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United States Department of Agriculture

Agricultural Research Service

Douglas B Jordan
Bioenergy Research Unit
Research Molecular Biologist

Phone: (309) 681-6472
Fax: (309) 681-6427
Room 2131B
1815 N UNIVERSITY ST
PEORIA, IL, 61604

Projects
ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES
Appropriated (D)
  Accession Number: 417041

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation Reprint Icon - (Peer Reviewed Journal)
Singh, S.K., Heng, C., Braker, J.D., Chan, V.J., Lee, C.C., Jordan, D.B., Yuan, L., Wagschal, K.C. 2013. Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation. Journal of Industrial Microbiology and Biotechnology. 41(3):489-498. DOI: 10.1007/s10295-013-1377-0.
Rehabilitation of faulty kinetic determinations and misassigned glycoside hydrolase family of retaining mechanism ß-xylosidases - (Peer Reviewed Journal)
Jordan, D.B., Vermillion, K., Grigorescu, A.A., Braker, J.D. 2013. Rehabilitation of faulty kinetic determinations and misassigned glycoside hydrolase family of retaining mechanism ß-xylosidases . Archives of Biochemistry and Biophysics. 537(2):176-184.
Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity - (Peer Reviewed Journal)
Jordan, D.B., Lee, C.C., Wagschal, K., Braker, J.D. 2013. Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity. Archives of Biochemistry and Biophysics. 533:79-87.
Divalent metal activation of a GH43 ß-xylosidase - (Peer Reviewed Journal)
Lee, C.C., Braker, J.D., Grigorescu, A.A., Wagschal, K.C., Jordan, D.B. 2013. Divalent metal activation of a GH43 ß-xylosidase. Enzyme and Microbial Technology. 52(2):84-90.
Highly active ß-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates - (Peer Reviewed Journal)
Jordan, D.B., Wagschal, K.C., Grigorescu, A.A., Braker, J.D. 2013. Highly active ß-xylosidases of glycoside hydrolase family 43 operating on natural and artificial substrates. Applied Microbiology and Biotechnology. 97:4415-4428.
Plant cell walls to ethanol. - (Peer Reviewed Journal)
Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
Opposing influences by subsite -1 and subsite +1 residues on relative xylopyranosidase/arabinofuranosidase activities of bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D. 2011. Opposing influences by subsite -1 and subsite +1 residues on relative xylopyranosidase/arabinofuranosidase activities of bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase. Biochimica et Biophysica Acta. 1814:1648-1657.
Kinetic mechanism of an aldehyde reductase of Saccharomyces cerevisiae that relieves toxicity of furfural and 5-hydroxymethylfurfural - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D., Bowman, M.J., Vermillion, K., Moon, J., Liu, Z. 2011. Kinetic mechanism of an aldehyde reductase of Saccharomyces cerevisiae that relieves toxicity of furfural and 5-hydroxymethylfurfural. Biochimica et Biophysica Acta. 1814:1686-1694.
Catalytic properties of ß-D-xylosidase XylBH43 from Bacillus halodurans C-125 and mutant XylBH43-W147G - (Peer Reviewed Journal)
Wagschal, K.C., Jordan, D.B., Braker, J.D. 2011. Expression, characterization, and site-directed mutagenesis of ß-D-xylosidase XylBH43 from Bacillus halodurans C-125. Process Biochemistry. doi:10.1016/j.procbio.2011.07.009.
Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145 - (Peer Reviewed Journal)
Jordan, D.B., Wagschal, K.C., Zhanmin, F., Yuan, L., Braker, J.D., Heng, C. 2011. Engineering lower inhibitor affinities in beta-D-xylosidase of Selenomonas ruminantium by site-directed mutagenesis of Trp145. Journal of Industrial Microbiology and Biotechnology. 38:1821-1835.
Stereochemistry of Furfural Reduction by a Saccharomyces cerevisiae Aldehyde Reductase That Contributes to In Situ Furfural Detoxification - (Peer Reviewed Journal)
Bowman, M.J., Jordan, D.B., Vermillion, K., Braker, J.D., Moon, J., Liu, Z. 2010. Stereochemistry of Furfural Reduction by a Saccharomyces cerevisiae Aldehyde Reductase That Contributes to In Situ Furfural Detoxification. Applied and Environmental Microbiology. 76(15):4926-4932.
Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris - (Peer Reviewed Journal)
Mertens, J.A., Braker, J.D., Jordan, D.B. 2010. Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris. Applied Biochemistry and Biotechnology. 162(8):2197-2213.
and applications of microbial ß-D-xylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium - (Peer Reviewed Journal)
Jordan, D.B., Wagschal, K.C. 2010. Properties and applications of microbial beta-D-xylosidases. Applied Microbiology and Biotechnology. 86(6):1647-1658
Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145 - (Abstract Only)
