Projects

ADDING VALUE TO BIOFUELS PRODUCTION SYSTEMS BASED ON PERENNIAL FORAGES Appropriated (D)   Accession Number: 418601

Determining Influence of Microbial, Feed, and Animal Factors on Efficiency of Nutrient Utilization and Performance in Lactating Dairy Cows Appropriated (D)   Accession Number: 422301

Removing Limitations to the Efficient Utilization of Alfalfa and Other Forages in Dairy Production, New Bio-Products, and Bioenergy to... Appropriated (D)   Accession Number: 422315

CONDUCTING DIGESTIBILITY TRIALS WITH LAMBS TO EVALUATE FORAGES Specific Cooperative Agreement (S)   Accession Number: 418773

FEEDING CANOLA MEAL TO LACTATING DAIRY COWS Specific Cooperative Agreement (S)   Accession Number: 420899

Amino Acid Nutrition of Lactating Dairy Cows Specific Cooperative Agreement (S)   Accession Number: 424312

EFFECT OF DIETARY PROTEIN CONTENT AND RUMEN-PROTECTED AMINO ACIDS ON NITROGEN UTILIZATION BY LACTATING DAIRY COWS Trust (T)   Accession Number: 422501

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Wet fractionation for improved utilization of alfalfa leaves - (Peer Reviewed Journal) - (20-Feb-13)

Proteomic analysis of two developmental stages of alfalfa-stem cell walls - (Peer Reviewed Journal)

Verdonk, J.C., Sullivan, M.L., Hatfield, R.D. 2012. Proteomic analysis of two developmental stages of alfalfa-stem cell walls. Frontiers in Plant Science. DOI: 10.3389/fpls.2012.00279.

Cell wall composition throughout development for the model grass Brachypodium distanchyon - (Peer Reviewed Journal)

Rancour, D.M., Marita, J.M., Hatfield, R.D. 2012. Cell wall composition throughout development for the model grass Brachypodium distanchyon. Frontiers in Plant Biotechnology. Available: doi: 10.3389/fpls.2012.00266.

Sodium hydroxide pretreatment of genetically modified switchgrass for improved enzymatic release of sugars - (Peer Reviewed Journal)

Wang, Z., Li, R., Xu, J., Marita, J.M., Hatfield, R.D., Qu, R., Cheng, J.J. 2012. Sodium hydroxide pretreatment of genetically modified switchgrass for improved enzymatic release of sugars. Bioresource Technology. 110:364-370.

Polyphenol oxidase activity in co-ensiled temperate grasses - (Abstract Only) - (02-Jan-12)

Co-ensiling temperate grasses to improve protein use efficiency in ruminants - (Proceedings)

Marita, J.M., Hatfield, R.D., Brink, G.E., Mertens, D.R. 2012. Co-ensiling temperate grasses to improve protein use efficiency in ruminants. In: Proceedings of the XVI International Silage Conference, July 2-4, 2012, Hameenlina, Finland. p. 132-133.

On-farm acidification and anaerobic storage for preservation and improved conversion of switchgrass into ethanol - (Peer Reviewed Journal)

Digman, M.F., Dien, B.S., Hatfield, R.D. 2012. On-farm acidification and anaerobic storage for preservation and improved conversion of switchgrass into ethanol. Biological Engineering (ASABE). 5(1):47-58.

Concentrations and apparent digestibility of lignin and carbohydrate fractions in cell walls of whole-crop cereal silages - (Abstract Only)

Wallsten, J., Hatfield, R.D. 2011. Concentrations and apparent digestibility of lignin and carbohydrate fractions in cell walls of whole-crop cereal silages. Journal of Dairy Science. 94(E-Supplement 1):233-234.

Incorporation of Monolignol Conjugates into Lignin for Improved Processing - (Abstract Only)

Ralph, J., Grabber, J.H., Hatfield, R.D., Lu, F., Kim, H., Marita, J.M., Wilkerson, C. 2010. Incorporation of Monolignol Conjugates into Lignin for Improved Processing. In: Proceedings of the XII Cell Wall Meeting, Porto, Portugal, 25-30 July 2010. Paper T12, p.8.

On-farm Preservation and Pretreatment of Perennial Grasses for Fuel Ethanol Production - (Abstract Only) - (21-May-10)

Enzymatic Processes Involved in the Incorporation of Hydroxycinnamates into Grass Cell Walls - (Review Article)

Hatfield, R.D., Marita, J.M. 2010. Enzymatic Processes Involved in the Incorporation of Hydroxycinnamates into Grass Cell Walls. Phytochemistry Reviews. 9:35-45.

Optimizing On-farm Pretreatment of Perennial Grasses for Fuel Ethanol Production - (Peer Reviewed Journal)

Digman, M.F., Shinners, K.J., Casler, M.D., Dien, B.S., Hatfield, R.D., Jung, H.G., Muck, R.E., Weimer, P.J. 2010. Optimizing On-farm Pretreatment of Perennial Grasses for Fuel Ethanol Production. Bioresource Technology. 101:5305-5314.

Reduced Lignin Alfalfa - Update - (Abstract Only)

Martin, N.P., Hatfield, R.D., Undersander, D.J. 2010. Reduced Lignin Alfalfa - Update. In: Proceedings of 2010 Symposium of the Midwest Forage Association, January 26-27, 2010, Wisconsin Dells, Wisconsin. Midwest Forage Association Presentations, Tuesday, 2.

In Vitro Proteolytic Inhibition, Polyphenol Oxidase Activity, and Soluble O-Diphenols in Grasses and Cereals - (Peer Reviewed Journal)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2010. In Vitro Proteolytic Inhibition, Polyphenol Oxidase Activity, and Soluble O-Diphenols in Grasses and Cereals. Journal of Agriculture and Food Chemistry. 58:959-966.

