Projects

Biology, Biological Control, and Molecular Genetics of Root Diseases of Wheat, Barley and Biofuels Brassicas Appropriated (D)   Accession Number: 411693

Identification of Lethal Determinants in Pseudomonas Sp. for Plant-Parasitic Nematode Control Reimbursable (R)   Accession Number: 411636

Publications

Rhizoctonia Resistant Wheat -- Potential New Resources for Control for Soilborne Pathogens. - (Abstract) - (20-May-09)

Ten Years of Plant Pathology Research at the Cook Agronomy Farm: What Have We Learned? - (Abstract)

Paulitz, T.C., Schroeder, K.L., Okubara, P.A. 2009. Ten Years of Plant Pathology Research at the Cook Agronomy Farm: What Have We Learned?. Meeting Abstract. Timely Solutions for Shifting Economics. Department of Crops and Soil Sciences Technical Report 09-1

Real-time detection and quantification of Rhizoctonia and Pythium species on the Cook Agronomy Farm. - (Abstract)

Schroeder, K.L., Paulitz, T.C., Okubara, P.A. 2009. Real-time detection and quantification of Rhizoctonia and Pythium species on the Cook Agronomy Farm. Meeting Abstract.

Incidence and spatial distribution of Rhizoctonia and Pythium species determined with real-time PCR - (Abstract)

Schroeder, K.L., Paulitz, T.C., Okubara, P.A. 2009. Incidence and spatial distribution of Rhizoctonia and Pythium species determined with real-time PCR. Phytopathology 99: S115.

Application of real-time PCR for quantification of soilborne pathogens - (Abstract)

Schroeder, K.L., Okubara, P.A., Paulitz, T.C. 2009. Application of real-time PCR for quantification of soilborne pathogens. Phytopathology 99: S186.

EMS-treated hexaploid wheat genotype Scarlet has enhanced tolerance to the soilborne necrotrophic pathogens Rhizoctonia solani AG-8 and R. oryzae. 2009. Theor. Appl. Genet. 119(February): 293-303 - (Peer Reviewed Journal)

Okubara, P.A., Steber, C.M., Demacon, V.L., Walter, N., Paulitz, T.C., Kidwell, K.K. EMS-treated hexaploid wheat genotype Scarlet has enhanced tolerance to the soilborne necrotrophic pathogens Rhizoctonia solani AG-8 and R. oryzae. 2009. Theor. Appl. Genet. 119(February): 293-303. Theoretical and Applied Genetics.

Controlling Soilborne Pathogens in Wheat Production Systems - (Abstract)

Okubara, P.A., Schroeder, K.L., Paulitz, T.C., Steber, C.M., Kidwell, K. 2008. Controlling Soilborne Pathogens in Wheat Production Systems. Washington State University College of Agriculture and Home Economics. Page 37.

Integrated control of soilborne plant pathogens - (Abstract)

Paulitz, T.C., Schroeder, K.L., Okubara, P.A. 2008. Integrated control of soilborne plant pathogens. Journal of Plant Pathology. Vol. 90 (2, Supplement) Aug. 2008 S2.62

Biology, Biological Control and Molecular Genetics of Root Diseases of Wheat and BARLEY. BIOLOGY, Biological Control and Molecular Genetics of Root Diseases of Wheat and BARLEY. - (Abstract)

Mavrodi, D., Mavrodi, O., Bonsall, R., Paulitz, T.C., Okubara, P.A., Schroeder, K.L., Kwak, Y., Weller, D.M. 2008. Biology, biological control and molecular genetics. Vol. 90 (2 Supplement) Aug. 2008 S 2.18. of root diseases of wheat and barley. biology, biological control and molecular genetics of root diseases of wheat and barley. . Journal of Plant Pathology.

Improved quantification of pathogen DNA from soil using pressure cycling technology - (Abstract)

Okubara, P.A., Schroeder, K.L., Li, C., Schumacher, R.T., Lawrence, N.P. 2008. Improved quantification of pathogen DNA from soil using pressure cycling technology. International Congress of Plant Pathology Abstracts and Proceedings.

