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Agricultural Research Service United States Department of Agriculture
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David Johnston
Crop Conversion Science and Engineering
Food Technologist

Phone: (215) 836-3756
Fax: (215) 233-6406
Room 3125
600 E MERMAID LN
ERRC
WYNDMOOR, PA, 19038-8598

Projects
Enzyme-Based Technologies for Milling Grains and Producing Biobased Products and Fuels
Appropriated (D)
  Accession Number: 418467
From Barley to Biomass - the Development of a Regional Multi-Feedstock Biorefinery
Appropriated (D)
  Accession Number: 418394
Economic Competitiveness of Renewable Fuels Derived from Grains and Related Biomass
Appropriated (D)
  Accession Number: 409037

Publications
Importance of protein rich components in the emulsifying properties of corn fiber gum. - (Peer Reviewed Journal) - (18-Sep-09)
Production of astaxanthin from corn fiber as a value-added co-product of fuel ethanol fermentation - (Peer Reviewed Journal)
Nghiem, N.P., Montanti, J., Johnston, D. 2009. Production of astaxanthin from corn fiber as a value-added co-product of fuel ethanol fermentation. Applied Biochemistry and Biotechnology. 154:227-237.
Enzymatic corn wet milling: engineering process and cost model - (Peer Reviewed Journal)
Ramirez, E., Johnston, D., Mcaloon, A.J., Singh, V. 2009. Enzymatic corn wet milling: engineering process and cost model. Biotechnology for Biofuels. 2:2.
Corn fiber gum: New structure/function relationships for this potential beverage flavor stabilizer - (Review Article)
Yadav, M.P., Johnston, D., Hicks, K.B. 2009. Corn fiber gum: New structure/function relationships for this potential beverage flavor stabilizer. Food Hydrocolloids Journal. 23:1488-1493.
Fractionation, characterization and study of emulsifying properties of corn fiber gum - (Peer Reviewed Journal)
Yadav, M.P., Parris, N., Johnston, D., Hicks, K.B. 2008. Fractionation, characterization and study of emulsifying properties of corn fiber gum. Journal of Agriculture and Food Chemistry. 56, p.4181-4187.
Angiotensin I Converting enzyme inhibitory peptides from commercial wet- and dry-milled corn germ - (Peer Reviewed Journal)
Parris, N., Moreau, R.A., Johnston, D., Dickey, L.C., Rotimi, A.E. 2008. Angiotensin I Converting enzyme inhibitory peptides from commercial wet- and dry-milled corn germ. Journal of Agricultural and Food Chemistry. 56(8):2620-2623.
Enhancing water removal from whole stillage by enzyme addition during fermentation - (Peer Reviewed Journal)
Henriquez, A., Johnston, D., Al-Dahhan, M. 2008. Enhancing water removal from whole stillage by enzyme addition during fermentation. Cereal Chemistry. 85(5):685-688.
Fiber separation from distillers dried grains with solubles using a larger elutriation apparatus and use of fiber as a feedstock for corn fiber gum production - (Peer Reviewed Journal)
Srinivasan, R., Yadav, M.P., Belyea, R.L., Rausch, K.D., Pruiett, L.E., Johnston, D., Tumbleson, M.E., Singh, V. 2008. Fiber separation from distillers dried grains with solubles using a larger elutriation apparatus and use of fiber as a feedstock for corn fiber gum production. Biological Engineering 1(1):p.39-49.
A process for the aqueous enzymatic extraction of corn oil from dry-milled corn germ and enzymatic wet milled corn germ (E-Germ) - (Peer Reviewed Journal)
Moreau, R.A., Dickey, L.C., Johnston, D., Hicks, K.B. 2009. A process for the aqueous enzymatic extraction of corn oil from dry-milled corn germ and enzymatic wet milled corn germ (E-Germ). Journal of the American Oil Chemists' Society 86:p.469-474.
Engineering process and cost model for a conventional corn wet milling facility - (Peer Reviewed Journal)
Ramirez, E., Johnston, D., Mcaloon, A.J., Yee, W.C., Singh, V. 2008. Engineering process and cost model for a conventional corn wet milling facility. Industrial Crops and Products 27, p.91-97.
