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United States Department of Agriculture

Agricultural Research Service

RAYMOND P GLAHN
Plant, Soil and Nutrition Research
Research Physiologist (Animals)

Phone: (607) 255-2452
Fax: (607) 255-1132
Room 203
USDA-ARS, ROBERT W. HOLLEY CENTER, TOWER ROAD
ITHACA, NY, 14853-2901

Projects
Bioavailability of Iron, Zinc and Select Phytochemicals for Improved Health
Appropriated (D)
  Accession Number: 426380
IMPROVING THE IRON BIOAVAILABILITY FROM STAPLE FOOD CROPS, FOOD PRODUCTS, AND FOOD INGREDIENTS
Specific Cooperative Agreement (S)
  Accession Number: 419958
Iron Biofortification of Lentils: Defining Current Production of High FE Lentils and Development of Enhanced Nutritional Quality, BRE1119
Trust (T)
  Accession Number: 419531

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Hydrolysis of soybean protein improves iron bioavailability - (Peer Reviewed Journal)
Lv, Y., Shuntang, G., Zhiqiang, C., Glahn, R.P., Tako, E.N. 2014. Hydrolysis of soybean protein improves iron bioavailability. Food and Nutrition Research. 2(4):162-166. DOI: 10.12691/jfnr-2-4-5.
Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (phaseolus vulgaris L.) Reprint Icon - (Peer Reviewed Journal)
Tako, E.N., Beebe, S.E., Hart, J.J., Reed, S.M., Glahn, R.P. 2014. Polyphenolic compounds appear to limit the nutritional benefit of biofortified higher iron black bean (phaseolus vulgaris L.). Nutrition Journal. 13:28.
Dietary zinc deficiency affects blood linoleic acid: dihomo-gamma-linolenic acid (LA:DGLA) ratio; a reactive physiological marker of zinc status in vivo (Gallus gallus) - (Peer Reviewed Journal)
Reed, S., Xia, Q., Rinat, R., Thomas, B., Glahn, R.P., Tako, E. 2014. Dietary zinc deficiency affects blood linoleic acid: dihomo-gamma-linolenic acid (LA:DGLA) ratio; a reactive physiological marker of zinc status in vivo (Gallus gallus). Nutrients. 6:1164-1180. DOI: 10.3390/nu6031164.
Seed coat removal improves Fe bioavailability in cooked lentils: studies using an in vitro digestion/Caco-2 cell culture model - (Peer Reviewed Journal)
DellaValle, D.M., Vandenberg, A., Glahn, R.P. 2013. Seed coat removal improves Fe bioavailability in cooked lentils: studies using an in vitro digestion/Caco-2 cell culture model. Journal of Agricultural and Food Chemistry. 61(34):8084-8089. DOI: 10.1021/jf4022916.
Bioavailability of iron in geophagic earths and clay minerals, and their effect on dietary iron absorption using an in vitro digestion/Caco-2 cell model Reprint Icon - (Peer Reviewed Journal)
Seim, G., Ahn, C.I., Bodis, M., Luwedde, F., Miller, D., Hillier, S., Tako, E.N., Glahn, R.P., Young, S.L. 2013. Bioavailability of iron in geophagic earths and clay minerals, and their effect on dietary iron absorption using an in vitro digestion/Caco-2 cell model. Food and Function. 4(8):1263-1270.
Soil consumed by chacma baboons is low in bioavailable iron and high in clay - (Peer Reviewed Journal)
Pebsworth, P.A., Seim, G., Huffman, M.A., Glahn, R.P., Tako, E.N., Young, S.L. 2013. Soil consumed by chacma baboons is low in bioavailable iron and high in clay. Journal of Chemical Ecology. http://www.springerlink.com/openurl.asp?genre=article&id=DOI: 10.1007/s10886-013-0258-3.
Lentil (Lens culinaris L) as a candidate crop for iron biofortification: Is there a genetic potential for iron bioavailability? - (Peer Reviewed Journal)
DellaValle, D.M., Thavarajah, D., Thavarajah, P., Vandenberg, A., Glahn, R.P. 2013. Lentil (Lens culinaris L) as a candidate crop for iron biofortification: Is there a genetic potential for iron bioavailability? Field Crops Research. 144:119-125.
High bioavailable iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro and in vivo Reprint Icon - (Peer Reviewed Journal)
Tako, E.N., Hoekenga, O., Kochian, L.V., Glahn, R.P. 2013. High bioavailable iron maize (Zea mays L.) developed through molecular breeding provides more absorbable iron in vitro and in vivo. Nutrition Journal. 12:3.
Oral exposure to polystyrene nanoparticles effects iron absorption Reprint Icon - (Peer Reviewed Journal)
Mahler, G., Esch, M., Tako, E.N., Southard, T., Glahn, R.P., Shuler, M. 2012. Oral exposure to polystyrene nanoparticles effects iron absorption. Nature Nanotechnology. DOI: 10.1038/nnano.2012.3.