EMS-generated Rhizoctonia resistance in an adapted wheat - (Abstract)

Okubara, P.A., Steber, C.M., Paulitz, T.C., Kidwell, K.K. 2008. Ems-generated rhizoctonia resistance in an adapted wheat. Journal of Plant Pathology. S.350.(2)

Biology, biological control and molecular genetics of root diseases of wheat and barley - (Abstract)

Thomashow, L.S., Mavrodi, D., Mavrodi, O., Bonsall, R., Paulitz, T.C., Okubara, P.A., Schroeder, K.L., Quak, Y., Weller, D.M. 2008. Biology, biological control and molecular genetics of root diseases of wheat and barley. Plant Pathology. S(2).18.

Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil - (Peer Reviewed Journal)

Yan, G., Smiley, R., Okubara, P.A., Skantar, A.M., Easley, S.A., Sheedy, J.G., Thompson, A.L. 2008. Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil. Plant Disease. 92(11):1480-1487.

Accumulation of Pseudomonas-Derived 2,4-Diacetylphloroglucinol on Wheat Seedling Roots Is Influenced by Host Cultivar-Bacterial Strain Interaction. - (Peer Reviewed Journal)

Okubara, P.A., Bonsall, R.F. 2008. Accumulation of pseudomonas-derived 2,4-diacetylphloroglucinol on wheat seedling roots is influenced by host cultivar-bacterial strain interaction. Biological Control. 46(2008)322-331.

Identification and Quantification of Pathogenic Rhizoctonia solani and R. oryzae Using Real-time PCR - (Peer Reviewed Journal)

Okubara, P.A., Schroeder, K., Paulitz, T.C. 2008. Identification and Quantification of Pathogenic Rhizoctonia solani and R. oryzae Using Real-time PCR. Phytopathology. Vol. 98, No. 7, 2008. p. 837-847.

Characterization of the Pseudomonas genus of bacteria for plant-parasitic nematode control - (Abstract)

Aly, H., Kamalay, J., Walter, N., Okubara, P.A., Taylor, C.G. 2007. Characterization of the Pseudomonas genus of bacteria for plant-parasitic nematode control. ASM Conference.

Pressure Cycling Technology Sample Preparation System (PCT SPS) Improves Quantification of Pathogen DNA in Plants and Soil - (Abstract) - (28-Jul-07)

Geographic distribution of Rhizoctonia and Pythium species in soils throughout eastern Washington. - (Abstract)

Schroeder, K.L., Paulitz, T.C., Okubara, P.A. 2007. Geographic distribution of Rhizoctonia and Pythium species in soils throughout eastern Washington.. Joint Meeting of the ADSA, AMSA, ASAS and PSA.

Virus Induced Gene Silencing for Functional Characterization of Genes Expressing in Various Wheat Tissues - (Abstract)

Bennypaul, H.S., Gill, U.S., Okubara, P.A., Gill, K.S. 2007. Virus Induced Gene Silencing for Functional Characterization of Genes Expressing in Various Wheat Tissues. Plant and Animal Genome VX Conference Abstracts.

The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight - (Abstract)

Alexander, N.J., Blechl, A.E., Okubara, P.A., Mc Cormick, S.P., Manoharan, M., Dahleen, L.S., Kendra, D.F. 2006. The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight [abstract]. World Mycotoxin Forum, the Fourth Conference. p. 123.

Pressure Cycling Technology Sample Preparation System (PCT SPS) Improves Quantification of Pathogen DNA in Plants and Soil - (Abstract)

Okubara, P.A., Li, C., Lawrence, N. 2007. Pressure Cycling Technology Sample Preparation System (PCT SPS) Improves Quantification of Pathogen DNA in Plants and Soil. Plant and Animal Genome VX Conference Abstracts.

Molecular Responses of Wheat to Soilborne Fungal Pathogens. - (Abstract)

Okubara, P.A. 2006. . .

Characterization of Pathogenic Rhizoctonia Solani and R. Oryzae of the Pacific Northwest Using Real-Time Pcr - (Abstract)

Okubara, P.A., Paulitz, T.C. Characterization of pathogenic rhizoctonia solani and r. oryzae of the pacific northwest using real-time pcr. American Phytopathological Society Annual Meeting. June 15, Abstract #31, p.9.

