James E Wells
(Jim)
Meat Safety & Quality Research
Research Microbiologist
Phone: (402) 762-4174
Fax: (402) 762-4209
U.S. MEAT ANIMAL RESEARCH CENTER
STATE SPUR 18D CLAY CENTER, NE, 68933
Durso, L.M., Wells, J., Kim, M.S. 2013. Diversity of microbiomes in beef cattle. In: Nelson, K., editor. Encyclopedia of Metagenomics. Springer Reference (www.springerreference.com). Springer-Verlag Berlin Heidelberg. Available: http://www.springerreference.com/docs/html/chapterdbid/333718.html.
Wells, J., Kalchayanand, N., Berry, E.D., Oliver, W.T. 2013. Effects of antimicrobials fed as dietary growth promoters on faecal shedding of Campylobacter, Salmonella and shiga-toxin producing Escherichia coli in swine. Journal of Applied Microbiology. 114(2):318-328.
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Wells, J., Wheeler, T.L. 2013. Chromogenic agar medium for detection and isolation of Escherichia coli serogroups O26,O45,O103,O111,O121, and O145 from fresh beef and cattle feces. Journal of Food Protection. 76(2):192-199.
Freetly, H.C., Hales, K.E., Wells, J. 2012. Rumen and cecum methane emissions between steers that are either negative or positive for residual gain [abstract]. Journal of Animal Science. 90(Suppl. 3):478.
Salter, R., Jones, S., Goldsmith, T., Quintana, J., Rapnicki, P., Shuck, K., Wells, J., Griffin, D. 2012. Development and model testing of anti-mortem screening methodology to predict prescribed drug withholds in heifers. In: Proceedings of International Association for Food Protection, July 22-25, 2012, Providence, Rhode Island. Poster P3-13.
Puchala, R., Animut, G., Patra, A.K., Detweiler, G.D., Wells, J., Varel, V.H., Sahlu, T., Goetsch, A.L. 2012. Effects of different fresh-cut forages and their hays on feed intake, digestibility, heat production, and ruminal methane emission by Boer x Spanish goats. Journal of Animal Science. 90(8):2754-2762.
Varel, V.H., Wells, J., Shelver, W.L., Rice, C., Armstrong, D.L., Parker, D.B. 2012. Effect of anaerobic digestion temperature on odour, coliforms and chlortetracycline in swine manure or monensin in cattle manure. Journal of Applied Microbiology. 112:705-715.
May, K.D., Wells, J., Maxwell, C.V., Oliver, W.T. 2012. Granulated lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology of 10-day-old pigs. Journal of Animal Science. 90(4):1118-1125.
Berry, E.D., Wells, J. 2012. Soil solarization reduces Escherichia coli O157:H7 and total Escherichia coli on cattle feedlot pen surfaces. Journal of Food Protection. 75(1):7-13.
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Bosilevac, J.M., Wheeler, T.L. 2011. Impact of reducing the level of wet distillers grains fed to cattle prior to harvest on prevalence and levels of Escherichia coli O157:H7 in feces and on hides. Journal of Food Protection. 74(10):1611-1617.
Wells, J., Varel, V.H. 2011. Chapter 9: Symbiosis of plants, animals, and microbes. In: Pond, W. G., Bazer, F. W., Rollin, B. E. (ed). Animal Welfare in Animal Agriculture: Husbandry, Stewardship and Sustainability. New York: CRC Press. p. 185-203.
Spiehs, M.J., Woodbury, B.L., Doran, B., Eigenberg, R.A., Kohl, K., Varel, V.H., Berry, E.D., Wells, J. 2010. Environmental conditions in beef deep-bedded mono-slope facilities. Iowa State University Animal Industry Report. Leaflet Number R2582.
May, K.D., Wells, J., Maxwell, C.V., Oliver, W.T. 2011. Granulated lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology of 10-day-old pigs [abstract]. Journal of Animal Science Supplement. 89(E-Supplement 2):71-72. Abstract # 83.
Casas, E., Garcia, M.D., Wells, J., Smith, T.P.L. 2011. Association of single nucleotide polymorphisms in the ANKRA2 and CD180 genes with bovine respiratory disease and presence of Mycobacterium avium subsp. paratuberculosis. Animal Genetics. 42:571-577.
Casas, E., Garcia, M.D., Wells, J., Smith, T.P. 2011. Association of single nucleotide polymorphisms in the ANKRA2 and CD180 genes with bovine respiratory disease and presence of Mycobacterium avium subsp. paratuberculosis [abstract]. Plant and Animal Genome XIX Conference. Poster No. P193.
Casas, E., Kuehn, L.A., Snelling, W.M., Wells, J. 2010. Genomics of Disease in Beef Cattle. Proceedings of the 9th World Congress on Genetics Applied to Livestock Production, Leipzig, Germany. August 1-6, 2010. CD-ROM Communication No. 0125.