Genetic reduction of antinutrients in common bean (Phaseolus vulgaris L.) seed, increases nutrients and in vitro iron bioavailability without depressing main agronomical traits - (Peer Reviewed Journal)
Campion, B., Glahn, R.P., Tava, A., Perrone, D., Doria, E., Sparvoli, F. 2013. Genetic reduction of antinutrients in common bean (Phaseolus vulgaris L.) seed, increases nutrients and in vitro iron bioavailability without depressing main agronomical traits. Field Crops Research. 141:27-37.
Evaluation of metallothionein formation as a proxy for zinc absorption in an in vitro digestion/caco-2 cell culture model - (Peer Reviewed Journal)
Zhiqiang, C., Tako, E.N., Yeung, A., Welch, R., Glahn, R.P. 2012. Evaluation of metallothionein formation as a proxy for zinc absorption in an in vitro digestion/caco-2 cell culture model. Food and Function. 3(7):732-736.
Intra amniotic administration and dietary inulin affect the iron status and intestinal functionality of iron deficient broiler chickens - (Peer Reviewed Journal)
Tako, E.N., Glahn, R.P. 2012. Intra amniotic administration and dietary inulin affect the iron status and intestinal functionality of iron deficient broiler chickens. Poultry Science. 91(6):1361-1370.
Metacridamides A and B, bioactive macrocycles from conidia of the entomopathogenic fungus Metarhizium acridum - (Peer Reviewed Journal)
Krasnoff, S., Englich, U., Miller, P.G., Shuler, M.L., Glahn, R.P., Donzelli, B.G., Gibson, D.M. 2012. Metacridamides A and B, bioactive macrocycles from conidia of the entomopathogenic fungus Metarhizium acridum. Journal of Natural Products. 75:175-180.
Biofortified red mottled beans (phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model - (Peer Reviewed Journal)
Tako, E.N., Blair, M., Glahn, R.P. 2011. Biofortified red mottled beans (phaseolus vulgaris L.) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model. Nutrition Journal. 10:113.
Metacridamides A and B from the biocontrol fungus metarhizium acridum - (Abstract Only)
Krasnoff, S., Englich, U., Miller, P., Shuler, M.L., Glahn, R.P., Donzelli, B.G., Gibson, D.M. 2011. Metacridamides A and B from the biocontrol fungus metarhizium acridum. American Society of Pharmacognosy. p. 325.
Iron status of the late term broiler (Gallus gallus) embryo and hatchling - (Peer Reviewed Journal)
Tako, E.N., Glahn, R.P. 2011. Iron status of the late term broiler (Gallus gallus) embryo and hatchling. International Journal of Poultry Science. 10(1):42-48.
The usefulness of iron bioavailability as a target for breeding maize (Zea mays L.) with enhanced nutritional value - (Peer Reviewed Journal)
Pixley, K., Glahn, R.P., Palacious-Rojas, N. 2011. The usefulness of iron bioavailability as a target for breeding maize (Zea mays L.) with enhanced nutritional value. Field Crops Research. 123(2)153:160.
Genetic and physiological analysis of iron content and bioavailability in maize kernels - (Peer Reviewed Journal)
Lung'Aho, M., Mwaniki, A., Szalma, S.J., Hart, J., Rutzke, M., Kochian, L.V., Glahn, R.P., Hoekenga, O. 2011. Genetic and physiological analysis of iron content and bioavailability in maize kernels. PLoS One. 6(6):e20429. DOI:10.1371/journal.pone.0020429.
The potential of lentil (Lens culinaris L.) as a whole food for increased selenium, iron, and zinc intake: Preliminary results from a three year study Reprint Icon - (Peer Reviewed Journal)
Thavarajah, D., Thavarajah, P., Wejesuriya, A., Rutzke, M., Glahn, R.P., Combs, G.F., Vandenberg, A. 2011. The potential of lentil (Lens culinaris L.) as a whole food for increased selenium, iron, and zinc intake: Preliminary results from a three year study. Euphytica. 180(1):123-128.
Iron biofortification of maize grain - (Peer Reviewed Journal)
Hoekenga, O., Lung'Aho, M., Tako, E., Kochian, L.V., Glahn, R.P. 2011. Iron biofortification of maize grain. Plant Genetic Resources: Characterization and Utilization. 1-3. Available: http://journals.cambridge.org/
White beans provide more bioavailable iron than red beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model - (Peer Reviewed Journal)
Tako, E.N., Glahn, R.P. 2010. White beans provide more bioavailable iron than red beans: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model. International Journal for Vitamin and Nutrition Research. 80(6)416:429.
Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability - (Peer Reviewed Journal)
Tako, E., Rutzke, M.A., Glahn, R.P. 2010. Using the domestic chicken (Gallus gallus) as an in vivo model for iron bioavailability. Poultry Science. 89:514:521.
Use of White Bean Instead of Red Bean May Improve Iron Bioavailability from a Tanzanian Complementary Food Mixture - (Peer Reviewed Journal)
Lung'Aho, M.G., Glahn, R.P. 2010. Use of White Bean Instead of Red Bean May Improve Iron Bioavailability from a Tanzanian Complementary Food Mixture. International Journal for Vitamin and Nutrition Research. 80(1):24-31.