Characterization of corn fiber gums from coarse and fine fiber and a study of their emulsifying properties - (Peer Reviewed Journal)
Yadav, M.P., Johnston, D., Hicks, K.B. 2007. Characterization of corn fiber gums from coarse and fine fiber and a study of their emulsifying properties. Journal of Agriculture and Food Chemistry, 55,p.6366-6371.
Molecular characteristics of corn fiber gum and their influence on its emulsifying properties - (Peer Reviewed Journal)
Yadav, M.P., Fishman, M.L., Chau, H.K., Johnston, D., Hicks, K.B. 2007. Molecular characteristics of corn fiber gum and their influence on its emulsifying properties. Cereal Chemistry Vol. 84, No. 2, p.175-180.
Aqueous Enzymatic Oil Extraction: a "green" Bioprocess to Obtain Oil from Corn Germ and Other Oil-Rich Plant Materials - (Book/Chapter)
Moreau, R.A., Johnston, D., Dickey, L.C., Parris, N., Hicks, K.B. 2007. Aqueous enzymatic oil extraction: a "green" bioprocess to obtain oil from corn germ and other oil-rich plant materials, in "The industrial application of enzymes on carbohydrate based materials, ed by G. Eggleston and J. R. Vercellotti, American Oil Chemists' Society Press, pp 101-120. Book Chapter.
Comparison of raw starch hydrolyzing enzyme with conventional liquefaction and saccharification enzymes in dry-grind corn processing - (Peer Reviewed Journal)
Wang, P., Singh, V., Xue, H., Johnston, D., Rausch, K.D., Tumbleson, M.E. 2006. Comparison of raw starch hydrolyzing enzyme with conventional liquefaction and saccharification enzymes in dry-grind corn processing. Cereal Chemistry. 84(1):10-14.
Mycotoxin Control During Grain Processing. - (Proceedings/Symposium) - (09-Jul-06)
Corn Fiber: a Potential Gum Arabic Replacer for Beverage Flavor Emulsification - (Peer Reviewed Journal)
Yadav, M.P., Johnston, D., Hotchkiss, A.T., Hicks, K.B. 2007. Corn fiber: a potential gum arabic replacer for beverage flavor emulsification. Food Hydrocolloids 21, p.1022-1030.
Effect of B Vitamin and Lipid Supplementation to Improve Fermentation Characteristics of the Modified Dry Grind Process - (Proceedings/Symposium)
Ganti, M.S., Singh, V., Rausch, K., Johnston, D., Tumbleson, M.E. 2006. Effect of b vitamin and lipid supplementation to improve fermentation characteristics of the modified dry grind process. Proceedings of the American Society of Agricultural and Biological Engineers International (ASABE).Paper #066067.
Mathematical Modeling of Enzymatic Hydrolysis of Starch: Application to Fuel Ethanol Production - (Proceedings/Symposium)
Murthy, G.S., Singh, V., Rausch, K., Johnston, D., Tumbleson, M.E. Mathematical modeling of enzymatic hydrolysis of starch: application to fuel ethanol production. 2006. Proceedings of the American Society of Agricultural and Biological Engineers International (ASABE)Paper #066229.
Effects of ground corn particle size on ethanol yield and thin stillage soluble solids - (Peer Reviewed Journal)
Naidu, K., Singh, V., Johnston, D., Rausch, K.D., Tumbleson, M.E. 2006.Effects of ground corn particle size on ethanol yield and thin stillage soluble solids. Cereal Chemistry, Vol. 84, No. 1, p.6-9.
Enzymatic Corn Wet Milling: Results from a Commercial Plant Trial - (Proceedings/Symposium)
Johnston, D., Singh, V. 2007. Enzymatic corn wet milling: results from a commercial plant trial. Proceedings of the Corn Utilization and Technology Conference, June 5-7, 2006. Dallas, Texas.