Berry, E.D., Wells, J. 2010. Escherichia coli O157:H7: Recent Advances in Research on Occurrence, Transmission, and Control in Cattle and the Production Environment. In: Taylor, Steve L. Advances in Food and Nutrition Research. Volume 60. Burlington: Elsevier. p. 67-118.
Berry, E.D., Wells, J., Arthur, T.M., Woodbury, B.L., Nienaber, J.A., Brown Brandl, T.M., Eigenberg, R.A. 2010. Soil Versus Pond Ash Surfacing of Feedlot Pens: Occurrence of Escherichia coli O157:H7 in Cattle and Persistence in Manure. Journal of Food Protection. 73(7):1269-1277.
Varel, V.H., Wells, J., Berry, E.D., Miller, D.N. 2010. Odor Production and Escherichia coli Concentrations in Manure Slurries of Feedlot Steers Fed 0 or 40% Corn Wet Distillers Grains with Solubles. In: Proceedings of the 110th General Meeting of the American Society for Microbiology, May 23-27, 2010, San Diego, CA. 2010 CDROM.
Arthur, T.M., Bosilevac, J.M., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmarie, M. 2010. Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle. Journal of Food Protection. 73(2):366-371.
Berry, E.D., Millner, P.D., Wells, J., Kalchayanand, N. 2009. Evaluation of the Fate of Naturally Occurring Escherichia coli O157:H7 During Bovine Manure Composting Processes. [abstract] In: Proceedings 2009 International Annual Meetings of Agronomy Society of America, Crop Science Society of America, Soil Science Society of America, 1-5 Nov 2009, Pittsburgh, PA. CDROM.
Wells, J., Oliver, W.T., Yen, J. 2010. The Effects of Dietary Additives on Faecal Levels of Lactobacillus spp., Coliforms, and Escherichia coli, and Faecal Prevalence of Salmonella spp. and Campylobacter spp. in U.S. Production Nursery Swine. Journal of Applied Microbiology. 108:306-314.
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Guerini, M.N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles. Journal of Food Protection. 72(8):1624-1633.
Wells, J., Bosilevac, J.M., Kalchayanand, N., Arthur, T.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States. Journal of Food Protection 72(7):1457-1462.
Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles [abstract]. Microbial Ecology 57(3):583-584.
Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States [abstract]. Institute of Food Technologists (IFT), Division Food Microbiology, Session 026-15, p. 82.
Bosilevac, J.M., Wells, J., Arthur, T.M., Kalchayanand, N., Shackelford, S.D., Berry, E.D., Bono, J.L., Harhay, D.M., Wheeler, T.L., Koohmaraie, M. 2009. Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.
Brown Brandl, T.M., Berry, E.D., Wells, J., Arthur, T.M., Nienaber, J.A. 2009. Impacts of Individual Animal Response to Heat and Handling Stresses on Escherichia coli and E. coli O157:H7 Fecal Shedding by Feedlot Cattle. Foodborne Pathogens and Disease. 6(7):855-864.
Berry, E.D., Wells, J. 2008. A direct plating method for estimating populations of Escherichia coli 0157 in bovine manure and manure-based materials. Journal of Food Protection 71(11):2233-2238.
Brown Brandl, T.M., Berry, E.D., Wells, J., Arthur, T.M., Nienaber, J.A. 2008. The impact of stress level on fecal bacteria and pathogen shedding in feedlot cattle. American Society of Agricultural and Biological Engineers. Paper No. 084178. St. Joseph, MI:ASABE.
Puchala, R., Animut, G., Goetsch, A.L., Patra, A.K., Sahlu, T., Varel, V.H., Wells, J. 2008. Ruminal Methane Emissions by Goats Consuming Dry Hay of Condensed Tannin-Containing Lespedeza With or Without Polyethylene Glycol, Alfalfa, or Sorghum-Sudangrass [abstract]. In: Proceedings of 9th International Conference on Goats, Aug. 31 - Sept. 5, 2008, Queretaro, Mexico. Abstract 73, pp. 98-99.
Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Prevalence and characterization of Salmonella in bovine lymph nodes potentially destined for use in ground beef. Journal of Food Protection 71(8):1685-1688.
Varel, V.H., Wells, J., Berry, E.D., Spiehs, M.J., Miller, D.N., Ferrell, C.L., Shackelford, S.D., Koohmaraie, M. 2008. Odorant production and persistence of generic E. coli in manure slurries from cattle fed 0, 20, 40, and 60% wet distillers grains with solubles (WDGS)[abstract]. Journal of Animal Science 86(E-Suppl. 2)/Journal of Dairy Science 91(E-Suppl. 1):325.
Wells, J., Varel, V.H. 2008. Viability of zoonotic pathogens Escherichia coli and Salmonella in swine manure slurries with and without a urease inhibitor and thymol. Letters in Applied Microbiology 46:477-482.
Animut, G., Puchala, R., Goetsch, A.L., Patra, A.K., Sahlu, T., Varel, V.H., Wells, J. 2008. Methane emission by goats consuming diets with different levels of condensed tannins from lespedeza. Animal Feed Science and Technology 144:212-227.