Progress report on reduced-lignin alfalfa: part 1, plant modifications - (Trade Journal)

Riday, H., Hatfield, R.D. 2009. Progress report on reduced-lignin alfalfa: part 1, plant modifications. Forage Focus. May. p. 2-3.

Incorporation of Monolignol Conjugates into Lignin for Improved Processing - (Abstract Only)

Ralph, J., Grabber, J.H., Lu, F., Kim, H., Marita, J.M., Hatfield, R.D. 2009. Incorporation of Monolignol Conjugates into Lignin for Improved Processing. Proceedings of IUFRO Rusts of Pines Conference. p. 11.

Comparing Corn Types for Differences in Cell Wall Characteristics and p-Coumaroylation of Lignin - (Peer Reviewed Journal)

Hatfield, R.D., Chaptman, A.K. 2009. Comparing Corn Types for Differences in Cell Wall Characteristics and p-Coumaroylation of Lignin. Journal of Agricultural and Food Chemistry. 57(10):4243-4249.

Grass Lignin Acylation: p-Coumaroyl Transferase Activity and Cell Wall Characteristics of C3 and C4 Grasses - (Peer Reviewed Journal)

Hatfield, R.D., Marita, J.M., Frost, K., Grabber, J.H., Ralph, J., Lu, F., Kim, H. 2009. Grass Lignin Acylation: p-Coumaroyl Transferase Activity and Cell Wall Characteristics of C3 and C4 Grasses. Planta. 229:1253-1267.

Incorporation of Monolignol Conjugates into Lignin for Improved Processing - (Proceedings)

Ralph, J., Grabber, J.H., Lu, F., Kim, H., Marita, J.M., Hatfield, R.D. 2009. Incorporation of Monolignol Conjugates into Lignin for Improved Processing. In: Proceedings of the Fifteenth International Symposium on Wood, Fiber, and Pulping Chemistry, June 15-18, 2009, Oslo, Norway. Paper O-014.

SOYBEAN STEM LIGNIN CONCENTRATION RELATES TO RESISTANCE TO SCLEROTINIA SCLEROTIORUM - (Peer Reviewed Journal)

Peltier, A.J., Hatfield, R.D., Grau, C.R. 2009. Soybean stem lignin concentration relates to resistance to Sclerotinia sclerotiorum. Plant Disease. 93(2):149-154.

CHARACTERIZATION OF P-COUMARATE ACCUMULATION, P-COUMAROYL TRANSFERASE, AND CELL WALL CHANGES DURING THE DEVELOPMENT OF CORN STEMS - (Peer Reviewed Journal)

Hatfield, R.D., Marita, J.M., Frost, K. 2008. Characterization of p-coumarate accumulation, p-coumaroyl transferase, and cell wall changes during the development of corn stems. Journal of the Science of Food and Agriculture. 88:2529-2537.

Coniferyl Ferulate Incorporation into Lignin Enhances the Alkaline Delignification and Enzymatic Degradation of Cell Walls - (Peer Reviewed Journal)

Grabber, J.H., Hatfield, R.D., Ralph, J., Lu, F. 2008. Coniferyl Ferulate Incorporation into Lignin Enhances the Alkaline Delignification and Enzymatic Degradation of Cell Walls. Biomacromolecules. 9:2510-2516.

A POTENTIAL ROLE FOR SINAPYL P-COUMARATE AS A RADICAL TRANSFER MECHANISM IN GRASS LIGNIN FORMATION - (Peer Reviewed Journal)

Hatfield, R.D., Ralph, J., Grabber, J.H. 2008. A potential role for sinapyl p-coumarate as a radical transfer mechanism in grass lignin formation. Planta. 228(6):919-928.

VARIABILITY OF GRASS LIGNIN ACYLATION - (Abstract Only)

Hatfield, R.D., Marita, J.M., Frost, K. 2008. Variability of grass lignin acylation [abstract]. The Pan American Congress on Plants and Bioenergy, June 22-25, 2008, Merida, Mexico. Abstract M705, p. 36.

A NOVEL RED CLOVER HYDROXYCINNAMOYL TRANSFERASE HAS ENZYMATIC ACTIVITIES CONSISTENT WITH A ROLE IN PHASALIC ACID [2-O-(CAFFEOYL)-L-MALATE] BIOSYNTHESIS - (Abstract Only)

Sullivan, M.L., Hatfield, R.D. 2008. A novel red clover hydroxycinnamoyl transferase has enzymatic activities consistent with a role in phasalic acid [2-o-(caffeoyl)-l-malate] biosynthesis [abstract]. Gordon Research Conference on Plant Molecular Biology, July 13-18, 2008, Holderness, New Hampshire.

CHARACTERISTICS OF POLYPHENOL OXIDASES - (Abstract Only)

Hatfield, R.D., Sullivan, M.L., Schmitz, G. 2008. Characteristics of polyphenol oxidases [abstract]. The 2008 Joint Meeting of the American Society of Plant Biologists and the Sociedad Mexicana de Bioquimica Rama: Bioquimica y Biologia Molecular de Plantas, June 26-July 1, 2008, Merida, Mexico. Abstract P04010. p. 103.

CHARACTERIZATION OF A p-COUMAROYL TRANSFERASE RESPONSIBLE FOR THE INCORPORATION OF pCA INTO GRASS CELL WALLS - (Abstract Only)

Hatfield, R.D., Marita, J.M. 2008. Characterization of a p-coumaroyl transferase responsible for the incorporation of pCA into grass cell walls [abstract]. Ferulate '08: An International Conference on Hydroxycinnamates and Related Plant Phenolics, August 25-27, 2008, Minneapolis, Minnesota. p. 53.

COPOLYMERIZATION OF CONIFERYL FERULATE WITH MONOLIGNOLS: IMPACT ON LIGNIN FORMATION AND ALKALINE DELIGNIFICATION OF MAIZE CELL WALLS - (Abstract Only)

Grabber, J. H., Ralph, J., Hatfield, R. D., Lu, F. 2008. Copolymerization of coniferyl ferulate with monolignols: impact on lignin formation and alkaline delignification of maize cell walls. Proceedings of Ferulate '08. p. 36.

