Plant Cell Reports (1998) 17: 726-730

J. M. Myers - P. W. Simon

Continuous callus production and regeneration of garlic (Allium sativum L.) using root segments from shoot tip-derived plantlets

Received: 14 October 1997 / Revision received: 26 December 1997 / Accepted: 12 January 1998

Abstract  Root segments from shoot tip-derived plantlets of the garlic (Allium sativum L.) clones 'DDR7099', 'PI383819', and 'Piacenza' were utilized as an explant source for continuous, friable callus production. The best callus production occurred on root segments initally cultured on medium with 4,5 M 2,4-dichlorophenoxyacetic acid (2,4-D) for 8 weeks, then subcultured to medium with 4.7 M 4-amino-3,5,6-trichloropicolinic acid (picloram) +0.49 M 6-(-( -dimethylallylamino)purine (2iP) for 8 weeks. Embryogenic, friable callus was transferred to liquid medium for 1 month and then transferred to solid regeneration medium for 14 weeks. The best shoot and root regeneration (85.3% and 35.8%, respectively) occurred on 4-month-old calli from the clone 'DDR7099'. In all clones, regeneration rate decreased as callus age increased.

Key words  Garlic ^. Allium sativum L. ^. Root segments ^. Regeneration ^. Picloram

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J. M. Myers
Department of Horticulture, 1575 Linden Drive,
University of Wisconsin, Madison, WI 53706, USA

P. W. Simon
USDA Vegetable Crops Research,
Department of Horticulture, 1575 Linden Drive,
University of Wisconsin, Madison, WI 53706, USA
Fax: (608) 262-4743
E-mail: psimon@facstaff.wisc.edu

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