Corn Wet Fractionation Prior to Fermentation for Recovery of Valuable Co-Products - (Proceedings/Symposium)
Hicks, K.B., Johnston, D., Moreau, R.A., Mcaloon, A.J., Ramirez, E.C., Singh, V. 2006. Corn wet fractionation prior to fermentation for recovery of valuable co-products. Proceedings of the Corn Utilization and Technology Conference, June 5-7, 2006 in Dallas, Texas.
Protein Distribution in Commercial Wet and Dry-Milled Corn Germ - (Peer Reviewed Journal)
Parris, N., Moreau, R., Johnston, D. B., Singh, V. and Dickey, L.C., 2006. Protein Distribution in Commercial Wet- and Dry-Milled Corn Germ. Journal of Agricultural and Food Chemistry. 54:4868-4872.
Modeling the Process and Costs of the Production of Fuel Ethanol by the Corn Dry-Grind Process - (Peer Reviewed Journal)
Kwiatkowski, J.R., Mcaloon, A.J., Taylor, F., Johnston, D. (2006)Modeling the process and costs of the production of fuel ethanol by the corn dry-grind process. Industrial Crops and Products. V.23, Issue 3, p.288-296.
Protein Distribution in Commercial Wet and Dry-Milled Corn Germ - (Abstract)
Parris, N., Moreau, R.A., Johnston, D., Singh, V., Dickey, L.C. 2006.Protein distribution in commercial wet- and dry-milled corn germ. Presentation at the AOCS Annual meeting, April 30-May 4, 2006, St. Louis, MO., Protein and Co-Products Poster.
Influence of Moisture Content and Cooking on the Bench Scale Screw Pressing of Corn Oil - (Trade Journal)
Moreau, R.A., Johnston, D., Hicks, K.B. Influence of moisture content and cooking on the bench scale screw pressing and pre-pressiing of corn oil from corn germ. Oil Mill Gazetteer 111:8-10 March 2006.
Improvement in Fermentation Characteristics of Degermed Ground Corn by Lipid Supplementation - (Peer Reviewed Journal)
Murthy, G., Singh, V., Johnston, D., Rausch, K.D., Tumbleson, M.E. 2006. Improvement in fermentation characteristics of degermed ground corn by lipid supplementation. 2006. Journal of Industrial Microbiology Biotechnology 33:655-660.
The Role of Lipid and Protein Components in the Emulsification Properties of Gum Arabic and Corn Fiber Gum - (Peer Reviewed Journal)
Yadav, M.P., Johnston, D., Hicks, K.B., Nothnagel, E.A. 2006. The role of lipid and protein components in the emulsification properties of gum arabic and corn fiber gum. Foods & Food Ingredients Journal of Japan. V.211, No. 3.p.243-254.
Structure/function Relationships Between Corn Kernel Arabinoxylans and Their Emulsifying Properties - (Abstract)
Yadav, M.P., Johnston, D., Hicks, K.B. 2005. Structure/function relationships between corn kernel arabinoxylans and their emulsifying properties. Meeting Abstract #225. National Meeting and Exposition Program 229th ACS National Meeting, March 13-17, 2005. San Diego, CA.
A Comparison of Commercial Enzymes for the Aqueous Enzymatic Extraction of Corn Oil from Corn Germ - (Peer Reviewed Journal)
Moreau, R.A., Johnston, D., Powell, M.J., Hicks, K.B. 2004. A comparison of commercial enzymes for the aqueous enzymatic extraction of corn oil from corn germ. Journal of the American Oil Chemists' Society, V.81, No. 11, p.1071-1075.
The Influence of Moisture Content and Cooking on the Screw Pressing and Pre-Pressing of Corn Oil from Corn Germ - (Peer Reviewed Journal)
Moreau, R.A., Johnston, D., Hicks, K.B. 2005. The influence of moisture content and cooking on the screw pressing and pre-pressing of corn oil from corn germ. Journal of the American Oil Chemists' Society. Vol. 82, No. 11,p.851-854.