Coniferyl ferulate incorporation into lignin dramatically enhances the delignification and enzymatic hydrolysis of maize cell walls - (Abstract Only)

Grabber, J. H., Hatfield, R. D., Ralph, J., Lu, F. 2008. Coniferyl ferulate incorporation into lignin dramatically enhances the delignification and enzymatic hydrolysis of maize cell walls [abstract]. Proceedings of Biotechnology for Fuels and Chemicals Symposium. 8(05):56.

ALFALFA: FORAGE CROP OF THE FUTURE - (Proceedings)

Martin, N.P., Mertens, D.R., Hatfield, R.D., Jung, H.J. 2008. Alfalfa: forage crop of the future. In: Proceedings of the 28th Kentucky Alfalfa Conference, February 21, 2008, Cave City, Kentucky, p. 17-26.

CLONING OF AN ALFALFA POLYPHENOL OXIDASE GENE AND EVALUATION OF ITS POTENTIAL IN PREVENTING POSTHARVEST PROTEIN DEGRADATION - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D., Samac, D.A. 2008. Cloning of an alfalfa polyphenol oxidase gene and evaluation of its potential in preventing postharvest protein degradation. Journal of the Science of Food and Agriculture. 88(8):1406-1414.

DESIGNING LIGNINS FOR IMPROVED BIOMASS PROCESSING - (Proceedings)

Ralph, J., Hatfield, R.D., Grabber, J.H., Lu, F., Kim, H., Marita, J.M. 2008. Designing lignins for improved biomass processing. In: Proceedings of the Funcfiber 2008 International Symposium on the Biology and Biotechnology of Wood, March 10-12, 2008, Umea, Sweden. p. 14

THREE POLYPHENOL OXIDASES FROM RED CLOVER (TRIFOLIUM PRATENSE) DIFFER IN ENZYMATIC ACTIVITIES AND ACTIVATION PROPERTIES - (Peer Reviewed Journal)

Schmitz, G.E., Sullivan, M.L., Hatfield, R.D. 2008. Three polyphenol oxidases from red clover (Trifolium pratense) differ in enzymatic activities and activation properties. Journal of Agricultural and Food Chemistry. 56(1):272-280.

CHARACTERISTICS OF POLYPHENOL OXIDASES FROM RED CLOVER (TRIFOLIUM PRATENSE) - (Abstract Only)

Schmitz, G.E., Hatfield, R.D., Sullivan, M.L. 2007. Characteristics of polyphenol oxidases from red clover (Trifolium pratense)[abstract]. In: Program and Abstract Book of the Plant Biology and Botany 2007 Joint Congress, July 8-11, 2007, Chicago, Illinois. p. 23.

LIGNIN ACYLATION IN GRASSES - (Abstract Only)

Marita, J.M., Hatfield, R.D., Frost, K.E. 2007. Lignin acylation in grasses [abstract]. In: Program and Abstract Book of the Plant Biology and Botany 2007 Joint Congress, July 8-11, 2007, Chicago, Illinois. p. 106.

Red clover polyphenol oxidase reduces ruminal lipolysis in in vitro batch culture - (Proceedings)

Lee, M.R., Minchin, F.R., Hatfield, R.D., Sullivan, M.L. 2007. Red clover polyphenol oxidase reduces ruminal lipolysis in in vitro batch culture. In: Proceedings of the British Society of Animal Science. April 2-4, 2007, Southport, United Kingdom. p. 25.

ON-FARM PRETREATMENT TECHNOLOGIES FOR IMPROVING ENZYMATIC DIGESTIBILITY OF CELLULOSE AND HEMICELLULOSE PRESENT IN PERENNIAL GRASS - (Proceedings)

Digman, M.F., Shinners, K.J., Dien, B.S., Hatfield, R.D., Li, X., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass. In: Proceedings of 2007 Annual Meeting of American Society of Agricultural and Biological Engineers, June 17, 2007, Minneapolis, Minnesota. Paper No. 071021.

NUTRITIONAL CHEMISTRY OF FORAGES - (Book / Chapter)

Hatfield, R.D., Jung, H.G., Broderick, G.A., Jenkins, T. 2007. Nutritional chemistry of forages. In: Barnes, R.F., Nelson, C.J., Moore, K.J., Collins, M., editors. The Science of Grassland Agriculture. Sixth Edition of Forages, Volume II. Ames, IA:Blackwell Publishing. p. 467-485.

CELL-WALL COMPOSITION OF SMOOTH BROMEGRASS PLANTS SELECTED FOR DIVERGENT FIBER CONCENTRATION - (Peer Reviewed Journal)

Casler, M.D., Hatfield, R.D. 2006. Cell-wall composition of smooth bromegrass plants selected for divergent fiber concentration. Journal of Agriculture and Food Chemistry. 54:8206-8211.

Transforming Forage Plants to Increase Nitrogen Utilization in Dairy Systems: What Are the Possibilities? - (Abstract Only)

Hatfield, R.D., Grabber, J.H., Sullivan, M.L., Waghorn, G., Mccaslin, M. 2006. Transforming Forage Plants to Increase Nitrogen Utilization in Dairy Systems: What Are the Possibilities? Journal of Dairy Science. 89(supplement 1):633.

MICROGRAVITY EFFECTS ON LEAF MORPHOLOGY, CELL STRUCTURE, CARBON METABOLISM AND MRNA EXPRESSION OF DWARF WHEAT - (Peer Reviewed Journal)

Stutte, G.W., Monje, O., Hatfield, R.D., Paul, A.-L., Ferl, R.J., Simone, C.G. 2006. Microgravity effects on leaf morphology, cell structure, carbon metabolism and mRNA expression of dwarf wheat. Planta. 224:1038-1049.

