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United States Department of Agriculture

Agricultural Research Service

Related Topics

Clifton Fagerquist (Keith)
Produce Safety and Microbiology Research
Research Chemist

Phone: (510) 559-5691
Fax: (510) 559-6162
Room 3100
USDA, ARS, PWA, WRRC-PSM
800 BUCHANAN STREET
ALBANY, CA, 94710

Projects
Molecular Biology of Human Pathogens Associated with Food
Appropriated (D)
  Accession Number: 421039

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Two Shiga toxin 2 subtypes in a single Shiga toxin-producing Escherichia coli analyzed by RT-qPCR, MALDI-TOF-TOF-MS and top-down proteomic analysis - (Proceedings) - (06-Jun-14)
Top-down proteomic identification of Shiga toxin 2 subtypes from Shiga toxin-producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight mass spectrometry - (Peer Reviewed Journal)
Fagerquist, C.K., Zaragoza, W.J., Sultan, O., Woo, N., Quinones, B., Cooley, M.B., Mandrell, R.E. 2014. Top-down proteomic identification of Shiga toxin 2 subtypes from Shiga toxin-producing Escherichia coli by Matrix-Assisted Laser Desorption Ionization-Tandem Time of Flight mass spectrometry. Applied and Environmental Microbiology. 8(9):2928-2940.
Peptides indentified in soybean protein increase plasma cholesterol in mice on hypercholesterolemic diets Reprint Icon - (Peer Reviewed Journal)
Zhang, H., Bartley, G.E., Zhang, H., Wang, J., Fagerquist, C.K., Zhong, F., Yokoyama, W.H. 2013. Peptides indentified in soybean protein increase plasma cholesterol in mice on hypercholesterolemic diets. Journal of Agricultural and Food Chemistry. DOI: 10.1021/jf4022288.
The need for agriculture phenotyping: “Moving from genotype to phenotype” - (Peer Reviewed Journal)
Boggess, M.V., Lippolis, J.D., Hurkman II, W.J., Fagerquist, C.K., Briggs, S.P., Gomes, A.V., Righetti, P., Bala, K. 2013. The need for agriculture phenotyping: “Moving from genotype to phenotype”. Journal of Proteomics. doi: http://dx.doi.org/10.1016/j.jptot.2013.3.021.
Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflamatory bowel disease Reprint Icon - (Peer Reviewed Journal)
Elliott, T.R., Hudspith, B.N., Wu, G., Cooley, M.B., Parkes, G., Quinones, B., Randall, L., Mandrell, R.E., Fagerquist, C.K., Brostoff, J., Rayment, N.B., Boussioutas, A., Petrovska, L., Sanderson, J.D. 2013. Quantification and characterization of mucosa-associated and intracellular Escherichia coli in inflamatory bowel disease. Inflammatory Bowel Diseases. 19:11 2326-2338.
Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay - (Review Article)
Fagerquist, C.K. 2013. Top-down proteomic identification of bacterial protein biomarkers and toxins using MALDI-TOF-TOF-MS/MS and post-source decay. Reviews in Analytical Chemistry. 32(2):127-133. doi:10.1515/revac-2012-0035.
Possible evidence of amide bond formation between sinapinic acid and lysine-containing bacterial proteins by matrix-assisted laser desorption/ionization (MALDI) at 355 nm - (Peer Reviewed Journal)
Fagerquist, C.K., Sultan, O., Carter, M.Q. 2012. Possible evidence of amide bond formation between sinapinic acid and lysine-containing bacterial proteins by matrix-assisted laser desorption/ionization (MALDI) at 355 nm. Journal of American Society for Mass Spectrometry. 23(12):2102-2114.
RcsB contributes to the distinct stress fitness between Escherichia coli O157:H7 curli variants of 1993 hamburger-associated outbreak strains - (Peer Reviewed Journal)
Carter, M.Q., Parker, C., Louie, J.W., Huynh, S., Fagerquist, C.K., Mandrell, R.E. 2012. RcsB contributes to the distinct stress fitness between Escherichia coli O157:H7 curli variants of 1993 hamburger-associated outbreak strains. Applied and Environmental Microbiology. doi:10.0028/AEM.02157-12.
High sensitive and throughput screening of Aflatoxin using MALDI-TOF-TOF-PSD-MS/MS - (Abstract Only)