Different responses of Fe transporters in Caco2/HT29-MTX cocultures than in independent Caco-2 cell cultures - (Peer Reviewed Journal)
Laparra, J., Glahn, R.P., Miller, D. 2009. Different responses of Fe transporters in Caco2/HT29-MTX cocultures than in independent Caco-2 cell cultures. Cell Biology International. 33(9):971-977.
Dietary inulin supplementation does not promote colonic iron absorption in a porcine model - (Peer Reviewed Journal)
Patterson, J., Rutzke, M.A., Fubini, S.L., Glahn, R.P., Welch, R.M., Lei, X., Miller, D.D. 2009. Dietary inulin supplementation does not promote colonic iron absorption in a porcine model. Journal of Agricultural and Food Chemistry. 57(12):5250-5256.
Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity - (Peer Reviewed Journal)
Mahler, G., Esch, M.B., Glahn, R.P., Shuler, M.L. 2009. Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity. Biotechnology and Bioengineering. 104(1):193-205.
Comparing soluble ferric pyrophosphate to common iron salts and chelates as sources of bioavailable iron in a caco-2 cell culture model - (Peer Reviewed Journal)
Zhu, L., Glahn, R.P., Nelson, D., Miller, D.D. 2009. Comparing soluble ferric pyrophosphate to common iron salts and chelates as sources of bioavailable iron in a caco-2 cell culture model. Journal of Agricultural and Food Chemistry. 57(11):5014-5019.
Approaching the cellular processes involved in the positive effect of glycosaminoglycans on Fe uptake to Caco-2 cells - (Peer Reviewed Journal) - (05-Apr-09)
Purified glycosaminoglycans from cooked haddock may enhance Fe uptake via endocytosis in a Caco-2 cell culture model - (Peer Reviewed Journal)
Laparra, J., Barbera, R., Alegria, A., Glahn, R.P., Miller, D.D. 2009. Purified glycosaminoglycans from cooked haddock may enhance Fe uptake via endocytosis in a Caco-2 cell culture model. Journal of Food Science. 74(6):H168-173.
Iron and zinc bioavailabilities to pigs from red and white beans (Phaseolus vulgaris L.) are similar - (Peer Reviewed Journal)
Tako, E., Glahn, R.P., Laparra, J.M., Welch, R.M., Lei, X., Kelly, J.D., Rutzke, M.A., Miller, D.D. 2009. Iron and zinc bioavailabilities to pigs from red and white beans (Phaseolus vulgaris L.) are similar. Journal of Agricultural and Food Chemistry. 57(8):3134-3140.
Iron biofortification of maize grain - (Abstract Only)
Hoekenga, O., Lung'Aho, M., Kochian, L.V., Glahn, R.P. 2009. Iron biofortification of maize grain. [abstract]. 51st Annual Maize Genetics Conference.
The use of caco-2 cells in defining nutrient bioavailability: application to iron bioavailability of foods - (Book / Chapter)
Glahn, R.P. 2009. The use of caco-2 cells in defining nutrient bioavailability: application to iron bioavailability of foods. In: McClements, D., Decker, E. Designing functional foods: measuring and controlling food structure breakdown and nutrient absorption. Cambridge, UK: Woodhead Publishing Limited. p. 340-361.
In vitro estimates of iron bioavailability in some Kenyan complementary foods - (Peer Reviewed Journal)
Lung'Aho, M.G., Glahn, R.P. 2009. In vitro estimates of iron bioavailability in some Kenyan complementary foods. Food and Nutrition Bulletin. 30(2):145-152.
Effects of Tea Phenolics on Iron Uptake from Different Fortificants by Caco-2 cells - (Peer Reviewed Journal)
Laparra, J., Glahn, R.P., Miller, D.D. 2009. Effects of Tea Phenolics on Iron Uptake from Different Fortificants by Caco-2 cells. Food Chemistry. 115(3):974-981.
Milk peptides increase iron solubility in water but do not affect DMT-1 expression in Caco-2 cells - (Peer Reviewed Journal)
Argyri, K., Tako, E., Miller, D., Glahn, R.P., Komaitis, M., Kapsokefalou, M. 2009. Milk peptides increase iron solubility in water but do not affect DMT-1 expression in Caco-2 cells. Journal of Agricultural and Food Chemistry. 57(4):1538-1543.
Biofortified black beans (Phaseolus vulgaris L.) in a maize/bean diet provide more bioavailable iron to piglets than standard black beans - (Peer Reviewed Journal)
Tako, E., Laparra, J., Glahn, R.P., Welch, R.M., Lei, X., Beebe, S., Miller, D.D. 2009. Biofortified black beans (Phaseolus vulgaris L.) in a maize/bean diet provide more bioavailable iron to piglets than standard black beans. Journal of Nutrition. 139(2):417-422.
Serum hepcidin is significantly associated with iron absorption from food and supplemental sources in healthy young woman - (Peer Reviewed Journal)
Young, M.F., Glahn, R.P., Ariza-Nieto, M. 2009. Serum hepcidin is significantly associated with iron absorption from food and supplemental sources in healthy young woman. American Journal of Clinical Nutrition. 89(2):533-538.