REINVENTING ALFALFA FOR DAIRY CATTLE AND NOVEL USES - (Proceedings)

Martin, N.P., Hatfield, R.D., Mertens, D.R. 2005. Reinventing alfalfa for dairy cattle and novel uses. Proceedings of the 35th California Alfalfa & Forage Symposium. p. 299-311.

POLYPHENOL OXIDASE AND O-DIPHENOLS INHIBIT POST-HARVEST PROTEOLYSIS IN RED CLOVER AND ALFALFA - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D. 2006. Polyphenol oxidase and o-diphenols inhibit post-harvest proteolysis in red clover and alfalfa. Crop Science. 46:662-670.

LIGNIN ACYLATION IN GRASSES - (Abstract Only)

Hatfield, R.H., Marita, J.M., Frost, K. 2005 Lignin acylation in grasses [abstract]. Cell Wall Biosynthesis Meeting, Paper No.78

POLYPHENOL OXIDASE ACTIVITY, O-DIPHENOLS, BROWNING, AND IN VITRO PROTEOLYTIC INHIBITION IN GRASSES - (Abstract Only)

Hatfield, R.D., Marita, J.M., Brink, G.E. 2005. Polyphenol oxidase activity, o-diphenols, browning, and in vitro proteolytic inhibition in grasses [abstract]. ASA-CSSA-SSSA International Annual Meetings. Paper No. 241-3.

DETERMINATION OF POLYPHENOL OXIDASE ACTIVITY (PPO) AND PROTEOLYTIC INHIBITION IN GRASSES - (Abstract Only)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Determination of polyphenol oxidase activity (PPO) and proteolytic inhibition in grasses [abstract]. Plant Biology. p. 210.

USING THE RED CLOVER POLYSHENOL OXIDASE GENE TO INHIBIT PROTEOLYTIC ACTIVITY IN ALFALFA - (Proceedings)

Hatfield, R.D., Sullivan, M.L. Muck, R.E. 2005. Using the red clover polyphenol oxidase gene to inhibit proteolytic activity in alfalfa. In: Park, R.S., Stronge, M.D., editors. Silage Production and Utilization. XIVth International Silage Conference, a Satellite Workshop of the XXth International Grassland Congress, July 2005, Belfast, Northern Ireland. p. 212.

USDA ARS NUTRIENT MANAGEMENT RESEARCH LABORATOY 50% SUBMISSION - (Abstract Only)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Polyphenol oxidase activity and in vitro proteolytic inhibition in grasses [abstract]. XIVth International Silage Conference. p. 210.

POTENTIAL BENEFITS OF PLANT MODIFICATION OF ALFALFA AND CORN SILAGE TO DAIRY DIETS - (Review Article)

Hartnell, G.F., Hatfield, R.D., Mertens, D.R., and Martin, N.P. 2005. Potential benefits of plant modification of alfalfa and corn silage to dairy diets. In: Proceedings of the 20th Southwest Nutrition and Management Conference, University of Arizona, Tucson. p. 156-172.

METHYL ESTERIFICATION DIVERGENTLY AFFECTS THE DEGRADABILITY OF PECTIC URONOSYLS IN NONLIGNIFIED AND LIGNIFIED MAIZE WALLS - (Peer Reviewed Journal)

Grabber, J.H. and R.D. Hatfield. 2005. Methyl Esterification Divergently Affects the Degradability of Pectic Uronosyls in Nonlignified and Lignified Maize Walls. Journal of Agriculture and Food Chemistry. 53: 1546-1549

POLYPHENOL OXIDASE ACTIVITY AND IN VITRO PROTEOLYTIC INHIBITION IN GRASSES - (Abstract Only)

Marita, J.M., Hatfield, R.D., Brink, G.E. 2005. Polyphenol oxidase activity and in vitro proteolytic inhibition in grasses. In: O'Mara, F.P., Wilkins, L., 't Mannetje, D.K., Lovett, R.A., Rogers, M., Boland, T.J., editors. XX International Grassland Congress: Offered Papers. Paper# 220.

CLONING AND CHARACTERIZATION OF RED CLOVER POLYPHENOL OXIDASE CDNAS AND EXPRESSION OF ACTIVE PROTEIN IN ESCHERICHIA COLI AND TRANSGENIC ALFALFA - (Peer Reviewed Journal)

Sullivan, M.L., Hatfield, R.D., Thoma, S., Samac, D.A. 2004. Cloning of red clover polyphenol oxidase cdnas and expression of active protein in escherichia coli and transgenic alfalfa. Plant Physiology. 136:3234-3244.

PEROXIDASE-DEPENDENT CROSS-LINKING REACTIONS OF P-HYDROXYCINNAMATES IN PLANT CELL WALLS - (Peer Reviewed Journal)

Ralph, J., Bunzel, M., Marita, J.M., Hatfield, R.D., Lu, F., Kim, H., Schatz, P.F., Grabber, J.H., Steinhart, H. 2004. Peroxidase-dependent cross-linking reactions of p-hydroxycinnamates in plant cell walls. Phytochemistry Reviews. 3:79-96

LIGNINS AND FERULATE-CONIFERYL ALCOHOL CROSS-COUPLING PRODUCTS IN CEREAL GRAINS - (Peer Reviewed Journal)

Bunzel, M., Ralph, J., Lu, F., Hatfield, R.D., Steinhart, H. 2004. Lignins and ferulate-coniferyl alcohol cross-coupling products in cereal grains. Journal of Agricultural and Food Chemistry. 52:6496-6502.

POLYPHENOL OXIDASE AND O-DIPHENOLS INHIBIT POST-HARVEST PROTEOLYSIS IN RED CLOVER AND ALFALFA - (Abstract Only)

Sullivan, M.L., Hatfield, R.D., Muck, R.E. Polyphenol oxidase and o-diphenols inhibit post-harvest proteolysis in red clover and alfalfa. 2004. North American Alfalfa Improvement Conference. Available: www.naaic.org/meeting/National/2004NAAIC8TC/2004abstracts/msullivan.pdf.