Comparison of Enzymatic (E-Mill) and Conventional Dry-Grind Corn Processes Using a Granular Starch Hydrolyzing Enzyme - (Peer Reviewed Journal)
Wang, P., Singh, V., Xu, L., Johnston, D., Rausch, K.D., Tumbleson, M.E. 2005. Comparison of enzymatic (e-mill) and conventional dry-grind corn processes using a granular starch hydrolyzing enzyme. 2005. Cereal Chemistry.82(6):734-738.
Composition and Economic Comparison of Germ Fractions Derived from Modified Corn Processing Technologies - (Peer Reviewed Journal)
Johnston, D., Mcaloon, A.J., Moreau, R.A., Hicks, K.B., Singh, V. Composition and economic comparison of germ fractions derived from modified corn processing technologies. 2005. Journal of the American Oil Chemists' Society.82, No. 8, p.603-608.
The Role of Protein and Lipid Components in the Emulsification Properties of Corn Fiber Gum and Gum Arabic - (Abstract)
Yadav, M.P., Johnston, D., Hicks, K.B., Nothnagel, E.A. The role of protein and lipid components in the emulsification properties of corn fiber gum and gum arabic. Meeting Abstract, for the International Hydrocolloids Forum on June 27-28, 2005 at North East Wales Institute of Higher Education, Wrexham, North Wales, U.K. Paper #16, p.21.
Hulless Barley - a New Feedstock for Fuel Ethanol? - (Proceedings/Symposium)
Hicks, K.B., Flores, R.A., Taylor, F., Mcaloon, A.J., Moreau, R.A., Johnston, D., Senske, G.E., Brooks, W.S., Griffey, C.A. 2005. Hulless barley - a new feedstock for fuel ethanol?. Proceedings of the 15th Annual EPAC Ethanol lConference, Cody, WY. June 12-14.
Processes for Recovery of Corn Germ and Optionally Corn Coarse Fiber (Pericarp) - (Patent Application)
Johnston, D., Singh, V. 2005. Processes for recovery of corn germ and optionally corn coarse fiber (pericarp). US Patent #6,899,910 B2. May 31, 2005.
Barley: a Potential Feedstock for Fuel Ethanol in the U. S. - (Proceedings/Symposium)
Hicks, K.B., Flores, R.A., Taylor, F., Mcaloon, A.J., Moreau, R.A., Johnston, D., Senske, G.E., Brooks, W.S., Griffey, C.A. 2005. Barley: a potential feedstock for fuel ethanol in the U. S.?. Proceedings of the Fourth International Starch Conference, Univ. of Illinois, Urbana, IL, June 5-8, p.25-29.
Hydrolysis and Fermentation of Pericarp and Endosperm Fiber Recovered from Enzymatic Corn Dry Grind Process - (Peer Reviewed Journal)
Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A., Singh, V. 2005. Hydrolysis and fermentation of pericarp and endosperm fibers recovered from enzymatic corn dry-grind process. Cereal Chemistry. 82(5):616-620.
Enzymatic Milling Product Yield Comparison with Reduced Levels of Bromelain and Varying Levels of Sulfur Dioxide - (Peer Reviewed Journal)
Johnston, D., Singh, V. Enzymatic milling product yield comparison with reduced levels of bromelain and varying levels of sulfur dioxide. 2005. Cereal Chemistry,V.82(5)p.523-527.
The Influence of Moisture Content and Cooking on the Screw Pressing of Corn Oil from Corn Germ - (Abstract)
Moreau, R.A., Johnston, D., Dickey, L.C., Hicks, K.B. The influence of moisture content and cooking on the screw pressing of corn oil from corn germ. Meeting Abstract. 96th AOCS Annual Meeting & Expo. Salt Lake City, UT, May 1-5, 2005, Processing Poster 1.
Aqueous Enzymatic Extraction of Corn Oil from Corn Germ - (Abstract)
Moreau, R.A., Powell, M.J., Johnston, D., Hicks, K.B. Aqueous enzymatic extraction of corn oil from corn germ. Meeting Abstract. 96th AOCS Anual Meeting & Expo., Salt Lake City, UT, May 1-5, 2005. Biotechnology Poster 1.