Assesing potential effects of inulin and probiotic bacteria on Fe bioavailability from common beans (Phaseolus vulgaris L.) to Caco-2 cells - (Peer Reviewed Journal)
Laparra, J., Glahn, R.P., Miller, D. 2009. Assesing potential effects of inulin and probiotic bacteria on Fe bioavailability from common beans (Phaseolus vulgaris L.) to Caco-2 cells. Journal of Food Science. 74(2):H40-46.
Bioaccessibility of phenols in common beans (Phaseolus vulgaris L.) and iron (Fe) availability to Caco-2 cells - (Peer Reviewed Journal)
Laparra, J., Glahn, R.P., Miller, D.D. 2008. Bioaccessibility of phenols in common beans (Phaseolus vulgaris L.) and iron (Fe) availability to Caco-2 cells. Journal of Agricultural and Food Chemistry. 56(22):10999-11005.
Chicken Thigh, Chicken Liver and Iron-Fortified Wheat Flour Optimize Iron Uptake in An Invitro Digestion/caco-2 Cell Model - (Peer Reviewed Journal)
Pachon, H., Stoltzfus, R., Glahn, R.P. 2008. CHICKEN THIGH, CHICKEN LIVER AND IRON-FORTIFIED WHEAT FLOUR OPTIMIZE IRON UPTAKE IN AN INVITRO DIGESTION/CACO-2 CELL MODEL. Nutrition Research. 28(12):851-858.
Isolated Glycosaminoglycans from Cooked Haddock Enhance Nonheme Iron Uptake by Caco-2 cells - (Peer Reviewed Journal)
Laparra, J.M., Tako, E., Glahn, R.P., Miller, D. 2008. Isolated Glycosaminoglycans from Cooked Haddock Enhance Nonheme Iron Uptake by Caco-2 cells. Journal of Agricultural and Food Chemistry. 56(21):10346-10351.
Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model - (Peer Reviewed Journal)
Jin, F., Frohman, C., Glahn, R.P., Thannhauser, T.W., Welch, R.M. 2009. Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model. British Journal of Nutrition. 101(7):312-316.
The bioavailability of iron fortified in whole grain parboiled rice - (Peer Reviewed Journal)
Prom-U-Thai, C., Glahn, R.P., Cheng, Z., Fukai, S., Rerkasem, B., Huang, L. 2009. The bioavailability of iron fortified in whole grain parboiled rice. Food Chemistry. 112(4):982-986.
Homogenization, Lyophilization Or Acid-Extraction of Meat Products Improves Iron Uptake from Cereal-Meat Product Combinations in An in Vitro Digestion/caco-2 Cell Model - (Peer Reviewed Journal)
Pachon, H., Stoltzfus, R., Glahn, R.P. 2009. HOMOGENIZATION, LYOPHILIZATION OR ACID-EXTRACTION OF MEAT PRODUCTS IMPROVES IRON UPTAKE FROM CEREAL-MEAT PRODUCT COMBINATIONS IN AN IN VITRO DIGESTION/CACO-2 CELL MODEL. British Journal of Nutrition. 101(6):816-821.
Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Ferritin-Iron Bioavailability as Determined Using an In Vitro Digestion/Caco-2 Cell Model - (Peer Reviewed Journal)
Jin, F., Welch, R.M., Glahn, R.P. 2009. Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Ferritin-Iron Bioavailability as Determined Using an In Vitro Digestion/Caco-2 Cell Model. British Journal of Nutrition. 101(7):972-981.
Extrinsic Labeling Method May Not Accurately Measure Fe Absorption from Cooked Pinto Beans (Phaseolus vulgaris):Comparison of Extrinsic and Intrinsic labeling of Beans - (Peer Reviewed Journal)
Jin, F., Glahn, R.P., Rutzke, M., Zhiqiang, C., Welch, R.M. 2008. Extrinsic Labeling Method May Not Accurately Measure Fe Absorption from Cooked Pinto Beans (Phaseolus vulgaris):Comparison of Extrinsic and Intrinsic labeling of Beans. Journal of Agricultural and Food Chemistry. 56(16):6881-6885.
Characterization of Caco-2 and HT29-MTX co-cultures in an in vitro digestion/cell culture model used to predict iron bioavailability - (Peer Reviewed Journal)
Mahler, G.J., Shuler, M.L., Glahn, R.P. 2008. Characterization of Caco-2 and HT29-MTX co-cultures in an in vitro digestion/cell culture model used to predict iron bioavailability. Journal of Nutritional Biochemistry. 20(7):485-562.
Micronutrient Sprinkles Add More Bioavailable Iron to some Kenyan Complementary Foods: Studies Using an In Vitro Digestion/Caco-2 Cell Culture Model - (Peer Reviewed Journal)
Lung'Aho, M.G., Glahn, R.P. 2009. Micronutrient Sprinkles Add More Bioavailable Iron to some Kenyan Complementary Foods: Studies Using an In Vitro Digestion/Caco-2 Cell Culture Model. Maternal and Child Nutrition. 5 (2):151-158.