PROTEIN-BINDING POLYPHENOLS ENHANCE NITROGEN CYCLING AND PROFITABILITY OF DAIRY FARMS - (Proceedings)

Grabber, J.H., Broderick, G.A., Sullivan, M.L., Hatfield, R.D., Powell, J.M., Rotz, C.A., Muck, R.E., Ehlke, N.J. 2004. Protein-binding polyphenols enhance nitrogen cycling and profitability of dairy farms. In: 228th ACS National Meeting, August 22-26, 2004, Philadelphia, Pennsylvania. 2004 CDROM.

LIGNINS: NATURAL POLYMERS FROM OXIDATIVE COUPLING OF 4-HYDROXYPHENYLPROPANOIDS - (Peer Reviewed Journal)

Ralph, J., Lundquist, K., Brunow, G., Lu, F., Kim, H., Schatz, P.F., Marita, J.M., Hatfield, R.D., Ralph, S.A., Christensen, J.H., Boerjan, W. 2004. Lignins: natural polymers from oxidative coupling of 4-hydroxyphenylpropanoids. Phytochemistry Reviews 3(1):29-60.

POLYMERIZATION RATE AND PH EFFECTS ON LIGNIN FORMATION AND LIGNIN-MATRIX INTERACTIONS IN MAIZE WALLS - (Proceedings) - (12-Jun-03)

APOPLASTIC PH AND MONOLIGNOL ADDITION RATE EFFECTS ON LIGNIN FORMATION AND CELL WALL DEGRADABILITY IN MAIZE - (Peer Reviewed Journal)

Grabber, J.H., Hatfield, R.D., Ralph, J. 2003. Apoplastic ph and monolignol addition rate effects on lignin formation and cell wall degradability in maize. Journal of Agricultural and Food Chemistry. 51:4984-4989.

CLONING OF RED CLOVER AND ALFALFA POLYPHENOL OXIDASE GENES AND EXPRESSION OF ACTIVE ENZYMES IN TRANSGENIC ALFALFA - (Book / Chapter)

Sullivan, M.L., Thoma, S., Samac, D.A., Hatfield, R.D. 2004. Cloning of red clover and alfalfa polyphenol oxidase genes and expression of active enzymes in transgenic alfalfa. In: Hopkins, A., Wang, Z-Y., Mian, R., Sledge, M., Barker, R.W. editors. Molecular Breeding of Forage and Turf. Netherlands: Kluwer Academic Publishers. p. 189-195.

ALFALFA POLYPHENOL OXIDASE IS EXPRESSED IN FLOWERS AND DEVELOPING SEEDS - (Proceedings)

SULLIVAN,M.L., EVANS,M., THOMA,S., HATFIELD,R.D., ALFALFA POLYPHENOL OXIDASE IS EXPRESSED IN FLOWERS AND DEVELOPING SEEDS, MEETING ABSTRACT, 2003.

DETERMINING THE ROLE OF P-COUMARYL TRANSFERASE IN LIGNIFICATION OF GRASSES - (Abstract Only) - (28-Feb-03)

MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN COMT AND CCOAOMT - (Proceedings)

MARITA, J.M., RALPH, J., HATFIELD, R.D., GUO, D., CHEN, F., DIXON, R. MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN COMT AND CCOAOMT. INTERNATIONAL SYMPOSIUM ON WOOD AND PULPING CHEMISTRY. 2003.

CLONING OF RED CLOVER AND ALFALFA POLYPHENOL OXIDASE GENES AND EXPRESSION OF ACTIVE ENZYMES IN TRANSGENIC ALFALFA - (Proceedings)

Sullivan, M.L., Thoma, S., Samac, D., Hatfield, R.D. Cloning of red clover and alfalfa polyphenol oxidase genes and expression of active enzymes in transgenic alfalfa. Molecular Breeding of Forage and Turf Conference. 2003. p. 189-195

REDESIGNING ALFALFA FOR DAIRY CATTLE - (Proceedings)

Martin, N.P., Hatfield, R.D., Mertens, D.R. 2003. Redesigning alfalfa for dairy cattle. In: Land O Lakes Feed Division Leading Producer Conference, January 21, 2003, Wisconsin Dells, Wisconsin. p. 1-3.

NUCLEAR MAGNETIC RESONANCE SPECTRA OF TWO TYPES OF LIGNIN - (Peer Reviewed Journal)

FUKUSHIMA, R.S., HATFIELD, R.D. NUCLEAR MAGNETIC RESONANCE SPECTRA OF TWO TYPES OF LIGNIN. Pesquisa Agropecuaria Brasileira. 2003a. v. 38. p. 505-511.

PHENOLIC COMPOSITION OF DIOXANE LIGNINS AS DETERMINED BY NITROBENZENE OXIDATIVE REACTION - (Peer Reviewed Journal)

FUKUSHIMA, R.S., HATFIELD, R.D. PHENOLIC COMPOSITION OF DIOXANE LIGNINS AS DETERMINED BY NITROBENZENE OXIDATIVE REACTION. MEETING ABSTRACT. 2003b. v. 38. p. 373-378.

VARIATIONS IN THE CELL WALL COMPOSITION OF MAIZE BROWN MIDRIB MUTANTS - (Peer Reviewed Journal)

MARITA, J.M., VERMERRIS, W., RALPH, J., HATFIELD, R.D. VARIATIONS IN THE CELL WALL COMPOSITION OF MAIZE BROWN MIDRIB MUTANTS. Journal of Agriculture and Food Chemistry. 2003. v. 51. p. 1313-1321. v. 51. pp. 1313-1321.

MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWNREGULATED IN CAFFEIC ACID 3-0-METHYLTRANSFERASE AND CAFFEOYL COENZYME A 3-0-METHYLTRANSFERASE - (Abstract Only) - (12-Dec-02)

SINAPATE DEHYDRODIMERS AND SINAPALE-FERULATE HETERODIMERS IN CEREAL DIETARY FIBER - (Peer Reviewed Journal) - (19-Nov-02)

STRUCTURAL AND COMPOSITIONAL MODIFICATIONS IN LIGNIN OF TRANSGENIC ALFALFA DOWN-REGULATED IN CAFFEIC ACID 3-O-METHYLTRANSFERASE AND CAFFEOYL COA 3-O-METHYLTRANSFERASE - (Peer Reviewed Journal) - (09-Sep-02)

MODEL STUDIES OF FERULATE-CONIFERYL ALCOHOL CROSS PRODUCT FORMATION IN PRIMARY MAIZE WALLS: IMPLICATIONS FOR LIGNIFICATION IN GRASSES - (Peer Reviewed Journal) - (30-Jul-02)

CHANGES IN CARBOHYDRATE COORDINATED PARTITIONING AND CELL WALL REMODELING WITH STRESS-INDUCED PATHOGENESIS IN WHEAT SHEATHS - (Peer Reviewed Journal) - (28-Mar-02)

CHARACTERIZATION OF RED CLOVER POLYPHENOL OXIDASE - (Abstract Only) - (28-Feb-02)

ISOLATION AND CHARACTERIZATION OF POLYPHENOL OXIDASE GENES FROM ALFALFA - (Abstract Only) - (28-Feb-02)

CLONING OF RED CLOVER POLYPHENOL OXIDASE CDNAS AND CHARACTERIZATION OF ACTIVE PROTEIN EXPRESSED IN TRANSGENIC ALFALFA - (Abstract Only) - (28-Feb-02)

NMR PROOF THAT LIGNINS OF COMT-DEFICIENT TRANSGENIC AND MUTANT ANGIOSPERMS INCORPORATE 5-HYDROXCONIFERYL ALCOHOL - (Proceedings) - (17-Feb-02)

CHEMICAL COMPOSITION AND ENZYMATIC DEGRADABILITY OF XYLEM AND NON-XYLEM WALLS ISOLATED FROM ALFALFA INTERNODES - (Peer Reviewed Journal) - (11-Feb-02)

5-HYDROXYCONIFERYL ALCOHOL AS A MONOLIGNOL IN COMT-DEFICIENT ANGIOSPERMS - (Proceedings) - (11-Jun-01)

LIGNIN FORMATION IN PLANTS: THE DILEMMA OF LINKAGE SPECIFICITY - (Review Article) - (25-Apr-01)

EXTRACTION AND ISOLATION OF LIGNIN FOR UTILIZATION AS A STANDARD TO I DETERMINE LIGNIN CONCENTRATION USING THE ACETYL BROMIDE SPECTROPHOTOMETRIC METHOD - (Peer Reviewed Journal) - (05-Apr-01)

ELUCIDATION OF NEW STRUCTURES IN LIGNINS OF CAD- AND COMT- DEFICIENT PLANTS BY NMR - (Peer Reviewed Journal) - (16-Nov-00)

URONATE METHYL ESTERS AND BENZYL-URONATE CROSS-LINKS RESTRICT THE ENZYMATIC HYDROLYSIS OF PECTIN IN CELL WALLS - (Abstract Only) - (09-Nov-00)

CROSS-LINKING OF MAIZE WALLS BY FERULATE DIMERIZATION AND INCORPORATION INTO LIGNIN - (Peer Reviewed Journal) - (17-Oct-00)

DIFERULATES ANALYSIS: NEW DIFERULATES AND DISINAPATES IN INSOLUBLE CEREAL FIBER - (Peer Reviewed Journal) - (01-Oct-00)

SYRINGYL LIGNIN AND POOL SIZES OF SINAPIC ACID AND SINAPYL ALCOHOL IN ARABIDOPSIS RELATED TO HR SIGNAL TRANSDUCTION AND CELL DEATH RESPONSES - (Abstract Only) - (29-Aug-00)

THE ROLES OF FERULATES IN CROSS-LINKING CELL WALL POLYSACCHARIDES - (Abstract Only) - (24-Aug-00)

IDENTIFICATION AND PURIFICATION OF A BACTERIOCIN FROM THE RUMINAL CELLULOLYTIC BACTERIUM RUMINOCOCCUS ALBUS 7. - (Abstract Only) - (31-Jul-00)

FERULATES IN CORN SEED TISSUES - (Abstract Only) - (15-Jul-00)

DIFERULATES AS STRUCTURAL COMPONENTS IN SOLUBLE AND INSOLUBLE CEREAL DIETARY FIBRE - (Proceedings) - (04-Apr-00)

FERMENTATION OF A BACTERIAL CELLULOSE/XYLAN COMPOSITE BY MIXED RUMINAL MICROFLORA: IMPLICATIONS FOR THE ROLE OF POLYSACCHARIDE MATRIX INTERACTIONSIN PLANT CELL WALL BIODEGRADABILITY - (Peer Reviewed Journal) - (08-Mar-00)

LAX LEAF MAIZE: CELL WALL COMPOSITION AND NUTRITIONAL VALUE - (Peer Reviewed Journal) - (15-Jan-00)

CHEMICAL COMPOSITION AND DEGRADABILITY OF XYLEM AND NONXYLEM - (Abstract Only) - (04-Nov-99)

NMR CHARACTERIZATION OF LIGNINS IN ARABIDOPSIS ALTERED IN THE ACTIVITY OF FERULATE 5-HYDROXYLASE - (Peer Reviewed Journal) - (01-Sep-99)

CHARACTERIZING PROTEOLYTIC INHIBITION IN RED CLOVER SILAGE - (Abstract Only) - (07-Jul-99)

ARE LIGNINS OPTICALLY ACTIVE? - (Peer Reviewed Journal) - (03-May-99)