Structure/function Relationships Between Corn Fiber Gums and Their Emulsifying Properties - (Abstract)
Yadav, M.P., Johnston, D., Hicks, K.B., Nothnagel, E.A. 2005. Structure/function relationships between corn fiber gums and their emulsifying properties. Meeting Abstract, Univ. of California, Riverside, March 18, 2005.
Review of Process for Producing Corn Fiber Oil and Ethanol from "quick Fiber" - (Review Article)
Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Tucker, M.P., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2005. Review of process for producing corn fiber oil and ethanol from "Quick Fiber." International Sugar Journal. 107(1275):187-191.
Comparison of Modified Dry-Grind Corn Processes for Fermentation Characteristics and Ddgs Composition - (Peer Reviewed Journal)
Singh, V., Johnston, D., Naidu, K., Rausch, K.D., Belyea, R.L., Tumbleson, M.E. 2005. Comparison of modified dry-grind corn processes for fermentation characteristics and ddgs composition. Cereal Chemistry 82(2):187-190.
Particle Size Distribution of Ground Corn and Ddgs from Dry Grind Processing - (Peer Reviewed Journal)
Rausch, K.D., Belyea, R.L., Ellersieck, M.R., Singh, V., Johnston, D., Tumbleson, M.E., 2005. Particle size distribution of ground corn and ddgs from dry grind processing. American Society of Agricultural Engineers. V. 48(1):p.273-277.
A Comparison Between Conversion of Pericarp and Endosperm Fiber from Corn into Ethanol - (Proceedings/Symposium)
Singh, V., Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A. 2004. A comparison between conversion of pericarp and endosperm fiber from corn into ethanol. Proceedings of ASAE Annual International Meeting. p. 1-15.
Composition and Economic Comparison of Germ Fractions Derived from Modified Corn Processing Technologies - (Abstract)
Johnston, D., Mcaloon, A.J., Moreau, R.A., Hicks, K.B., Singh, V. Composition and economic comparison of germ fractions derived from modified corn processing technologies. Meeting Abstract 2004. International Fuel Ethanol Workshop & Expo. Kansas City, MO, June 28-July 1. Track 3: Dry Mill Ethanol lPOroduction Modifications (1).
A Two-Liter Dry Grind Laboratory Procedure to Measure Sugar Profile and Ethanol Yields - (Abstract)
Naidu, K., Rausch, K.D., Johnston, D., Tumbleson, M.E., Singh, V. A two-liter dry grind laboratory procedure to measure sugar profile and ethanol yields. Proceedings of the Corn Utilization & Technology Conference, Indianapolis, IN., June 7-9, 2004. Poster Presentation CD Rom.
Convention Corn Wet Milling Process and Cost Models - (Abstract)
Johnston, D., Yee, W.C., Singh, V., Mcaloon, A.J. Convention corn wet milling process and cost models. Proceedings of the Corn Utilization & Technology Conference. Indianapolis, IN., June 7-9, 2004. Poster Presentation CD Rom.
The Effects of Processing Chemicals on Corn Starch Pasting Properties - (Abstract)
Johnston, D., Thomas, J.L. The effects of processing chemicals on corn starch pasting properties. Proceedings of the Corn Utilization & Technology Conference, Indianapolis, IN., June 7-9, 2004. Poster Presentation CD Rom.
Effect of Modified Dry Grind Corn Processes on Fermentation Characteristics and Ddgs Composition - (Abstract)
Singh, V., Johnston, D., Naidu, K., Rausch, K.D., Belyea, R.L., Tumbleson, M.E. Effect of modified dry grind corn processes on fermentation characteristics and ddgs composition. Proceedings of the Corn Utilization & Technology Conference, Indianapolis, IN. June 7-9, 2004. Poster Presentation CD Rom.
Comparison of Dry and Wet Milling Degerm and Defiber Processes for Ethanol Production - (Abstract)
Murthy, G.S., Johnston, D., Singh, V. Comparison of dry and wet milling degerm and defiber processes for ethanol production. Proceedings of the Corn Utilization & Technology Conference, Indianapolis, IN. June 7-9, 2004, Poster CD.