Development of a gastrointestinal tract microscale cell culture analog to predict drug transport - (Peer Reviewed Journal)
Mcauliffe, G.J., Chang, J., Glahn, R.P., Shuler, M.L. 2008. Development of a gastrointestinal tract microscale cell culture analog to predict drug transport. Molecular and Cellular Biomechanics. 5(2):119-132.
Iron bioavailability to piglets from red and white common beans (Phaseolus vulgaris L.) - (Peer Reviewed Journal)
Tan, S., Yeung, C., Glahn, R.P., Welch, R.M., Lei, X., Miller, D.D. 2008. Iron bioavailability to piglets from red and white common beans (Phaseolus vulgaris L.). Journal of Agriculture and Food Chemistry. 56(13):5008-14.
Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells - (Peer Reviewed Journal)
Laparra, J., Tako, E., Glahn, R.P., Miller, D. 2008. Inulin affects iron dialyzability from FeSO4 and FeEDTA solutions but does not alter Fe uptake by Caco-2 cells. Journal of Agricultural and Food Chemistry. 56:2846-2851.
Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based, probiotic-containing, yogurts but incubation at 37 oC does - (Peer Reviewed Journal)
Laparra, J., Tako, E., Glahn, R.P., Miller, D. 2008. Supplemental inulin does not enhance iron bioavailability to Caco-2 cells from milk- or soy-based, probiotic-containing, yogurts but incubation at 37 oC does. Food Chemistry. 109:122-128.
Genetic and biochemical analysis of iron bioavailability in maize - (Abstract Only)
Hoekenga, O., Mwankini, A., Buckler Iv, E.S., Glahn, R.P., Kochian, L.V. 2007. Genetic and biochemical analysis of iron bioavailability in maize. American Society of Agronomy Meetings. Volume 52:102.
Genetic and biochemical analysis of iron bioavailability in maize - (Abstract Only)
Hoekenga, O., Mwaniki, A., Buckler Iv, E.S., Glahn, R.P., Kochian, L.V. 2007. Genetic and biochemical analysis of iron bioavailability in maize (abstract). Agronomy Society of America, Crop Science Society of America, Soil Science Society of America Meeting. Session 176:4
Screening of iron bioavailability patterns in eight bean (Phaseolus vulgaris L.) genotypes using the Caco-2 cell in vitro model - (Peer Reviewed Journal)
Ariza-Nieto, M., Blair, M., Welch, R.M., Glahn, R.P. 2007. Screening of iron bioavailability patterns in eight bean (Phaseolus vulgaris L.) genotypes using the Caco-2 cell in vitro model. Journal of Agricultural and Food Chemistry. 55:7950-7956.
Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine - (Peer Reviewed Journal)
Tako, E., Glahn, R.P., Welch, R.M., Lei, X., Yasuda, K., Miller, D. 2008. Dietary inulin affects the expression of intestinal enterocyte iron transporters, receptors and storage protein and alters the microbiota in the pig intestine. British Journal of Nutrition. 99:472-480.
Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions Reprint Icon - (Peer Reviewed Journal)
Beisiegel, J.M., Hunt, J.R., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B. 2007. Iron bioavailability from maize and beans: a comparison of human measurements with Caco-2 cell and algorithm predictions. American Journal of Clinical Nutrition. 86:388-396.
A Comparison of Physical Properties, Screening Procedures and a Human Efficacy Trial for Predicting the Bioavailability of Commercial Elemental Iron Powders Used for Food Fortification Reprint Icon - (Peer Reviewed Journal)
Lynch, S.R., Bothwell, T., Campbell, L., Cowan, K., Glahn, R.P., Hallberg, L., Hoppe, M., Hulthen, L., Hunt, J.R., Hurrell, R.F., Miller, D., Swain, J., Turner, L., Winichagoon, P., Yeung, C.K., Zeder, C., Zimmermann, M.B. 2007. A comparison of physical properties, screening procedures and a human efficacy trial for predicting the bioavailability of commercial elemental iron powders used for food fortification. International Journal for Vitamin and Nutrition Research. 77:107-124.
Kaempferol in red and pinto bean seed (PHASEOLUS VULGARIS L.) coats inhibits iron bioavailability using an in vitro digestion/human CACO-2 cell model - (Peer Reviewed Journal)
Hu, Y., Cheng, Z., Heller, L., Krasnoff, S.B., Glahn, R.P., Welch, R.M. 2006. Kaempferol in red and pinto bean seed (PHASEOLUS VULGARIS L.) coats inhibits iron bioavailability using an in vitro digestion/human CACO-2 cell model. Journal of Agricultural and Food Chemistry. 54:9254-9261.
Moving toward a more physiological model: application of mucin to refine the in vitro digestion/Caco-2 cell culture system - (Peer Reviewed Journal)
Jin, F., Welch, R.M., Glahn, R.P. 2006. Moving toward a more physiological model: application of mucin to refine the in vitro digestion/Caco-2 cell culture system. Journal of Agricultural and Food Chemistry. 54:8962-8967.