LIGNIN STRUCTURE IN LIGNIN-BIOSYNTHETIC-PATHWAY MUTANTS AND TRANSGENICS; NEW OPPORTUNITIES FOR ENGINEERING LIGNINS - (Proceedings) - (09-Dec-98)

ARE LIGNINS OPTICALLY ACTIVE? - (Peer Reviewed Journal) - (09-Dec-98)

ARE LIGNINS OPTICALLY ACTIVE? - (Proceedings) - (09-Dec-98)

YIELD, COMPOSITION AND DIGESTION OF BERMUDAGRASS: EFFECTS OF BERMUDAGRASS CULTIVAR...ON YIELD, IN VITRO AND IN SITU DIGESTION, NUTRIENT AND CELL WALLCOMPOSITION AND FEED INTAKE AND DIGESTION BY GROWING BEEF STEERS - (Peer Reviewed Journal) - (06-Dec-98)

COMPARISON OF TIFTON 85 AND COASTAL BERMUDAGRASS FOR YIELD, NUTRIENT PARAMETERS, INTAKE, AND DIGESTION BY GROWING BEEF STEERS - (Peer Reviewed Journal) - (11-Nov-98)

NEW OPPORTUNITIES FOR ENGINEERING LIGNINS? - (Abstract Only) - (05-Sep-98)

NMR CHARACTERIZATION OF ALTERED LIGNINS EXTRACTED FROM TOBACCO PLANTS DOWN-REGULATED FOR LIGNIFICATION ENZYMES CINNAMYL-ALCOHOL DEHYDROGENASE ANDCINNAMOYL-COA-REDUCTASE - (Peer Reviewed Journal) - (30-Aug-98)

SOLUTION-STATE NMR OF LIGNINS - (Book / Chapter) - (27-Aug-98)

CELL WALL CROSS-LINKING BY FERULATES AND DEHYDRODIFERULATES IN GRASSES - (Review Article) - (24-Aug-98)

MODELING THE FEASIBILITY OF INTRAMOLECULAR DEHYDRODIFERULATE FORMATION IN GRASS WALLS - (Peer Reviewed Journal) - (24-Aug-98)

COMPOSITION OF CELL WALLS ISOLATED FROM CELL TYPES OF GRAIN SORGHUM STEMS - (Peer Reviewed Journal) - (21-Aug-98)

EFFECT OF ENZYME OR MICROBIAL TREATMENT OF BERMUDAGRASS FORAGES BEFORE ENSILING ON NUTRIENT COMPOSITION, CELL WALL CHEMISTRY AND IN SITU DIGESTIONKINETICS - (Peer Reviewed Journal) - (19-Aug-98)

CELL WALL STRUCTURAL FOUNDATIONS: MOLECULAR BASIS FOR IMPROVING FORAGE DIGESTIBILITIES - (Review Article) - (01-Aug-98)

MODELING CELL WALL COMPONENT INTERACTIONS - (Abstract Only) - (21-Jul-98)

MODELING THE FEASIBILITY OF INTRAMOLECULAR DEHYDRODIFERULATE FORMATION IN GRASS WALLS - (Abstract Only) - (11-Jul-98)

ROLE OF FERULATES IN CELL WALL CROSS-LINKING, LIGNIFICATION, AND DEGRADATION - (Proceedings) - (11-Jul-98)

CELL WALL MODELING, PEROXIDASE EXPRESSION AND APOPLASTIC LEVELS OF CARBOHYDRATES IN A PATHOGENIC ASSOCIATION WITH A FUNGAL ENDOPHYTE IN WHEAT SHEATHS - (Abstract Only) - (01-Jul-98)

MAKING BETTER SILAGE: FOLLOWING THE CLUES IN RED CLOVER - (Abstract Only) - (12-Jun-98)

FERULATE CROSS-LINKS LIMIT THE ENZYMATIC DEGRADATION OF SYNTHETICALLY LIGNIFIED PRIMARY WALLS OF MAIZE - (Peer Reviewed Journal) - (06-May-98)

NEW OPPORTUNITIES FOR ENGINEERING LIGNINS - (Abstract Only) - (05-May-98)

CELL WALL CROSS-LINKING IN GRASSES BY FERULATES AND DEHYDRODIFERULATES - (Proceedings) - (01-Apr-98)

SEVERE INHIBITION OF MAIZE WALL DEGRADATION BY SYNTHETIC LIGNINS FORMED WITH CONIFERALDEHYDE - (Peer Reviewed Journal) - (06-Jan-98)

UTILIZING THE ACETYL BROMIDE ASSAY TO DETERMINE LIGNIN CONCENTRATIONS IN HERBACEOUS PLANTS: SOME CAUTIONARY NOTES - (Peer Reviewed Journal) - (03-Dec-97)

THE MOLECULAR BASIS OF FORAGE QUALITY: STRUCTURAL FOUNDATION FOR IMPROVEMENT STRATEGIES - (Abstract Only) - (30-Oct-97)

MOLECULAR BASIS FOR FORAGE QUALITY: CELL WALL STRUCTURAL FOUNDATIONS FOR IMPROVEMENT STRATEGIES - (Abstract Only) - (24-Oct-97)

DIFERULATE CROSS-LINKS IMPEDE THE HYDROLYSIS OF NONLIGNIFIED MAIZE WALLS BY FUNGAL ENZYMES - (Peer Reviewed Journal) - (10-Sep-97)

FERULOYLATED ARABINOXYLANS AS INTERMEDIARIES IN CELL WALL CROSS-LINKING AND LIGNIN FORMATION IN GRASSES - (Abstract Only) - (22-Aug-97)

A POTENTIAL ROLE OF SINAPYL P-COUMARATE IN GRASS LIGNIN FORMATION - (Abstract Only) - (02-Aug-97)

