CURRICULUM VITAE - James J. Giovannoni
Robert W. Holley Research Center for Agriculture and Health
Cornell University Campus
Ithaca, NY 14850
Phone: (607) 847-8531
FAX: (607) 254-1502
1985 - 1990 Ph.D. University of California, Berkeley
Molecular and Physiological Plant Biology
1981 - 1985 BS University of California, Davis, Biochemistry (minor in Philosophy)
2000 - Research Molecular Biologist, ARS/BTI, Ithaca NY (10/00 - present)
1999 - 2000 Director, Center for Nutrition, Health and Food Genomics, Texas A&M
1997 - 2000 Associate Professor, Department of Horticultural Sciences, Texas A&M
1992 - 1997 Assistant Professor, Department of Horticultural Sciences, Texas A&M
1991 - 1992 Post-doctoral Research Associate, NSF/DOE/USDA Plant Science Center, Cornell University
1990 - 1991 Post-doctoral Research Associate, Department of Plant Breeding and Biometry, Cornell University
1985 - 1990 Graduate Research Assistant, University of California, Berkeley
The focus of research in our laboratory is molecular and genetic analysis of fruit ripening and related signal transduction systems. We are investigating the regulation of ripening using tomato as a model system. Experimental approaches include 1) positional cloning of loci known via mutation to harbor genes necessary for normal fruit development and ripening, 2) isolation of candidate ripening regulatory genes based on expression pattern or relationship to ripening-related signal transduction systems (egs. ethylene, light), and functional analysis in transgenic plants, and 3) development and utilization of tools for functional genomics with current targets of exploration including genome sequencing and genome-wide expression profiling during ripening and as related to fruit quality and nutritional composition.
CURRENT FACULTY AFFILIATIONS
Boyce Thompson Institute for Plant Research, Research Scientist (10/00 - present)
Department of Plant Biology, Cornell University, Adjunct Professor (5/01 - present)
Department of Horticulture, Cornell University, Adjunct Professor (11/01 - present)
Department of Plant Breeding, Cornell University, Adjunct Professor (5/03 - present)
Department of Horticulture, Texas A&M, Adjunct Professor (10/00 - present)
TEACHING (Texas A&M)
HORT/PPHY/GENE 411 Biotechnology for Crop Improvement S97, S98, S99, S00
HORT or GENE or PPHY 485 Independent Study - total of 11 students
HORT/PPHY 630 Post-harvest Biology/Physiology/Genetics. F93, F94, F95, F97
HORT 681 Graduate Seminar S96
HORT 685 Independent Study (graduate students from other
labs interested in learning molecular mapping
who spend a semester in the lab) - total of 6 students
BIOPL 742 (Cornell) F01
BIOPL 653.1 (Cornell) F02, F03, F04
Thesis Committees Chaired:
Program Degree Completed Current
GENE M.Sc. 1 0
Ph.D. 3 0
PPHY M.Sc. 1 0
Ph.D. 3 0
HORT M.Sc. 2 0
Ph.D. 1 0
Plant Biology Ph.D 1 1 (Plant Biology)
McKnight Foundation Fellowship. August 1985 - December 1986 (graduate student).
Executive Committee for the Biotechnology Research and Education Program Fellowship.
January 1988 - April 1990 (graduate student).
Executive Committee for the Biotechnology Research and Education Program Fellowship. April
1990 - August 1990 (post-doctoral).
NSF/DOE/USDA Plant Science Center Postdoctoral Fellow. Cornell University. September 1,
1991 -September 24, 1992.
Margossian, L., Federman, A., Giovannoni, J., and Fischer, R. (1988) Ethylene-regulated expression of a tomato fruit ripening gene encoding a proteinase inhibitor 1 with a glutamic residue at the reactive site. PNAS USA. 85:8012-8016.
Giovannoni, J., DellaPenna, D., Bennett, A., and Fischer, R. (1989) Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor)tomato fruit results in polyuronide degradation but not fruit softening. The Plant Cell. 1:53-63.
DellaPenna, D., Lashbrook, C., Toenjes, K., Giovannoni, J., Fischer, R., and Bennett, A. (1990). Polygalacturonase isozymes and pectin depolymerization in transgenic rin tomato fruit. Plant Physiol. 94:1882-1886.
