Robert A Kunkle
Virus and Prion Research Unit
Veterinary Medical Science
Phone: (515) 663-7740 ext. 7740
Fax: (515) 663-7255
2300 Dayton Avenue
AMES, IA, 50010-0070
Di Bari, M.A., Nonno, R., Castilla, J., D'Agostino, C., Pirisinu, L., Riccardi, G., Conte, M., Richt, J., Kunkle, R., Langeveld, J., Vaccari, G., Agrimi, U. 2013. Chronic wasting disease in bank voles: characterisation of the shortest incubation time model for prion diseases. PLoS Pathogens. 9(3):e1003219.
Greenlee, J.J., Nicholson, E.M., Smith, J.D., Kunkle, R.A., Hamir, A.N. 2012. Susceptibility of cattle to the agent of chronic wasting disease from elk after intracranial inoculation. Journal of Veterinary Diagnostic Investigation. 24(6):1087-1093.
Sharma, V.K., Sacco, R.E., Kunkle, R.A., Bearson, S.M., Palmquist, D.E. 2012. Correlating levels of type III secretion and secreted proteins with fecal shedding of Escherichia coli O157:H7 in cattle. Infection and Immunity. 80(4):1333-1342.
Greenlee, J.J., Zhang, Xia, Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2012. Prolonged incubation time in sheep with prion protein containing lysine at position 171. Journal of Veterinary Diagnostic Investigation. 24(3):554-558.
Greenlee, J.J., Smith, J.D., Kunkle, R.A. 2010. White-tailed deer susceptible to scrapie by natural route of infection [abstract]. American College of Veterinary Pathologists. Paper No. 81.
Vargas, F., Kuroiwa, Y., Hamir, A.N., Greenlee, J.J., Kunkle, R.A., Kangas, T., Kanthasamy, A., Robl, J., Richt, J. A. 2010. Clinical features in prion protein-deficient and wild-type cattle inoculated with transmissible mink encephalopathy (TME) [abstract]. XXVI World Buiatrics Congress 2010, November 14-18, 2010, Santiago, Chile. Available: http://www.kenes.com/buiatrics/cd/pdf/780.pdf.
Hamir, A.N., Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Richt, J.A., Miller, J.M., Hall, M. 2011. Experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer by intracerebral route: final report. Canadian Journal of Veterinary Research. 75(2):152-156.
Loiacono, C.M., Beckwith, N., Kunkle, R.A., Orcutt, D., Hall, S.M. 2010. Detection of PrPSc in formalin-fixed, paraffin embedded tissue by Western blot differentiates classical scrapie, Nor98 scrapie, and bovine spongiform encephalopathy. Journal of Veterinary Diagnostic Investigation. 22(5):684-689.
Kunkle, R.A., Hamir, A.N., Richt, J., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2009. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-affected Sheep with the AA136, QR171 Prion Protein Genotype [abstract]. American Association of Veterinary Laboratory Diagnosticians. Paper No. 186.
Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Hamir, A.N. Susceptibility of Cattle to First-Passage Intracerebral Inoculation with Chronic Wasting Disease Agent from Elk [abstract]. American College of Veterinary Pathologists Annual Meeting. Veterinary Pathology. 46(5):1058.
Hamir, A.N., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2009. Experimental Oral Transmission of United States Origin Scrapie to Neonatal Sheep. Journal of Veterinary Diagnostic Investigation. 21(1):64-68.
Greenlee, J.J., Kunkle, R.A., Nicholson, E.M., Hamir, A.N., Bulgin, M.S., Richt, J. 2009. Strain Typing of U.S. Scrapie Strains Using a Panel of Inbred Mice [abstract]. American College of Veterinary Pathologists. Paper No. 121. p. 762.
Hamir, A.N., Palmer, M.V., Kunkle, R.A. 2008. Wasting and Neurologic Signs in a White-Tailed Deer (Odocoileus virginianus) Not Associated with Abnormal Prion Protein. Journal of Wildlife Diseases. 44(4):1045-1050.
Register, K.B., Kunkle, R.A. 2008. Strain-specific virulence of Bordetella hinzii in turkeys [abstract]. American Society for Microbiology Annual Meeting. Poster No. Z-023.
Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Miller, J.M., Hall, S.M., Schonenbrucher, H., Brunelle, B.W., Richt, J.A. 2007. Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer (Dama dama) [abstract]. European Society of Veterinary Pathology 25th Annual Meeting. Paper No. 49. p. 104.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2007. Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians 13th International Symposium, November 11-14, 2007, Melbourne, Australia. p. 29.
Hamir, A.N., Kunkle, R.A., Bulgin, M.S., Rohwer, R.G., Gregori, L., Richt, J.A. 2008. Experimental transmission of scrapie agent to susceptible sheep by intralingual and intracerebral inoculation. Canadian Journal of Veterinary Research. 72:63-67.
Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Miller, J.M., Hall, S.M., Schonenbrucher, H.H., Richt, J.A. 2007. Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deet to fallow deer [abstract]. Prion 2007. Paper No. PO2.43.
Nicholson, E.M., Kunkle, R.A., Hamir, A.N., Lebepe Mazur, S., Orcutt, D. 2007. Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot. Journal of Veterinary Diagnostic Investigation. 19:548-552.
Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J. 2007. Cross-species transmission of TSE. In: Proceedings of the American College of Veterinary Internal Medicine. 2007 ACVIM Forum, June 6-9, 2007, Seattle, Washington. Paper No. 160.
Hamir, A.N., Kunkle, R.A., Cutlip, R.C., Miller, J.M., Williams, E.S., Richt, J.A. 2006. Transmission of chronic wasting disease of mule deer to Suffolk sheep following intracerebral inoculation [abstract]. Conference of Research Workers in Animal Diseases 87th Annual Meeting. Paper No. P34. p. 108.
Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2006. Detection of PrP**Sc in formalin-fixed tissues by western blot [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 16.
Richt, J.A., Kunkle, R.A., Alt, D., Nicholson, E.M., Hamir, A.N., Czub, S., Kluge, J., Davis, A.J., Hall, S.M. 2007. Identification and characterization of two bovine spongiform encephalopathy cases diagnosed in the United States. Journal of Veterinary Diagnostic Investigation. 19(2):142-154.
Nicholson, E.M., Hamir, A.N., Kunkle, R.A. 2006. Detection of PrP**sc in formalin-fixed tissues by Western blot [abstract]. United States Animal Health Association. p. 332.
Hamir, A., Kunkle, R., Cutlip, R., Miller, J., Williams, E., Richt, J. 2006. Transmission of chronic wasting disease agent of mule deer (CWD**md) to Suffolk sheep by intracerebral route [abstract]. European Society of Veterinary Pathology 24th Annual Meeting. Paper No. P63. p. 171-172.
Greenlee, J.J., Kunkle, R.A. Nicholson, E.M., Lager, K.M., Hamir, A.N. 2006. Attempted transmission of scrapie and CWD to swine: preliminary findings. 19th International Pig Veterinary Society Congress. p. 386.
Hamir, A.N., Kunkle, R.A., Miller, J.M., Greenlee, J.J., Richt, J.A. 2006. Experimental transmission of transmissible mink encephalopathy (TME) to cattle by intracerebral inoculation [abstract]. 4th International Veterinary Vaccines and Diagnostics Conference. p. 89. Paper No. PO53.
Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2005. Experimental intracerebral and oral inoculation of scrapie to swine: preliminary report [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians 48th Annual Conference. p. 38.
Hamir, A.N., Kunkle, R.A., Miller, J.M., Hall, S.M. 2006. Abnormal prion protein in ectopic lymphoid tissue in a kidney of an asymptomatic white-tailed deer experimentally inoculated with the agent of chronic wasting disease. Veterinary Pathology. 43(3):367-369.
Kunkle, R.A., Wilson, M.A. 2008. Fowl Cholera. In: OIE Biological Standards Commission, editor. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2008: Mammals, Birds and Bees. 6th edition. Paris: Office International Des Epizooties. Chapter 2.3.9. p. 524-530.
Bessen, R.A., Dejoia, C., Dlakic, W., Sorg, R., O’Connell, K., Tucker, T., Kunkle, R.A., Hamir, A.N., Richt, J.A. 2005. Prion infection of mucosal tissue [abstract]. TSE Forum, Prion 2005: Between Fundamentals and Society's Needs. p. 31.
Hamir, A.N., Kunkle, R.A., Miller, J.M., Bartz, J.C., Richt, J.A. 2006. First and second cattle passage of transmissible mink encephalopathy (TME) by intracerebral inoculation. Veterinary Pathology. 43(2):118-126.
Hamir, A.N., Kunkle, R.A., Miller, J.M., Bartz, J.C., Richt, J.A. 2005. Experimental transmission of transmissible mink encephalopathy (TME) to cattle by intracerebral inoculation [abstract]. American College of Veterinary Pathologists Meeting. 42:706.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSE) at the National Animal Disease Center, Ames, Iowa: an update [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 169.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A., Bartz, J. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSEs) at the National Animal Disease Center (NADC), Ames, Iowa, USA: An update [abstract]. Annual Wildlife Disease Association Conference. p. 263.
Loynachan, A.T., Pettigrew, J.E., Wiseman, B.S., Kunkle, R.A., Harris, D.L. 2005. Evaluation of a diet free of animal protein in germfree swine. Xenotransplantation. 12:149-155.