Iron bioavailability from maize and beans: a comparison of human and Caco-2 cell measurements Reprint Icon - (Abstract Only)
Beisiegel, J.M., Hunt, J.R., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B. 2007. Iron bioavailability from maize and beans: a comparison of human and Caco-2 cell measurements [abstract]. Program Abstracts, Consequences and Control of Micronutrient Deficiencies: Science, Policy, and Programs - Defining the Issues. April 16-18, 2007. Istanbul, Turkey. p. 103.
Bread Baking Does Not Alter the Bioavailability of Hydrogen-Reduced Iron Powder Added to Refined Wheat Flour - (Peer Reviewed Journal)
Maekawa, A.A., Glahn, R.P., Lei, X., Miller, D. 2006. Bread baking does not alter the bioavailability of hydrogen-reduced iron powder added to refined wheat flour. Journal of Agricultural and Food Chemistry. 54:8362-8368.
Iron Dissociates from the Nafeedta Complex Prior to Or During Intestinal Absorption in Rats - (Peer Reviewed Journal)
Zhu, L., Yeung, C., Glahn, R.P., Miller, D. 2006. Iron dissociates from the nafeedta complex prior to or during intestinal absorption in rats. Journal of Agricultural and Food Chemistry. 54:7929-7934.
Iron Uptake by Caco-2 Cells from Nafeedta and Feso4: Effects of Ascorbic Acid, Ph, and a Fe(ii) Chelating Agent - (Review Article)
Zhu, L., Yeung, C., Miller, D., Glahn, R.P. 2006. Iron uptake by caco-2 cells from nafeedta and feso4: effects of ascorbic acid, ph, and a fe(ii) chelating agent. Journal of Agricultural and Food Chemistry. 54:7924-7928.
Iron (Fe) Bioavailability and the Distribution of Anti-Fe Nutrition Biochemicals in the Unpolished, Polished Grain and Bran Fraction of Five Rice Genotypes - (Peer Reviewed Journal)
Prom-U-Thai, C., Glahn, R.P., Welch, R.M., Huang, L., Rerkasem, B. 2006. Iron (fe) bioavailability and the distribution of anti-fe nutrition biochemicals in the unpolished, polished grain and bran fraction of five rice genotypes. Journal of the Science of Food and Agriculture. 86(8):1209-1215.
A Caco-2 Cell Model Predicts Relative Iron Absorption from Tropical Maize by Women - (Abstract Only)
Beisiegel, J.M., Glahn, R.P., Welch, R.M., Menkir, A., Maziya-Dixon, B.B., Hunt, J.R. 2006. A caco-2 cell model predicts relative iron absorption from tropical maize by women [abstract]. FASEB J. 20(4):A624.
Meat and Ascorbic Acid Can Promote Fe Availability from Fe-Phytate But Not from Fe-Tannic Acid Complexes - (Peer Reviewed Journal)
Engle-Stone, R., Yeung, A., Welch, R.M., Glahn, R.P. 2005. Meat and ascorbic acid can promote fe availability from fe-phytate but not from fe-tannic acid complexes. Journal of Agricultural and Food Chemistry. 53:10276-10284.
Endosperm Specific Co-Expression of Recombinant Soybean Ferritin and Aspergillus Phytase in Maize Results in Significant Increases in the Levels of Bioavailable Iron - (Peer Reviewed Journal)
Drakakaki, G., Marcel, S., Glahn, R.P., Lund, L., Periagh, S., Christou, P., Stoger, E. 2005. Endosperm specific co-expression of recombinant soybean ferritin and aspergillus phytase in maize results in significant increases in the levels of bioavailable iron. Plant Molecular Biology. 59(6):869-880.
The Usefulness of Invitro Models to Predict the Bioavailability of Iron and Zinc: a Consensus Statement from the Harvestplus Expert Consultation Reprint Icon - (Other)
Fairweather-Tait, S., Lynch, Sean, Hunt, J.R., et al. 2005. The usefulness of in vitro models to predict the bioavailability of iron and zinc: a consensus statement from the HarvestPlus expert consultation. International Journal for Vitamin and Nutrition Research.75(6):371-374.
Prebiotics and Iron Bioavailability - Is There a Connection?– - (Literature Review)
Yeung, C., Miller, D., Welch, R.M., Glahn, R.P. 2005. Prebiotics and iron bioavailability - is there a connection?–. Journal of Food Science. 70:1288-1292.
Bioavailability of Elemental Iron Powders in Bread Assessed with An in Vitro Digestion/caco-2 Cell Culture Model - (Peer Reviewed Journal)
Yeung, C., Miller, D.D., Zhiqiang, C., Glahn, R.P. 2005. Bioavailability of elemental iron powders in bread assessed with an in vitro digestion/caco-2 cell culture model. Journal of Food Science. 70:5199-5203.
Genotype-Environment Interactions and Stability Analyses of Kernel-Iron and -Zinc Concentrations in Tropical Maize - (Peer Reviewed Journal) - (04-Nov-04)
Inhibition of Iron Uptake from Iron Salts and Chelates by Divalent Metal Cations in Intestinal Epithelial Cells - (Peer Reviewed Journal)
Yeung, C.K., Glahn, R.P., Miller, D. 2004. Inhibition of iron uptake from iron salts and chelates by divalent metal cations in intestinal epithelial cells. Journal of Agricultural and Food Chemistry. Available: http://pubs.acs.org/journals/jafcau/.