Giovannoni, J., Wing, R., Ganal, M. and Tanksley, S. (1991). Isolation of molecular markers from specific genomic regions through the use of isogenic DNA pools and existing mapping populations. Nuc. Acids Res. 19:6553-6558.
Penarrubia, L., Kim, R., Giovannoni, J., Kim, S. and Fischer, R. (1992) Production of the sweet protein monellin in transgenic plants. Bio/Technology. 10:561-563.
Tanksley, S., Ganal, M., Prince, J., deVincente, C., Bonierbale, M., Broun, P., Fulton, T., Giovannoni, J., Grandillo, S., Martin, G., Messeguer, R., Miller, J., Miller, L., Paterson, A., Pineda, O., Roder, M., Wing, R., Wu, W., and Young, N. (1992) High density molecular maps of the tomato and potato genomes. Genetics. 132:1141-1160.
Churchill, G., Giovannoni, J. and Tanksley, S. (1993) Pooled-sampling makes high-resolution mapping practical with DNA markers. PNAS USA. 90:16-20.
Gray, J., Picton, S., Giovannoni, J. and Grierson, D. (1994) The use of transgenic and naturally occurring mutants to understand and manipulate tomato fruit ripening. Plant, Cell and Environment. 17:557-551.
Lanahan, M., Yen, H. Giovannoni, J. and H. Klee (1994) The Never-ripe mutation blocks ethylene perception in tomato. The Plant Cell. 6:521-530.
Oh, B. J., Balint, D. and Giovannoni, J. (1995) A modified procedure for PCR-based differential display and demonstration of use in plants for isolation of fruit ripening-related genes. Plant Molecular Biology Reporter 13(1): 70-81
Yen, H., Lee, S., Tanksley, S., Lanahan, M., Klee, H. and Giovannoni, J. (1995) The tomato Never-ripe locus regulates ethylene-inducible gene expression and is linked to a homologue of the Arabidopsis ETR1 gene. Plant Physiology 107:1343-1353
Giovannoni, J., Noensie, E., Ruezinsky, D., Lu, X., Tracy, S., Ganal, M., Martin, G., Pillen, K. and Tanksley, S. (1995) Molecular genetic analysis of the ripening-inhibitor and non-ripening loci of tomato: a first step in genetic map-based cloning of fruit ripening genes. Molecular and General Genetics 248(2): 195-206.
Lu, Xianghuai., Ruezinsky, D. and Giovannoni, J. (1995) Rapid isolation of terminal sequences from cloned plant DNA fragments. Plant Molecular Biology Rep. 13(4): 369-376.
Wilkinson, J., Lanahan, M., Yen, H., Giovannoni, J. and Klee, H. (1995) An ethylene-inducible component of signal transduction encoded by Never-ripe. Science 270:1807-1809.
Pillen, K., Alpert, K., Giovannoni, J., Ganal, M. and Tanksley, S. (1996) Rapid and reliable screening of a tomato YAC library exclusively based on PCR. Plant Molecular Biology Reporter 14(1): 58-67.
Yen, H., Shelton, A., Howard, L. Vrebalov, J. and Giovannoni, J. (1997) The tomato high pigment (hp) locus maps to chromosome 2 and influences plastome copy number and fruit quality. Theoretical and Applied Genetics 95:1069-1079
Alcala, J., Giovannoni, J., Pike, L., and Reddy, S. (1997) Application of Genetic Bit Analysis for allelic selection in plant breeding. Molecular Breeding 3:495-502
Lashbrook, C., Giovannoni, J., Hall, B., Fischer, R., and Bennett, A. (1998) Transgenic analysis of tomato endo-beta-1,4-glucanase gene function. Role of CEL1 in floral abscission. The Plant Journal 13:303-310.