Berry, E.D., Woodbury, B.L., Nienaber, J.A., Eigenberg, R.A., Thurston Enriquez, J.A., Wells, J. 2007. Incidence and persistence of zoonotic bacterial and protozoan pathogens in a beef cattle feedlot runoff control - vegetative treatment system. Journal of Environmental Quality 36:1873-1882.
Varel, V.H., Wells, J., Berry, E.D. 2007. Effect of N-(n-butyl) Phosphoric Triamide (NPBT) and a Linalool or Pine Oil Extract on Urea Concentration, Odorants, and Coliform Bacteria in Cattle Feedlot Manure. In CDRom Proceedings: American Society of Agri Engineers Special Meetings, Sept. 15-19, 2007, Broomfield, CO. CDRom 701P0907cd.
Varel, V.H., Wells, J., Miller, D.N. 2007. Combination of a urease inhibitor and a plant essential oil to control coliform bacteria, odour production, and ammonia loss from cattle waste. Journal of Applied Microbiology 102:472-477. doi:10.1111/j.1365-2672.2006.03120.x
Varel, V.H., Wells, J. 2007. Influence of thymol and a urease inhibitor on coliform bacteria, odor, urea, and methane from a swine production manure pit. Journal of Environmental Quality 36(3):773-779.
Berry, E.D., Wells, J.E., Archibeque, S.L., Ferrell, C.L., Freetly, H.C., Miller, D.N. 2006. Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. II. Pathogenic and other fecal bacteria. Journal of Animal Science. 84:2523-2532.
Miller, D.N., Berry, E.D., Wells, J.E., Ferrell, C.L., Archibeque, S.L., Freetly, H.C. 2006. Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. III. Odorous compound production. Journal of Animal Science. 84:2533-2545.
Animut, G., Puchala, R., Goetsch, A., Sahlu, T., Detweiler, G., Patra, A., Varel, V., Wells, J. 2006. Methane emission by goats consuming diets with different levels of condensed tannin-containing lespedeza and sorghum-sudangrass [abstract]. Journal of Animal Science 84(Suppl. 1):26.
Varel, V.H., Wells, J. 2006. Influence of thymol on coliform bacteria, VFA, and methane production from pull-plug swine manure pits [abstract]. Journal of Animal Science 84(Suppl. 1):292-293.
Wells, J., Berry, E.D., Varel, V.H. 2006. Effects of essential oils on viability of Escherichia coli O157:H7 in treated beef cattle manure slurries and on prevalence from treated feedlot surfaces [abstract]. Journal of Animal Science 84(Suppl 1):356-357.
Wells, J., Varel, V.H. 2005. Viability of Salmonella enterica Typhimurium and Escherichia coli O157:H7 in finishing swine manure slurries with and without a urease inhibitor and a plant essential oil [abstract]. Journal of Animal Science. 83(Supplement 1):301-302.
Wells, J., Yen, J. 2005. The effects of larch extract on performance and fecal flora in nursery pigs. Proceedings of the 2005 Conference on Gastrointestinal Function. p. 18.
Varel, V.H., Wells, J. 2005. Plant oils and urease inhibitors as solutions for environmental issues associated with confined animal feeding operations. Proceedings of the 2005 Conference on Gastrointestinal Function. p. 28.
Wells, J.E., Yen, J.T., Miller, D.N. 2005. Impact of dried skim milk in production diets on lactobacillus and pathogenic bacterial shedding in growing-finishing swine. Journal of Applied Microbiology 99:400-407.
Yen, J., Wells, J., Miller, D.N. 2004. Dried skim milk as a replacement for soybean meal in growing-finishing diets: Effects on growth performance, apparent total-tract nitrogen digestibility, urinary and fecal nitrogen excretion, and carcass traits in pigs. Journal of Animal Science. 82:3338-3345.
Varel, V.H., Wells, J., Miller, D.N. 2004. Combination of a urease inhibitor and a plant essential oil for control of fecal coliforms and emissions of odor and ammonia from cattle waste [abstract]. Journal of Animal Science 82(Suppl. 1):302.
Wells, J.E., Berry, E.D., Miller, D.N., Varel, V.H. 2004. Effect of plant phenolics on Escherichia coli O157:H7 survival in bovine feces. In: Proceedings of the 104th General Meeting of the American Society for Microbiology, May 23-27, 2004, New Orleans, Louisiana. 2004 CDROM.
WELLS, J., WILLIAMS, K.B., WHITEHEAD, T.R., HEUMAN, D.M., HYLEMON, P.B. 2003. DEVELOPMENT AND APPLICATION OF A POLYMERASE CHAIN REACTION ASSAY FOR THE DETECTION AND ENUMERATION OF BILE ACID 7 ALPHA-DEHYDROXYLATING BACTERIA IN HUMAN FECES. CLINICA CHIMICA ACTA. 331:127-134.