FURTHER CHARACTERIZATION OF LIGNIFICATION IN TRACHEARY ELEMENTS DIFFERENTIATING IN CULTURE - (Abstract Only) - (02-Aug-97)

USING THE ACETYL BROMIDE ASSAY TO DETERMINE LIGNIN CONCENTRATIONS IN HERBACEOUS PLANTS: SOME CAUTIONARY NOTES - (Abstract Only) - (09-Jun-97)

ABNORMAL LIGNIN IN A LOBLOLLLY PINE MUTANT - (Peer Reviewed Journal) - (27-May-97)

FERULATES AND DIFERULATES AS NUCLEATION SITES FOR LIGNIFICATION IN GRASSES - (Peer Reviewed Journal) - (07-May-97)

MOLECULAR BASIS OF FORAGE QUALITY: CELL WALL STRUCTURAL FOUNDATIONS FOR IMPROVEMENT STRATEGIES - (Review Article) - (17-Apr-97)

FERULATE CROSS-LINKING IN GRASSES: CHEMISTRY, IMPACT ON DEGRADABILITY, ANDGENETIC MANIPULATION - (Abstract Only) - (10-Apr-97)

P-HYDROXYPHENYL, GUAIACYL, AND SYRINGYL LIGNINS HAVE SIMILAR INHIBITORY EFFECTS ON WALL DEGRADABILITY - (Peer Reviewed Journal) - (07-Apr-97)

CELL WALL CROSS-LINKING IN GRASSES BY FERULATES AND DIFERULATES - (Book / Chapter) - (18-Feb-97)

MODELING LIGNIFICATION IN GRASSES WITH DEHYDROGENATION POLYMER-CELL WALL COMPLEXES - (Book / Chapter) - (23-Jan-97)

KENAF'S UNUSUAL LIGNIN - (Abstract Only) - (13-Jan-97)

EXCRETION OF PURINE DERIVATIVES BY HOLSTEIN COWS ABOMASALLY INFUSED WITH INCREMENTAL AMOUNTS OF PURINES - (Peer Reviewed Journal) - (06-Dec-96)

IMPROVING FORAGE FIBER DIGESTIBILITY - (Trade Journal) - (01-Nov-96)

STRUCTURAL AND CHEMICAL CHANGES OF CELL WALL TYPES DURING STEM DEVELOPMENT:CONSEQUENCES FOR FIBER DEGRADATION BY RUMEN MICROFLORA - (Review Article) - (18-Sep-96)

IMPROVING ALFALFA UTILIZATION: BIOCHEMICAL STRATEGIES FOR IMPROVING ALFALFA UTILIZATION IN DAIRY PRODUCTION - (Proceedings) - (16-Sep-96)

SDS SOLUBILIZED LIGNIN COMPLEXES FROM ALFALFA STEMS - (Abstract Only) - (31-Jul-96)

MOLECULAR MODELLING OF DEHYDRODIFERULATES:ARE INTRAMOLECULAR DIMERS FEASIBLE WITHIN GRASS WALLS? - (Abstract Only) - (28-Mar-96)

ALTERING LIGNIN COMPOSITION, STRUCTURE AND CROSS-LINKING: POTENTIAL IMPACT ON CELL WALL DEGRADATION - (Abstract Only) - (28-Mar-96)

MECHANISMS OF POLYSACCHARIDE-POLYSACCHARIDE AND POLYSACCHARIDE-LIGNIN CROSS-LINKING IN GRASSES - (Abstract Only) - (28-Mar-96)

DEHYDROGENATION POLYMER-CELL WALL COMPLEXES AS A MODEL FOR LIGNIFIED GRASS WALLS - (Abstract Only) - (28-Mar-96)

MODELLING CROSS-LINKS WITHIN WALL MATRICES:IMPACT UPON POLYSACCHARIDE DEGRADATION - (Abstract Only) - (21-Mar-96)

CELL WALL CROSS-LINKING IN GRASSES BY FERULATES AND DEHYDRODIFERULATES - (Abstract Only) - (21-Mar-96)

THE ANTI-CELLULOLYTIC FACTOR FROM CICER MILKVETCH IS AN ARABINOGALACTAN PROTEIN - (Abstract Only) - (16-Nov-95)

SEVERE INHIBITION OF FIBER DEGRADATION BY CINNAMYL ALDEHYDE CONTAINING LIGNINS - (Abstract Only) - (03-Nov-95)

SEVERE INHIBITION OF FIBER DEGRADATION BY CINNAMYL ALDEHYDE CONTAINING LIGNINS - (Abstract Only) - (03-Oct-95)

DEHYDROGENATION POLYMER-CELL WALL COMPLEXES AS A MODEL FOR LIGNIFIED GRASS WALLS - (Peer Reviewed Journal) - (03-Oct-95)

FORMATION OF DEHYDRODIFERULES BY PEROXIDASES - (Abstract Only) - (29-Aug-95)

ANALYSIS OF FORAGE CROPS FOR PECTIN AND NONSTRUCTURAL CARBOHYDRATES - (Proceedings) - (15-Aug-95)

HEMICELLULOSES - (Book / Chapter) - (15-Aug-95)

PECTIC POLYSACCHARIDES IN CELL WALLS OF FORAGE LEGUMES - (Abstract Only) - (26-Jun-95)

LIGNIN-FERULATE CROSS-LINKS IN GRASSES. PART 3. ACTIVE INCORPORATION OF FERULATE POLYSACCHARIDE ESTERS INTO RYEGRASS LIGNINS - (Peer Reviewed Journal) - (13-Jun-95)

FERULATE CROSS-LINKING IN CELL WALLS ISOLATED FROM MAIZE CELL SUSPENSIONS - (Peer Reviewed Journal) - (09-Jun-95)

FERULATE CROSS-LINKING LIMITS DEGRADATION OF LIGNIFIED GRASS WALLS BY FUNGAL HYDROLASES - (Abstract Only) - (01-May-95)