Alcala, J., Pike, L., and Giovannoni, J. (1999) Marker-assisted selection for cytoplasmic male sterility in onion and identification of plastome variants useful for cultivar identification and pedigree analysis. J. Amer. Soc. Hort. Sci. 124:122-127
Giovannoni, J., Yen, H., Shelton, B., Miller, S., Kannan, P., Vrebalov, J., Tieman, D., Hackett, R., Grierson, D., and Klee, H. (1999) Genetic mapping of ripening and ethylene-related loci in tomato. Theoretical and Applied Genetics 98:1005-1013
Thompson, A., Barry, C., Jarvis, M., Vrebalov, J., Giovannoni, J., Grierson, D., and Seymour, G. (1999) Molecular and genetic characterization of a novel pleiotropic tomato-ripening mutant. Plant Physiology 120: 383-389.
Casselman, A., Vrebalov, J. Conner, J., Singhal, A., Giovannoni, J., Nasrallah, M., Nasrallah, J. (2000) Recombinational analysis of the Brassica S locus. The Plant Cell 12: 23 - 33.
Giovannoni, J. (2001) Molecular Regulation of Fruit Ripening. Annual Review of Plant Physiology and Plant Molecular Biology. 52:725-749.
Barry, C., Fox, E., Yen, H., Lee, S., Ying, T., Grierson, D. and Giovannoni J. (2001) Analysis of the ethylene response in the epinsastic (epi) mutant of tomato. Plant Physiology 127:58-66.
Giovannoni J, Payton P, Moore S, White R, Vrebalov J. (2002) Genetic Control of Fruit Quality and Prospects for Nutrient Modification. HortScience. 37: 453-456
Vrebalov J, Ruezinsky D, Padmanabhan V, White R, Medrano D, DrakeR , Schuch W, Giovannoni J. (2002) A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus. Science. 296:343-346
Van der Hoeven R, Ronning C, Giovannoni J, Martin G, Tanksley S. (2002) Deductions about the number, organization and evolution of genes in the tomato genome based on analysis of large expressed sequence tag collection and selective genomic sequencing. The Plant Cell, 14: 1441-1456
Moore S, Vrebalov J, Payton P, Giovannoni J. Use of genomics tools to isolate key ripening genes and analyze fruit maturation in tomato. (2002) J. Exp. Botany. 53: 2023-2030.
Leclercq, J., Adams-Phillips, L., Zegzouti, H., Jones, B., Latche, A., Giovannoni, J., Peche, J., and Bouzayen, M. (2002) LeCTR1, a tomato CTR1-like gene, demonstrates ethylene signaling ability in Arabidopsis and novel expression patterns in tomato. Plant Physiology. 130:1132-1142
Ruperti, B., Bonghi, C., Ziliotto, F., Pagni, S., Rasori, A., Varotto, S., Tunutti, P., Giovannoni, J., and Ramina, A. (2002) Characterization of a major latex protein (MLP) gene down-regulated by ethylene during peach fruitlet abscission. Plant Science. 163:265-272
Fei Z, Tang X, Alba R, Payton P, Giovannoni J (2003). Tomato Expression Database (TED) – An Interactive Management Tool for Tomato Expression Profiling Data. Proc Comp Sys Bioinform 03:424–425.
Basset G, Quinlivan E, Ravanel S, Rébeillé F, Nichols B, Shinozaki K, Seki M, Adams-Phillips L, Giovannoni J, Gregory J and Hanson A. (2004) Folate synthesis in plants: The p-aminobenzoate branch is initiated by a bifunctional PabA-PabB protein that is targeted to plastids. PNAS USA. 101:1496-1501
Giovannoni J. (2004) Genetic regulation of fruit development and ripening. The Plant Cell. 16: S170 -180.
Liu, Y., Roof, S., Ye, Z., Barry, C., van Tuinen, A., Vrebalov, J., Bowler, C., and Giovannoni, J. (2004) Manipulation of light signal transduction as a means of modifying fruit nutritional quality in tomato. PNAS USA. 26: 9897-9902
Adams-Phillips, L., Barry, C. and Giovannoni, J. (2004) Signal transduction systems regulating fruit ripening. Trends in Plant Science. 9: 331-338
Adams-Phillips, L., Barry, C., Kannan, P., Leclercq, J., Bouzayen, M., and Giovannoni, J. (2004) Evidence that CTR1-mediated ethylene signal transduction in tomato is encoded by a multigene family whose members display distinct regulatory features. Plant Molec. Biol. 54:387-404
Alba, R., Fei, Z., Payton, P., Liu, Y., Moore, S., Debbie, P., Gordon, J., Rose, J., Martin, G., Tanksley, S., Bouzayen, M., Jahn, M., and Giovannoni, J. (2004) ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development. Plant Journal. 39:697-714.
