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United States Department of Agriculture

Agricultural Research Service

Volunteer Project Listing
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1.      Transgenic plants

2.      Mature tissue transformation

3.      Citrus genomics

4.      General computer work

5.      Plant tissue culture I

6.      Plant tissue culture II


 

If any of these positions interest you, then contact me and we will arrange a time for you to visit the lab and learn more.  If you are still interested, then Volunteering in a Federal Laboratory explains what paperwork is required.


Title:

Transgenic plants

Topic areas:

Plant breeding, genetics, genetic engineering, biochemistry

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

A population of ~80 citrus trees that are transgenic for AP24 (anti-Phytophthora protein 24) must be tested for GUS enzyme activity.  Project requires 1) collecting leaves, 2) crushing leaves in a buffer solution, 3) preparing leaf extract for enzyme analysis, and 4) measuring GUS enzyme activity by fluorometry.

Skills required:

Some basic biochemistry and lab techniques would be helpful.  However, the assay can be taught to someone who is organized, detail-oriented, and careful.

Hours/week:

4


Title:

Mature tissue transformation

Topic areas:

Horticulture, plant breeding

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

Labeling, light pruning, and organizing greenhouse citrus plants for use in genetic engineering experiments.

Skills required:

Basic horticultural plant care.

Hours/week:

1


Title:

Citrus genomics

Topic areas:

Plant breeding, genetics, biochemistry, plant cell culture

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

Develop procedure to isolate pollen protoplasts, which will be used for genomic DNA sequencing.

Skills required:

Some basic biochemistry, plant biology, and lab techniques would be helpful.  However, the various steps can be taught to someone who is organized, detail-oriented, and careful.

Hours/week:

2-4


Title:

General computer work

Topic areas:

Word processing, spreadsheets, and document/picture scanning.

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

Data entry into spreadsheets.

Skills required:

Basic computer skills and familiarity with the use of spreadsheets.

Hours/week:

2


Title:

Plant tissue culture I

Topic areas:

Plant tissue culture, horticulture

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

Basic plant tissue culture experiments.

Skills required:

Basic lab techniques would be helpful.  However, the various steps can be taught to someone who is organized, detail-oriented, and careful.

Hours/week:

2-4


Title:

Plant tissue culture II

Topic areas:

Plant tissue culture, horticulture

Where:

Ft. Pierce, FL

Location:

U.S. Horticultural Research Laboratory

Contact:

Dr. Randall P. Niedz, (772) 462-5800

Description:

General assistance in plant tissue culture laboratory to include preparation of plant material for in vitro culture, cleaning of glassware, preparation of culture media, plant tissue culture

Skills required:

Basic lab techniques and plant tissue culture would be helpful.  However, the various steps can be taught to someone who is organized, detail-oriented, and careful.

Hours/week:

2-4


 

 

 


Last Modified: 8/21/2014
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