Iron Availability of a Fortified Processed Wheat Cereal: a Comparison of 14 Fe Forms Using An in Vitro Digestion/caco-2 Model - (Peer Reviewed Journal)
Wortley, G., Leusner, S., Good, C., Gugger, E., Glahn, R.P. 2004. Iron availability of a fortified processed wheat cereal: a comparison of 14 fe forms using an in vitro digestion/caco-2 model. British Journal of Nutrition. 93:65-71.
Enhancing the Absorption of Fortification Iron: a Sustain Task Force Report - (Other)
Yeung, C., Miller, D., Hurrell, R.F., Lynch, S., Bothwell, T., Cori, H., Hertrampf, E., Kratky, Z., Rodenstein, M., Glahn, R.P. 2004. Enhancing the absorption of fortification iron: a sustain task force report. International Journal for Vitamin and Nutrition Research. 74:387-401.
The Effect of Calcium Salts, Ascorbic Acid and Peptic Ph on Calcium, Zinc and Iron Bioavailabilities from Fortified Human Milk - (Peer Reviewed Journal)
Etcheverry, P., Wallingford, J., Miller, D., Glahn, R.P. 2005. The effect of calcium salts, ascorbic acid and peptic ph on calcium, zinc and iron bioavailabilities from fortified human milk. International Journal for Vitamin and Nutrition Research. 75:171-178.
In Vitro Digestion/caco-2 Cell Model for Iron Bioavailability Shows Strong Correllation with Human Trials - (Peer Reviewed Journal)
Yun, S., Habicht, J., Miller, D.D., Glahn, R.P. 2004. In vitro digestion/caco-2 cell model for iron bioavailability shows strong correllation with human trials. Journal of Nutrition. 134:2717-2721.
Iron Absorption from Nafeedta Is Down-Regulated in Iron-Loaded Rats - (Peer Reviewed Journal)
Yeung, C.K., Zhu, L., Glahn, R.P., Miller, D. 2004. Iron absorption from nafeedta is down-regulated in iron-loaded rats. Journal of Nutrition. 134:2270-2274.
Calcium, Zinc and Iron Bioavailabilities from a Commercial Human Milk Fortifier: a Comparison Study - (Peer Reviewed Journal)
Etcheverry, P., Wallingford, J.C., Miller, D.D., Glahn, R.P. 2004. Calcium, zinc and iron bioavailabilities from a commercial human milk fortifier: a comparison study. Journal of Dairy Science. 87:3629-3637.
Caco-2 Cell Fe Uptake from Human Milk and Infant Formula - (Peer Reviewed Journal)
Etcheverry, P., Wissler, J., Wortley, G., Glahn, R.P. 2004. Caco-2 cell fe uptake from human milk and infant formula. Nutrition Research. 24(7):573-579.
Carbohydrate Fractions from Cooked Fish Promote Iron Uptake by Caco-2 Cells - (Peer Reviewed Journal)
Glahn, R.P., Huh, E., Hotchkiss, A.T., Brouillette, J.N. 2004. Carbohydrate fractions from cooked fish promote iron uptake by caco-2 cells. Journal of Nutrition. 134:1681-1689.
Assessment of Carotenoid Bioavailability of Whole Foods Using a Caco-2 Cell Culture Model Coupled with An in Vitro Digestion - (Peer Reviewed Journal)
Liu, C., Glahn, R.P., Liu, R.H. 2004. Assessment of carotenoid bioavailability of whole foods using a caco-2 cell culture model coupled with an in vitro digestion. Journal of Agricultural and Food Chemistry. 52:4330-4337.
Assessment of Iron Bioavailability from 20 Elite Late-Maturing Tropical Maize Varieties Using An in Vitro Digestion/caco-2 Cell Model - (Peer Reviewed Journal)
Oikeh, S.O., Menkir, A., Maziya-Dixon, B., Welch, R.M., Glahn, R.P. 2004. Assessment of iron bioavailability from 20 elite late-maturing tropical maize varieties using an in vitro digestion/caco-2 cell model. Journal of the Science of Food and Agriculture. 84:1202-1206.
A Low Molecular Weight Factor in Human Milk Whey Promotes Iron Uptake by Caco-2 Cells - (Peer Reviewed Journal)
Etcheverry, P., Miller, D.D., Glahn, R.P. 2004. A low molecular weight factor in human milk whey promotes iron uptake by caco-2 cells. Journal of Nutrition. 134(1):93-98.
Iron "biofortification" of Tropical Maize to Reduce Human Iron Defficiency in West Africa - (Peer Reviewed Journal)
OIKEH, S.O., MENKIR, A., MAZIYA-DIXON, B., WELCH, R.M., GLAHN, R.P. 2003. IRON "BIOFORTIFICATION" OF TROPICAL MAIZE TO REDUCE HUMAN IRON DEFFICIENCY IN WEST AFRICA. JOURNAL OF PLANT NUTRITION. 26:2307-2319.