Jordan, D.B., Wagschal, K.C., Fan, Z., Yuan, L., Braker, J.D., Heng, C. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145. Meeting Abstract. 84.
Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-Directed Mutagenesis, Alternate Substrates, and Active-site Inhibitor - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D. 2010. Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-directed Mutagenesis, Alternate Substrates, and Active-site Inhibitor. Applied Biochemistry and Biotechnology. 161(1-8):395-410.
Engineering Lower Inhibitor Affinities in Beta-D-Xylosidase - (Peer Reviewed Journal)
Fan, Z., Yuan, L., Jordan, D.B., Wagschal, K.C., Heng, C., Braker, J.D. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113.
Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-directed Mutagenesis, Alternate Substrates, and Inhibitor - (Abstract Only) - (31-Jul-09)
Beta-D-xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and inhibitors - (Abstract Only) - (06-May-09)
Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Abstract Only)
Bischoff, K.M., Jordan, D.B., De Rezende, S.T., Rich, J.O. 2009. Hemicellulolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Biotechnology for Fuels and Chemicals. Poster No. 5-57. p. 120.
Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Peer Reviewed Journal)
Bischoff, K.M., Wicklow, D.T., Jordan, D.B., De Rezende, S.T., Liu, S., Hughes, S.R., Rich, J.O. 2009. Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae. Current Microbiology. 58:499-503.
Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D. 2009. Beta-D-xylosidase from Selenomonas ruminantium: Thermodynamics of Enzyme-catalyzed and Noncatalyzed Reactions. Applied Biochemistry and Biotechnology. 155(1-3):330-346.
Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium - (Peer Reviewed Journal)
Jordan, D.B., Mertens, J.A., Braker, J.D. 2009. Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium. Biochimica et Biophysica Acta. 1794(1):144-158.
Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions - (Abstract Only) - (18-Jul-08)
Enzymes, Industrial - (Book / Chapter)
Saha, B.C., Bothast, R.J., Jordan, D.B. 2009. Enzymes, Industrial (Overview). In: Schaechter, M., editor. Encyclopedia of Microbiology. Oxford: Elsevier. p. 281-294.
Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions - (Abstract Only) - (07-May-08)
Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural substrates - (Abstract Only) - (07-May-08)
Biofuels Group NSF DUSEL Project - (Abstract Only)
Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
Structure of the two-subsite beta-D-xylosidase from Selenomonas ruminantium in complex with 1,3-bis[tris(hydroxymethyl)methylamino]propane - (Peer Reviewed Journal)
Brunzelle, J.S., Jordan, D.B., McCaslin, D.R., Olczak, A., Wawrzak, Z. 2008. Structure of the two-subsite beta-D-xylosidase from Selenomonas ruminantium in complex with 1,3-bis[tris(hydroxymethyl)methylamino] propane. Archives Of Biochemistry and Biophysics. 474(1):157-166.
Beta-D-Xylosidase from Selenomonas Ruminantium: Catalyzed Reactions with Natural and Artificial Substrates - (Peer Reviewed Journal)
Jordan, D.B. 2008. Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural and artificial substrates. Applied Biochemistry and Biotechnology. 146:137-149.
Structure-Function Relationships of a Catalytically-Efficient Beta-D-Xylosidase - (Abstract Only) - (03-Aug-07)
Variation in Relative Substrate Specificty of Bifunctional Beta-D-Xylosidase/alpha-L-Arabinofuranosidase by Single-Site Mutations: Roles of Substrate Distortion and Recognition - (Peer Reviewed Journal)
Jordan, D.B., Li, X. 2007. Variation in relative substrate specificity of bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase by single-site mutations: roles of substrate distortion and recognition. Biochimica et Biophysica Acta. 1774(9):1192-1198.
Inhibition of the Two-Subsite Beta-D-Xylosidase from Selenomonas Ruminantium by Sugars: Competitive, Noncompetitive, Double Binding, and Slow Binding Modes - (Peer Reviewed Journal)
Jordan, D.B., Braker, J.D. 2007. Inhibition of the two-subsite beta-d-xylosidase from Selenomonas ruminantium by sugars: competitive, noncompetitive, double binding, and slow binding modes. Archives of Biochemistry and Biophysics. 465(1):231-246.
Beta-D-Xylosidase from Selenomonas Ruminantium: Thermodynamics of Enzyme-Catalyzed and Noncatalyzed Reactions - (Abstract Only)
Jordan, D.B. 2007. Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions [abstract]. Biotechnology for Fuels and Chemicals. p. 105.
Beta-D-Xylosidase from Selenomonas Ruminantium: Catalyzed Reactions with Natural and Artificial Substrates - (Abstract Only)
Jordan, D.B. 2007. Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural and artificial substrates [abstract]. Biotechnology for Fuels and Chemicals. p. 106.