Effect of Effect of Endosperm Hardness on Sugar Profiles and Ethanol Yields in Dry Grind Ethanol Process - (Abstract)
Murthy, G.S., Rausch, K.D., Johnston, D., Tumbleson, M.E., Singh, V. Effect of effect of endosperm hardness on sugar profiles and ethanol yields in dry grind ethanol process. Proceedings of the Corn Utilization & Technology Conference, Indianapolis, IN. June 7-9, 2004. Poster CD.
Developing Barley As a Major Feedstock for Fuel Ethanol Production - (Abstract)
Hicks, K.B., Taylor, F., Kohout, K.M., Kurantz, M.J., Thomas, J.L., O Brien, D.J., Johnston, D., Flores, R.A., Moreau, R.A., Hoot, L. Developing barley as a major feedstock for fuel ethanol production. Meeting Abstract 2004. International Fuel Ethanol Workshop & Trade Show. Madison, WI., June 22-25. Session 7: Feedstocks for Ethanol Production (1).
Aqueous Enzymatic Extraction of Corn Oil from Corn Germ - (Abstract)
Moreau, R.A., Johnston, D., Hicks, K.B. 2004. Aqueous enzymatic extraction of corn oil from corn germ. Meeting Abstract. Conference proceedings, Corn Utilization and Technology Conference, June 7-9, 2004, Indianapolis, IN., Poster 2
Starch Production from An Enzymatic Maize Wet Milling Process: Enzyme and Process Optimization - (Abstract)
Johnston, D., Singh, V. Starch production from an enzymatic maize wet milling process: enzyme and process optimization. Meeting Abstract.55th Annual Starch Conference, Detmold Germany, April 20-24, 2004. Paper #3.1.
Enzymatic Maize Milling Process for Dry Grind Ethanol - (Abstract)
Singh, V., Johnston, D., Rausch, K.D. Enzymatic maize milling process for dry grind ethanol. Meeting Abstract. 55th Annual Starch Conference, Detmold Germany, April 20-24, 2004. Paper #3.6.
Isolation of Zeins from Enzymatically Milled Corn - (Abstract)
Parris, N., Singh, V., Johnston, D. 2004. Isolation of zeins from enzymatically milled corn. Meeting Abstract. Corn Utilization & Technology Conference, June 7-9, 2004, Indianapolis, IN. p.41.
Enzymatic Milling of Corn: Optimization of Enzymes Addition and First Grind - (Peer Reviewed Journal)
Johnston, D., Singh, V. 2004. Enzymatic milling of corn: optimization of enzymes addition and first grind. Cereal Chemistry. Cereal Chemistry 81(5):p.626-632
Fermentation of "quick Fiber" Produced from a Modified Corn Milling Process into Ethanol and Recovery of Corn Fiber Oil - (Peer Reviewed Journal)
Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2004. Fermentation of "quick fiber" produced from a modified corn milling process into ethanol and recovery of corn fiber oil. Journal of Applied Biochemistry and Biotechnology. 115:937-950.
Use of Enzymes to Reduce Steep Time and So2 Requirements in a Maize Wet-Milling Process - (Patent Application)
Patent issued 05/20/2003. 115's not required at time patent application was done. Patent #6,566,125 B2.
Recovery of Nutraceuticals and Gums from Corn Fiber - (Proceedings/Symposium)
Moreau, R.A., Hicks, K.B., Johnston, D., Singh, V. 2003. Recovery of nutraceuticals and gums from corn fiber. Proceedings in Third International Starch Technology Conference, University of Illinois, Urbana, p.2-9, 2003.
Pre-Treatment of Wet-Milled Corn Fiber to Improve Recovery of Corn Fiber Oil, and Phytosterols - (Peer Reviewed Journal)
Cereal Chem. 80:118-122, 2003
Potential New Uses for Corn Fiber - (Proceedings/Symposium)
Proceedings of the Corn Utilization & Technology Conference, June 3-5, 2002 (2002) pp.122-127. Published by the National Corn Growers Assoc. & Corn Refiners Assoc. Inc.

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Last Modified: 11/24/2009
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