Rose, J., Bashir, S., Giovannoni, J., Jahn, M., and Saravanan, S. (2004) Tackling the plant proteome: Practical approaches, hurdles and experimental tools. Plant Journal. 39:715-733..
Fei, Z., Tang, X., Alba, R., White J., Ronning, C., Martin, G., Tanksley, S. and Giovannoni, J. (2004) Comprehensive EST analysis of tomato and comparative genomics of fruit ripening. Plant Journal. 40:47-59.
Basset, G., Ravanel, S., Quinlivan, E., White, R., Giovannoni, J., Rébeillé, F., Nichols, B., Shinozaki, K., Gregory, J. and Hanson, A. (2004) Folate synthesis in plants: the last step of the p-aminobenzoate branch is catalyzed by a plastidial aminodeoxychorismate lyase. Plant Journal. 40:453-461.
Barry, C., McQuinn, R., Thompson, A., Seymour, G., Grierson, D. and Giovannoni, J. (2005) Ethylene insensitivity conferred by the Green-ripe (Gr) and Never-ripe 2 (Nr-2) ripening mutants of tomato. Plant Physiology. 138:267-275.
Li, C., Liu, G., Schilmiller, A., Lee, G., Jayanty, S., Sageman, C., Vrebalov, J., Giovannoni, J. and Howe, G. (2005) Role of -Oxidation in Jasmonate Biosynthesis and Systemic Wound Signaling in Tomato. Plant Cell. 17:971-986.
Mueller LA, Tanksley SD, Giovannoni JJ, Van Eck J, Stack S, Choi D, Kim BD, Chen M, Cheng Z, Li C, Ling H, Xue Y, Seymour G, Bishop G, Bryan G, Sharma R, Khurana J, Tyagi A, Chattopadhyay, D, Singh NK, Stiekema W, Lindhout P, Jesse T, Lankhorst RK, Bouzayen M, Shibata D, Tabata S, Granell A, Botella MA, Giuliano G, Frusciante L, Causse M, and Zamir D (2005) The Tomato Sequencing Project, the first cornerstone of the International Solanaceae Project (SOL). Comp Funct Genom, 6:153-158.
Giovannoni, J.J. and El-Rakshy, S. 2005. Genetic Regulation of tomato fruit ripening and development and implementation of associated genomics tools. Acta Hort. 682:63-72
Ponce-Valadez, M., Watkins, C.B., Moore, S. and Giovannoni, J.J. 2005. Differential gene expression analysis of strawberry cultivar responses to elevated CO2 contrations during storage using a tomato cDNA microarray. Acta Hort. 682:255-262
Moore, S., Payton, P., Wright, M., Tanksley, S., and Giovannoni, J. (2005) Utilization of tomato microarrays for comparative gene expression analysis in the Solanaceae. J. Ex. Botany. In press.
Alba, R., Payton, P., Fei, Z., McQuinn, R., Debbie, P., Martin, G., Tanksley, S. and Giovannoni, J. Transcriptome and selected fruit metabolite analysis reveal multiple points of ethylene regulatory control during tomato fruit development. Plant Cell In press
REVIEWS and CHAPTERS
Bennett, A., DellaPenna, D., Fischer, R., Giovannoni, J., and Lincoln J. (1989) Tomato Fruit Polygalacturonase: Gene Regulation and Enzyme Function. In Biotechnology and Food Quality. (Shain-Dow Kung, ed.) University of Maryland Press. p167-180.
Giovannoni, J., DellaPenna, D., Lashbrook, C., Bennett, A., and Fischer, R. (1990) Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor) tomato fruit. In Plant Gene Transfer (Lamb, C. and Beachy, R. eds.) New York: Alan R. Liss, Inc. p81-100.
Giovannoni, J., DellaPenna, D., Lashbrook, C., Bennett, A., and Fischer, R. (1990) Expression of a chimeric polygalacturonase gene in transgenic rin tomato fruit. In Horticultural Biotechnology (Bennett, A. ed.) Alan R. Liss, Inc.