Dissection of Wheat Genes Expressed During Rhizosphere Interactions with Biocontrol Pseudomonas Fluorescens - (Abstract)

Okubara, P.A., Kwak, Y., Walter, N., Call, D., Skinner, D.Z. Dissection of wheat genes expressed during rhizosphere interactions with biocontrol pseudomonas fluorescens. Annual Missouri Symposium. Abstract #17, p. 43-44

First Report of Damping-off of Canola Caused by Rhizoctonia Solani Ag 2-1 in Washington State - (Peer Reviewed Journal)

Paulitz, T.C., Okubara, P.A., Schillinger, W.F. 2006. First report of damping-off of canola caused by rhizoctonia solani ag 2-1 in washington state. Plant Disease. Vol. 90: 829.

Identification and Quantification of Pathogenic Pythium Spp. from Soils in Eastern Washington Using Real-Time Pcr. - (Peer Reviewed Journal)

Schroeder, K.L., Okubara, P.A., Tambong, J.T., Paulitz, T.C. 2006. Identification and quantification of pathogenic pythium spp. from soils in eastern washington using real-time pcr.. Phytopathology. Vol. 96: 637-647.

Y15_999fw, a Dominant Scar Marker Linked to the Fusarium Wilt Race 1 (Fw) Resistance Gene in Pea - (Peer Reviewed Journal)

Okubara, P.A., Keller, K.E., Mcclendon, M.T., Inglis, D.A., Mcphee, K.E., Coyne, C.J. 2005. Y15_999fw, a dominant scar marker linked to the fusarium wilt race 1 (fw) resistance gene in pea. Pisum Genetics. Vol.37, pp32-35.

Molecular Bases of Root Defense Responses. - (Peer Reviewed Journal)

Okubara, P.A., Paulitz, T.C. 2005. Molecular bases of root defense responses.. Plant and Soil Journal. 274:221,232.

Interaction Between Wheat Cultivar and Bacterial Isolate for Rhizosphere Colonization and Root Accumulation of Pseudomonas-Derived 2,4-Diacetylphloroglucinol - (Abstract)

Okubara, P.A., Mitchell, J., Berghammer, M.J., Bonsall, R. 2005. Interaction between wheat cultivar and bacterial isolate for rhizosphere colonization and root accumulation of pseudomonas-derived 2,4-diacetylphloroglucinol. Can. J. Plant Pathology. 27: 474

Interaction Between Wheat and Bacterial Genotypes for Rhizosphere Colonization and Root Accumulation of Pseudomonas-Derived 2,4-Diacetylphloroglucinol - (Abstract) - (20-Jun-05)

Interaction Between Wheat Cultivar and Bacterial Isolate for Rhizosphere Colonization and Root Accumulation of Pseudomonas-Derived 2,4-Diacetylphloroglucinol - (Abstract) - (15-Jun-05)

Real-Time Pcr: Applications to Studies on Soilborne Pathogens - (Peer Reviewed Journal)

Okubara, P.A., Schroeder, K.L., Paulitz, T.C. 2005. Real-time pcr: applications to studies on soilborne pathogens. Canadian Journal of Plant Pathology. Can. J. Plant Pathology. 27: 300-313.

New Approaches to Overcome Fusarium Head Blight - (Proceedings/Symposium)

Blechl, A.E., Somleva, M.N., Okubara, P.A., Dill-Mackey, R. 2005. New approaches to overcome fusarium head blight. International Wheat Quality Conference. p. 85-95.

Long Oligonucleotide Microarrays in Wheat: Evaluation of Hybridization signal Amplification and An Oligonucleotide-Design Computer Script - (Peer Reviewed Journal)

Skinner, D.Z., Okubara, P.A., Hyun-Baek, K., Call, D.R. 2005. Long oligonucleotide microarrays in wheat: evaluation of hybridization signal amplification and an oligonucleotide-design computer script. Functional and Integrative Genomics. Vol 5, Number 2, pgs 70-79.