Richt, J.A., Kluge, J.P., Alt, D.P., Kunkle, R.A., Hamir, A.N., Czub, S., Davis, A.J., Hall, S.M. 2004. Characterization of the recent U.S. BSE case and methods for surveillance. In: Proceedings of the 108th Annual Meeting of the United States Animal Health Association, October 22-27, 2004, Greensboro, North Carolina. p. 91-92.
Hamir, A.N., Kunkle, R.A., Richt, J.A., Cutlip, R.C., Miller, J.M., Orourke, K.I., Williams, E.S., Miller, M.W., Stack, M.J., Chaplin, M.J. 2004. Experimental transmission of chronic wasting disease agent to cattle by intracerebral route: final outcome of the study [abstract]. Animal Prion Diseases and the Americas. p. 78.
Richt, J.A., Kluge, J.P., Alt, D.P., Kunkle, R.A., Hamir, A.N., Czub, S., Davis, A.J., Hall, S.M. 2004. Identification and characterization of the U.S. bovine spongiform encephalopathy case [abstract]. Animal Prion Diseases and the Americas. p. 38.
Hamir, A.N., Kunkle, R.A., Richt, J.A., Cutlip, R.C., Miller, J.M., O'Rourke, K.I., Williams, E.S., Miller, M.W., Stack, M.J., Chaplin, M.J. 2004. Experimental transmission of chronic wasting disease agent to cattle by intracerebral route: final outcome of the study [abstract]. Annual Conference of the American College of Veterinary Pathologists. 41:588.
Hamir, A.N., Kunkle, R.A., Richt, J.A., Miller, J.M., Cutlip, R.C., Jenny, A.L. 2005. Experimental transmission of sheep scrapie by intracerebral and oral routes to genetically susceptible Suffolk sheep in the United States. Journal of Veterinary Diagnostic Investigation. 17(1):3-9.
Richt, J.A., Kluge, J.P., Alt, D.P., Kunkle, R.A., Hamir, A.N., Czub, S., Davis, A.J., Hall, S.M. 2004. Identification and characterization of the bovine spongiform encephalopathy case diagnosed in the United States [Abstract]. XXth International Winter Meeting of the Swiss Society of Neuropathology. Paper No. 13, p. 29.
Kunkle, R.A. Fowl cholera (avian pasteurellosis). Office International Epizootics Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 5th Edition. 2004. Chapt. 2.7.1.1 p. 921-926.
Richt, J.A., Kluge, J.P., Alt, D.P., Kunkle, R.A., Hamir, A.N., Czub, S., Davis, A.J., Hall, S. 2004. Identification and characterization of the first U.S. bovine spongiform encephalopathy case. Poster created for use by the USDA at a meeting with the Office of International Epizootics.
Hamir, A.N., Miller, J.M., Cutlip, R.C., Richt, J.A., Kunkle, R.A., O'Rourke, K.I., Jenny, A.L., Stack, M.J., Chaplin, M.J. 2003. Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation. 46th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. p. 210.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M.J., Chaplin, M.J., Miller, M., O'Rourke, K.I. 2003. Experimental cross-species transmission of chronic wasting disease (CWD-mule deer) to domestic livestock at the National Animal Disease Center: An update [Abstract]. 46th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians. p. 209.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M., Miller, M.W., O'Rourke, K.I., Chaplin, M.J. 2003. Experimental cross-species transmission of chronic wasting disease to domestic livestock at the National Animal Disease Center: An update [Abstract]. International Conference on Emerging Zoonoses. p. 91.
Hamir, A.N., Cutlip, R.C., Miller, J.M., Richt, J.A., Kunkle, R.A., Williams, E., Stack, M., Chaplin, M., Miller, M., O'Rourke, K.I. 2003. Experimental cross-species transmission of chronic wasting disease (CWD-mule deer) to domestic livestock at the National Animal Disease Center: An update [Abstract]. 52nd Annual Wildlife Disease Association Conference. p. 134.
HAMIR, A.N., CUTLIP, R.C., MILLER, J.M., RICHT, J., KUNKLE, R.A., WILLIAMS, E., STACK, M., CHAPLIN, M., MILLER, M., OROURKE, K.I. EXPERIMENTAL CROSS-SPECIES TRANSMISSION OF CHRONIC WASTING DISEASE (CWD-MULE DEER) TO DOMESTIC LIVESTOCK AT THE NATIONAL ANIMAL DISEASE CENTER: AN UPDATE. DISEASES AT THE INTERFACE BETWEEN DOMESTIC LIVESTOCK AND WILDLIFE SPECIES. 2003. ABSTRACT NO. 8.
Hamir, A.N., Miller, J.M., Cutlip, R.C., Kunkle, R.A., Jenny, A.L., Stack, M.J., Chaplin, M.J., Richt, J.A. 2004. Transmission of sheep scrapie to elk (Cervus elaphus nelsoni) by intracerebral inoculation: Final outcome of the experiment. Journal of Veterinary Diagnostic Investigation. 16(4):316-321.