Giovannoni, J., DellaPenna, D., Bennett, A., and Fischer, R. (1991) Polygalacturonase and Tomato Fruit Ripening. Horticultural Reviews. Vol. 13 p67-103.
DellaPenna, D. and Giovannoni, J. (1991) Regulation of gene expression in ripening tomatoes. In Plant Biotechnology Series, Volume 2: Factors Governing Plant Gene Expression. (Grierson, D. ed.) Glasgow, Scotland: Blackie and Son Limited. pp. 182-216.
Giovannoni, J. (1993) Molecular biology of fruit development and ripening. In Methods in Plant Molecular Biology. (Bryant, J. ed.) Academic Press. Vol. 10: 253-287.
Giovannoni, J and Yen, H. (1997) Ethylene Signal Transduction. Trans. Malaysian Soc. Plant Physiol. 6:1-8.
Giovannoni, J. Molecular genetic analysis of ethylene-regulated and developmental components of tomato fruit ripening. (1997) In Biology and Biotechnology of the Plant Hormone Ethylene. (Kanellis, A. ed.). Kluwer Academic Publishers. p133-140.
Giovannoni, J., Kannan, P., Lee, S., and Yen, H. (1998) Genetic approaches to manipulation of fruit development and quality in tomato. In Genetic and Environmental Manipulation of Horticultural Crops. (Cockshull, K. ed.) CAB International Publishers. p 1-15.
Giovannoni, J., Fox, E., Kannan, P., Lee, S., Padmanabhan, V., and Vrebalov, J. (1999) Analysis of gene expression and mutants influencing ethylene responses and fruit development in tomato. In Biology and Biotechnology of the Plant Hormone Ethylene II. (Kanellis, A. ed.) Kluwer Academic Publishers. p 119-127.
Moore, S., Payton, P. and Giovannoni, J. (2002) DNA microarrays for gene expression analysis. In: Practical Approach: Molecular Plant Biology Vol. 2. (P. Gilmartin and C. Bowler, Eds) Oxford University Press. p65 - 76.
McCaskill A R, and Giovannoni J. (2002) Use of molecular markers for fruit crop improvement. In: Molecular Techniques in Crop Improvement (S.Mohan Jain, DS Brar and BS Ahloowalia eds.) Kluwer Academic Publishers, The Netherlands, p 283-297.
Adams-Phillips, L., Alba, R., Barry, C., and Giovannoni, J. (2003) Genomics of ethylene signal transduction in tomato. In: Biology and Biotechnology of the Plant Hormone Ethylene (Vendrel, M. et al. eds.). IOS Press, Amsterdam. p 131-136.
Giovannoni J. (2004) Plant genetic modification: gene isolation. In: Handbook of Plant Biotechnology Vol 1. (P. Cristou, H. Klee Eds.) Wiley and Sons, Ltd. p 65-68
Fox E, Giovannoni J. Genetic control of fruit ripening. In: Genetic Improvement of Solanaceous Crops, Vol. 1: Tomato ( M.K. Razdan, A. Mattoo Eds.), Science Publishers Inc. In press
Endo-1,4-B-Glucanase Genes and Their Use in Plants. US Patent No. 5,168,064 issued December 1, 1992; Patent No. 5,328,999 issued July 12, 1994. Co-inventors: Bennett, A., Fischer, R., Giovannoni, J. and Lashbrook, C.
Constructs for expression of monellin in plant cells. US Patent No. 5,234,834 issued August 10, 1993. Co-inventors: Cho, J., Fischer, R., Giovannoni, J., Kim, R., Kim, S. and Penarrubia, D.
RIN gene for use in manipulation of fruit quality and ethylene response. US Patent No. 6,787,687 issued September 7, 2004. Co-Inventors: Giovannoni, J., Tanksley, S., Padmanabhan, V., Vrebalov, J., Ruezinsky, D., and White, R.
NOR gene for use in manipulation of fruit quality and ethylene response. US Patent No. 6,762,347 issued July 13, 2004. Co-Inventors: Giovannoni, J., Tanksley, V., Vrebalov, J., and Noensie, E.