Simultaneous Identification and Quantification of Rhizoctonia Solani and R. Oryzae from Root Samples Using Real-Time Pcr. - (Abstract)

Okubara, P.A., Paulitz, T.C. 2004. Simultaneous identification and quantification of rhizoctonia solani and r. oryzae from root samples using real-time pcr. American Phytopathological Society Annual Meeting. Phytopathology 94: S77.

Microarray Expression Profiles of Wheat Roots and Shoots During Cold Stress and Root Colonization by Biocontrol Pseudomonas Fluorescens - (Abstract)

Okubara, P.A., Skinner, D.Z. 2004. Microarray expression profiles of wheat roots and shoots during cold stress and root colonization by biocontrol pseudomonas fluorescens. American Society of Plant Biologists Annual Meeting. Plant Biology 2004, Abstract No. 694.

Rhizoshpere Colonization of Hexaploid Wheat by Pseudomonas Fluorescens Strains Q8r1-96 and Q2-87 Is Cultivar-Variable and Associated with Changes in Gross Root Morphology. - (Peer Reviewed Journal)

Okubara, P.A., Kornoely, J.P., Landa, B.B. 2003. Rhizoshpere colonization of hexaploid wheat by pseudomonas fluorescens strains q8r1-96 and q2-87 is cultivar-variable and associated with changes in gross root morphology.. Biological Control 30 (2004) 392-403.

Root Responses to Necrotrophic Pathogens and Parasites - Defense Molecules, Genes, and Pathways - (Book/Chapter) - (17-Sep-03)

Root Defense Responses to Pathogens and Parasites: a Molecular perspective - (Peer Reviewed Journal)

OKUBARA, P.A., PAULITZ, T.C. ROOT DEFENSE RESPONSES TO PATHOGENS AND PARASITES: A MOLECULAR PERSPECTIVE. PLANT AND SOIL JOURNAL. 2003.

Functional Diversity of Est's from Stressed and Non-Stressed Wheat Roots - (Abstract)

OKUBARA, P.A. FUNCTIONAL DIVERSITY OF EST'S FROM STRESSED AND NON-STRESSED WHEAT ROOTS. AMERICAN SOCIETY OF PHOTOBIOLOGISTS PROCEEDINGS. p. 201. 2003Abstract No. 986.

Partial Retrotransposon-Like DNA Sequence in the Genomic Clone of Aspergillus Flavus, Paf28 - (Peer Reviewed Journal)

Okubara, P.A., Tibbot, B.K., Tarun, A.S., Mcalpin, C.E., Hua, S.T. Partial retrotransposon-like dna sequence in the genomic clone of aspergillus flavus, PAF28. Mycological Research. v.107. p. 841-846. 2003.

Transgene Expression in Spring Wheat (Triticum Aestivum L.) Transformed with Candidate Anti-Fusarium Genes - (Proceedings/Symposium)

Somleva, M., Okubara, P.A., Blechl, A.E. 2002. Transgene expression in spring wheat (triticum aestivum l.) transformed with candidate anti-fusarium genes. National Fusarium Head Blight Forum Proceedings. 42-45

A Novel Rapd Marker Linked to the Fusarium Wilt Race 5 Resistance Gene (Fwf) in Pisum Sativum - (Peer Reviewed Journal)

Okubara, P.A., Inglis, D.A., Muehlbauer, F.J., Coyne, C.J. A novel RAPD marker linked to the Fusarium wilt race 5 resistance gene (Fwf) in pisum sativum. Pisum Genetics 34:6-8.

Wheat Rhizosphere-Microbe Interactions-Factors and Genes - (Other)

OKUBARA, P.A., LANDA, B.B., KORNOLEY, J.K., PAULITZ, T.C. WHEAT RHIZOSPHERE-MICROBE INTERACTIONS--FACTORS AND GENES. INTERNATIONAL CONGRESS OF PLANT PATHOLOGY ABSTRACTS AND PROCEEDINGS. v. 2. p. 205. 2003.

Engineering Deoxynivalenol Metabolism in Wheat Through the Expression of a Fugal Trichothecene Acetyltransferase Gene - (Peer Reviewed Journal) - (05-Apr-02)