Hua, S.T., Woo, N., Sultan, O., Fagerquist, C.K. 2012. High sensitive and throughput screening of Aflatoxin using MALDI-TOF-TOF-PSD-MS/MS. Meeting Abstract. [abstract].
A new calibrant for MALDI-TOF-TOF-PSD-MS/MS of non-digested proteins for top-down proteomic analysis Reprint Icon - (Peer Reviewed Journal)
Fagerquist, C.K., Sultan, O. 2012. A new calibrant for MALDI-TOF-TOF-PSD-MS/MS of non-digested proteins for top-down proteomic analysis. Rapid Communications in Mass Spectrometry. 1241:1248.
Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7 Reprint Icon - (Peer Reviewed Journal)
Carter, M.Q., Louie, J.W., Fagerquist, C.K., Sultan, O., Miller, W.G., Mandrell, R.E. 2012. Evolutionary silence of the acid chaperone protein HdeB in enterohemorrhagic Escherichia coli O157:H7. Applied and Environmental Microbiology. 78:1004-1014.
Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS - (Book / Chapter)
Fagerquist, C.K., Sultan, O. 2012. Top-down proteomic identification of protein biomarkers of food-borne pathogens using MALDI-TOF-TOF-MS/MS. Lee, M.S. Editor. Mass Spectrometry Handbook. New Jersey:John Wiley & Sons. p. 559-573.
Molecular Insights into the Phenotypic Divergence in Natural Curli Variants of Escherichia coli O157:H7 - (Abstract Only) - (28-Feb-11)
Induction and identification of disulfide-intact and disulfide-reduced beta-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics - (Peer Reviewed Journal)
Fagerquist, C.K., Sultan, O. 2011. Induction and identification of disulfide-intact and disulfide-reduced beta-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS and top-down proteomics. Analyst.136:1739-1746. DOI: 10.1039/C0AN00909A.
Rapid Identification of Food-borne Pathogens by Top-Down Proteomics Using MALDI-TOF/TOF Mass Spectrometry Reprint Icon - (Book / Chapter)
Fagerquist, C.K. 2011. Rapid Identification of Food-borne Pathogens by Top-Down Proteomics Using MALDI-TOF/TOF Mass Spectrometry. In: Fenselau, C; Demirev, P.,editors. Rapid Characterization of Microorganisms by Mass Spectrometry. Washington D.C., American Chemistry Society Publications. American Chemical Society Symposium Series. 1065:99-120
Top-down proteomic identification of furin-cleaved alpha-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS - (Peer Reviewed Journal)
Fagerquist, C.K., Sultan, O. 2010. Top-down proteomic identification of furin-cleaved alpha-subunit of Shiga toxin 2 from Escherichia coli O157:H7 using MALDI-TOF-TOF-MS/MS. Journal of Biomedicine and Biotechnology. DOI:10.1155/2010/123460.
Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B.R., Miller, W.G., Williams, K.E., Yee, E., Bates, A.H., Boyle, S., Harden, L.A., Cooley, M.B., Mandrell, R.E. 2010. Rapid Identification of Protein Biomarkers of E. coli O157:H7 by MALDI-TOF-TOF Mass Spectrometry and Top-Down Proteomics. Analytical Chemistry. 82:2717-2725.
Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B., Williams, K., Bates, A.H., Harden, L.A. 2010. Covalent attachment and dissociative loss of sinapinic acid to/from cysteine-containing proteins from bacterial cell lysates analyzed by MALDI-TOF-TOF mass spectrometry. Journal of American Society for Mass Spectrometry. 21,819-832.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry - (Proceedings)
Fagerquist, C.K., Garbus, B.R., Williams, K.E., Bates, A.H., Boyle, S., Harden, L.A., Miller, W.G., Mandrell, R.E. 2009. Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry.IN: Proceedings of the 57th American Society of Mass Spectrometry Conference, May 31-June 4, 2009, Philadelphia, PA. DVD:A091307.8125ver.1.pdf
Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification - (Peer Reviewed Journal)
Fagerquist, C.K., Garbus, B.R., Williams, K., Bates, A.H., Boyle, S., Harden, L.A. 2009. Web-based software for rapid "top-down" proteomic identification of protein biomarkers with implications for bacterial identification. Applied and Environmental Microbiology. 75(13):4341-4353.
