


Application of Genomics to Understand Peanut Drought Response

  • Utilize peanut micro-arrays to determine gene expression patterns in drought resistant versus drought susceptible peanut varieties
  • Determine specific regulated proteins or genes that may be involved in drought tolerance
  • Develop gene expression profiles on specific proteins or genes using controlled experiments
  • Incorporate information on newly discovered proteins or genes to develop moleculamarkers for drought tolerance/resistance in peanut
     

     Genomic application involves gene chip micr-array, molecular marker development and variety genetic analysis

    Genomic application involves gene chip micro-array, molecular marker development and variety genetic analysis

 

For more information contact Phat Dang.


Last Modified: 1/22/2009