Projects

Evolutionary Enzymes and Separation Processes for Improved Biorefining of Crops and Residues Appropriated (D)   Accession Number: 409351

Publications

Molecular cloning and characterization of multidomain xylanase from manure library - (Peer Reviewed Journal)

Li, R., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2009. Molecular cloning and characterization of multidomain xylanase from manure library. World Journal of Microbiology and Biotechnology. doi:10:1007/s11274-009-011106

Engineer Sccharomyces cerevisiae for consolidated bioprocessing - (Abstract)

Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.

Cloning biomass-degrading enzymes from environmental sources - (Abstract)

Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.

Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08 - (Peer Reviewed Journal)

Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10

Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture - (Peer Reviewed Journal)

Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.

Metagenomic gene discovery and enzyme engineering for biomass conversion - (Abstract)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.

Gene discovery and engineering for biomass degradation - (Proceedings/Symposium)

Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51.

Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme - (Peer Reviewed Journal)

Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305.

Biofuels Group NSF DUSEL Project - (Abstract)

Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.

Cloning and characterization of xylanase gene from hot spring microorganism - (Abstract)

Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.

Developing enzyme systems for biomass deconstruction - (Abstract)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217

Satb1 Packages Densely Looped, Transcriptionally Active Chromatin for Coordinated Expression of Cytokine Genes - (Peer Reviewed Journal)

Cai, S., Lee, C.C., Kohwi-Shigematsu, T. 2006. Satb1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. Nature Genetics. (38):1278-1288

Expression and Secretion of Clostridium Cellulovorans Xyna in Saccharomyces Cerevisiae - (Abstract)

Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220.

Genetic and Biochemical Characterization of a Novel Bacterial A-L-Arabinofuranosidase - (Peer Reviewed Journal)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.

Evolutionary Enzyme Design for Improved Biorefining of Crops and Residues - (Abstract)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.

Synergistic Action of Barley Alpha-Amylase and Lentinula Edodes Glucoamylase on Raw Starch Hydrolysis - (Abstract)

Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.

Cloning and Characterization of Cold-Active Xylanase Enzymes - (Abstract)

Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.

Cloning and Characterization of a Cold Active Xylanase Enzyme - (Peer Reviewed Journal)

Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.

Native Or Raw Starch Digestion - (Review Article)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.

Isolation and Characterization of a Psychrophilic Xylanase Enzyme from Flavobacterium Sp. - (Peer Reviewed Journal)

Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.

Characterization of Active Lentinula Edodes Glucoamylase Expressed and Secreted by Saccharomyces Cerevisiae - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.

Characterization and Directed Evolution of Hemicellulotic Enzymes Using Natural Xylooligoaccharide Substrates - (Abstract)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.

Enzyme-Coupled Assay for Beta-Xylosidase Hydrolysis of Natural Substrates - (Peer Reviewed Journal)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.

Evolved Enzymes for Cold Or Non-Cooking Hydrolysis of Starches to Fermentation Feedstock - (Abstract)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.

Expression and Characterization of Active Lentinus Edodes Glucoamylase in Saccharomyces Cerevisiae - (Abstract)

Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.

Cloning and Characterization of Biomass Degrading Enzymes - (Abstract)

Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).

Directed Evolution of Barley Alpha-Amylase for Increased Activity - (Abstract)

Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).

Heterogeneous Biocatalyic Liquefaction and Saccharificaation of Starch for Ethanol - (Abstract)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.

In Vitro Evolution of High-Activity Barley Alpha-Amylase for Raw Starch Digestion - (Abstract)

Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California. Abstract.

High-Activity Barley Alpha-Amylase by Directed Evolution - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.

Rapid Selection of Alpha-Amylase Mutants in Yeast Libraries for Raw Starch Activity - (Abstract)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity. 2003. Society of Biomolecular Screening Annual Conference, Portland, Oregon.

Engineering Apha-Amylase for Enhanced Activity by Molecular Evolution - (Abstract)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution. American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts. Abstract No. B38.

An Episomal Expression Vector for Screening Mutant Gene Libraries in Pichia Pastoris - (Peer Reviewed Journal)

Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004. An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.

Direct Screening of Libraries of Yeast Clones for Alpha-Amylase Activity on Raw Starch - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.

Cloning and Characterization of the Xyn11a Gene from Lentinula Edodes - (Peer Reviewed Journal)

Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.