Assessment of Concentrations of Trace Minerals and Bioavailable Iron in Grains of Early-Maturing Tropical Maize Varieties - (Peer Reviewed Journal)
OIKEH, S.O., MENKIR, A., MAZIYA-DIXON, B., WELCH, R.M., GLAHN, R.P. ASSESSMENT OF CONCENTRATIONS OF TRACE MINERALS AND BIOAVAILABLE IRON IN GRAINS OF EARLY-MATURING TROPICAL MAIZE VARIETIES. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2003. v. 51. p. 3688-3694.
Iron Bioavailability from Common Raisin-Containing Foods Assessed with An in Vitro Digestion/caco-2 Cell Culture Model: Effects of Raisins - (Peer Reviewed Journal)
Yeung, C.K., Glahn, R.P., Miller, D.D. 2003. Iron bioavailability from common raisin-containing foods assessed with an in vitro digestion/caco-2 cell culture model: effects of raisins. Journal of Food Science. 68(5):1866-1870.
Simultaneous Determination of 45calcium and 65zinc Uptake by Caco-2 Cells - (Peer Reviewed Journal)
Etcheverry, P., Wallingford, J.C., Miller, D.D., Glahn, R.P. 2002. Simultaneous determination of 45calcium and 65zinc uptake by caco-2 cells. Journal of Agricultural and Food Chemistry. 50:6287-6294.
Red Grape Juice Inhibits Iron Bioavailability: Application of An in Vitro Digestion/caco-2 Cell Model - (Peer Reviewed Journal)
BOATO, F., WORTLEY, G.M., LIU, R., GLAHN, R.P. RED GRAPE JUICE INHIBITS IRON BIOAVAILABILITY: APPLICATION OF AN IN VITRO DIGESTION/CACO-2 CELL MODEL. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2002.
In Vitro Iron Bioavailability and Antioxidant Activity of Raisins - (Peer Reviewed Journal)
Yeung, C.K., Glahn, R.P., Wu, X., Liu, R.H., Miller, D.D. 2003. In vitro iron bioavailability and antioxidant activity of raisins. Journal of Food Science. 68(2):701-705.
Effects of Iron Source on Iron Availability from Casein and Casein Phosphopeptides - (Peer Reviewed Journal) - (30-Apr-02)
A Comparison of Iron Bioavailability from 15 Rice Genotypes: Studies Usingan in Vitro Digestion/caco-2 Cell Culture Model - (Peer Reviewed Journal) - (21-Mar-02)
Inhibition of Iron Uptake by Phytic Acid, Tannic Acid and Zncl2: Studies Using An in Vitro Digestion/caco-2 Cell Model - (Peer Reviewed Journal) - (01-Nov-01)
Comparison of the Availability of Various Iron Fortificants in Bread and Milk Using An in Vitro Digestion/caco-2 Cell Culture Method - (Peer Reviewed Journal) - (01-Oct-01)
Dephosphorylation of Sodium Caseinate, Enzymatically Hydrolyzed Casein and Casein Phosphopeptides by Intestinal Alkaline Phosphatase; Implications Foriron Availability - (Peer Reviewed Journal) - (09-Jan-01)
A Comparison of Iron Availability from Commercial Iron Preparations Using An in Vitro Digestion/caco-2 Cell Culture Model - (Peer Reviewed Journal) - (09-Nov-99)
Spinach: Nitrate Analysis of An Advanced Life Support (Als) Crop Cultured under Als Candidate Artificial Light Sources - (Proceedings) - (01-Jul-99)
Application of An in Vitro Digestion/caco-2 Cell Culture Model to Determine Optimal Ascorbic Acid to Fe Ratio in Commercial Infant Rice Cereal - (Peer Reviewed Journal) - (31-May-99)
Caco-2 Cell Ferritin Formation Predicts Non-Radiolabeled Food Iron Availability in An in Vitro Digestion/caco-2 Cell Culture Model - (Peer Reviewed Journal) - (07-May-98)
Decreased Citrate Improves Iron Availability from Infant Formula: Application of An in Vitro Digestion/caco-2 Cell Culture Model - (Peer Reviewed Journal) - (25-Aug-97)
Iron Uptake Is Enhanced in Caco-2 Cell Monolayers by Cysteine and Cysteinyl-Glycine - (Peer Reviewed Journal) - (04-Dec-96)
Caco-2 Cell Ferrous Iron Uptake But Not Transfer Is Down-Regulated in Cells Grown in High Iron Serum-Free Medium - (Peer Reviewed Journal) - (16-Aug-96)
Caco-2 Cell Iron Uptake from Meat and Casein Digests Parallels in Vivo Studies: Use of a Novel in Vitro Method for Rapid Estimation of Iron Bioavailability - (Peer Reviewed Journal) - (11-Oct-95)
Bathophenanthrolene Disulfonic Acid and Sodium Dithionite Effectively Removes Surface Bound Iron from Caco-2 Cell Monolayers - (Peer Reviewed Journal) - (19-Jan-95)
ARS News Articles
Last Modified: 7/28/2014
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