Structure-Function Relationships of a Catalytically Efficient Beta-D-Xylosidase - (Abstract Only)
Jordan, D.B. 2007. Structure-function relationships of a catalytically efficient beta-D-xylosidase [abstract]. American Chemical Society. Paper No. 175.
Production of Highly Active Lignocellulose-Degrading Enzymes of Anaerobic Fungi by Industrially Relevant Fungi - (Abstract Only)
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract]. Physiology to Genomics to Fuels.
Expression of An at-Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Sp. Strain Pc-2 in and Secretion of the Heterologous Enzyme by Hypocrea Jecorina - (Peer Reviewed Journal)
Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.
Fungicidal Carboxamides - (Other)
Basarab, G.S., Jordan, D.B., Lessen, T.A., Hansen, S.L. 2006. Fungicidal carboxamides. U.S. Patent 7,135,585.
Beta-D-Xylosidase from Selenomonas Ruminantium of Glycoside Hydrolase Family 43 - (Peer Reviewed Journal)
Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2007. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43. Applied Biochemistry and Biotechnology. 136-140:93-104.
Hemicellulases for Mediating Biomass Saccharification - (Abstract Only) - (13-Oct-06)
Hemicellulases for Mediating Biomass Saccharification - (Proceedings)
Jordan, D.B., Dien, B.S., Li, X., Cotta, M.A. 2006. Hemicellulases for mediating biomass saccharification. In: Proceedings of Renewable Energy 2006, October 9-13, 2006, Makuhari Messe, Chiba, Japan. p. 1036-1041.
Beta-D-Xylosidase from Selenomonas Ruminantium of Glycoside Hydrolase Family 43 - (Abstract Only)
Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2006. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43 [abstract]. Gordon Research Conference. p. 3.
Beta-D-Xylosidase from Selenomonas Ruminantium of Glycoside Hydrolase Family 43 - (Abstract Only)
Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2006. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43 [abstract]. Biotechnology for Fuels and Chemicals. p.93
Codon Optimization of An A+t Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Pc-2 for Expression by Trichoderma Reesei - (Abstract Only)
Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.
Enzymatic Saccharification of Hot-Water Pretreated Corn Fiber for Production of Monosaccharides - (Peer Reviewed Journal)
Dien, B.S., Li, X., Iten, L.B., Jordan, D.B., Nichols, N.N., O Bryan, P.J., Cotta, M.A. 2006. Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides. Enzyme and Microbial Technology. 39:1137-1144.
Heterologous Production by Trichoderma Reesei of a Xylanase of the Anaerobic Fungus Orpinomyces Pc-2 - (Abstract Only) - (09-Nov-05)
Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract Only)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.
Active-Site Models of Riboflavin Synthase - (Proceedings)
Jordan, D.B., Calabrese, J.C., Liao, D., Wawrzak, Z., Zheng, Y. 2005. Active-site models of riboflavin synthase. In: Nishino, T., Miura, R., Tanokura, M., Fukui, K., editors. Flavins and Flavoproteins 2005. Tokyo: ARchiTect, Inc. p. 737-742.
Functional Proteomic Workcell for High Volume Plasmid Preparations for Repeated in Vitro Protein Expression and High Throughput Screening to Identify Mutant Enyzmes for Use at Low Ph - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.
Enzymatic Saccharification of Pretreated Corn Fiber for Production of Sugars - (Abstract Only)
Dien, B.S., Li, X., Jordan, D.B., Nichols, N.N., Iten, L.B., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. International Starch Technology. p. 90.
Active-Site Models of Riboflavin Synthase - (Abstract Only)
Jordan, D.B., Calabrese, J.C. 2005. Active-site models of riboflavin synthase [abstract]. International Symposium on Flavins and Flavoproteins. p. 104.
Substrate Specificities of Two Glycoside Hydrolase Family 11 Xylanases - (Abstract Only)
Li, X., Jordan, D.B., Cotta, M.A. 2005. Substrate specificities of two glycoside hydrolase family 11 xylanases [abstract]. American Chemical Society. Paper No. 066.
Advances on Enzymatic Saccharification of Lignocellulosic Biomass - (Abstract Only)
Li, X., Jordan, D.B., Dien, B.S., Cotta, M.A., Kane, P.M. 2004. Advances on enzymatic saccharification of lignocellulosic biomass [abstract]. Great Lakes Regional American Chemical Society. Paper No. 04-035.
Crystal Structure of Phenylalanine Ammonia Lyase: Multiple Helix Dipoles Implicated in Catalysis - (Peer Reviewed Journal)
Calabrese, J.C., Jordan, D.B., Boodhoo, A., Sariaslani, S., Vannelli, T. 2004. Crystal structure of phenylalanine ammonia lyase: multiple helix dipoles implicated in catalysis. Journal of Biochemistry. 43:11403-11416.
Last Modified: 4/17/2014
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