Rapid Identification of E. coli O157:H7 by "Top-Down" Proteomics Using MALDI-TOF/TOF Mass Spectrometry - (Abstract Only) - (10-Mar-09)
Proteomics of foodborne bacterial pathogens Reprint Icon - (Book / Chapter)
Fagerquist, C.K. 2011. Proteomics of foodborne bacterial pathogens. In: Wiedman, M.; Zhang, W., editors of: Genomics of foodborne bacterial pathogens. New York, NY: Springer. p. 343-402.
High Resolution Genotyping of Campylobacter Using PCR and High-Throughput Mass Spectrometry - (Peer Reviewed Journal)
Hannis, J., Manalili, S., Hall, T., Ranken, R., White, N., Sampath, R., Blyn, L., Ecker, D., Mandrell, R.E., Fagerquist, C.K., Bates, A.H., Miller, W.G., Hofstadler, S. 2008. High Resolution Genotyping of Campylobacter Using PCR and High-Throughput Mass Spectrometry. Journal of Clinical Microbiology.46(4) 1220-1225.
Composite sequence proteomic analysis of protein biomarkers of Campylobacter coli, C. lari and C. concisus for bacterial identification - (Peer Reviewed Journal)
Fagerquist, C.K., Yee, E., Miller, W.G. 2007. Composite sequence proteomic analysis of protein biomarkers of Campylobacter coli, C. lari and C. consisus for bacterial identification. The Analyst. 132(10):1010-1023.
Amino Acid Sequence Determination of Protein Biomarkers of Campylobacter upsaliensis and C. helveticus by 'Composite' Sequence Proteomic Analysis - (Peer Reviewed Journal)
Fagerquist, C.K. 2007. Amino Acid Sequence Determination of Protein Biomarkers of Campylobacter upsaliensis and C. helveticus by "Composite" Sequence Proteomic Analysis. Journal of Proteome Research. 6(7):2539-2549.
Identification of Foodborne Bacteria by High Energy Collision-Induced Dissociation of Their Protein Biomarkers by MALDI Tandem-Time-of-Flight Mass Spectrometry - (Proceedings)
Fagerquist, C.K., Williams, K.E., Bates, A. H. Identification of Foodborne Bacteria by High Energy Collision-Induced Dissociation of their Protein Biomarkers by MALDI Tandem-Time-of-Flight Mass Spectrometry. In: Proceedings of the 55th American Society of Mass Spectrometry Conference, June 3-7, 2007, Indianapolis, IN. [DVD MPT-327 from ASMS.]
Identification of the Molecular Species of Triacylglycerols by Hplc and An Acylacyl-Diacylglycerol (Tetraacylglycerol) by Using Resolution Electrospray Ionization Tadem Mass Spectrometry in Castor Oil - (Proceedings)
Lin, J.T., Arcinas, A.J., Fagerquist, C.K. 2006. Identification of the molecular species of triacylglycerols by hplc and an acylacyl-diacylglycerol (tetraacylglycerol) by using resolution electrospray ionization tadem mass spectrometry in castor oil. International Symposium on Biocatalysis and Biotechnology: Functional Food and Industrial Products.
Sub-Speciating Campylobacter Jejuni by Proteomic Analysis of Its Protein Biomarkers and Their Post-Translational Modifications - (Peer Reviewed Journal)
Fagerquist, C.K., Bates, A.H., Heath, S., King, B.C., Garbus, B.R., Harden, L.A., Miller, W.G. Sub-speciating campylobacter jejuni by proteomic analysis of its protein biomarkers and their post-translational modifications. Journal of Proteome Research. 5:10:2527-2538
Identification of (12-Ricinoleoyl-Ricinoleoyl)-Diricinoleoyl-Gylcerol, An Acylglycerol Containing Four Acyl Chains, in Castor by Lc-Esi-Ms - (Peer Reviewed Journal)
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-gylcerol, an acylglycerol containing four acyl chains, in castor by lc-esi-ms. Journal of Agriculture and Food Chemistry. 54(10):3498-3504.
Identification of (12-Ricinoleoyl-Ricinoleoyl)-Diricinoleoyl-Glycerol in Castor Oil by Esi-Ms/ms and Its Biosynthesis - (Abstract Only)
Lin, J.T., Arcinas, A.J., Harden, L.A., Fagerquist, C.K. 2006. Identification of (12-ricinoleoyl-ricinoleoyl)-diricinoleoyl-glycerol in castor oil by esi-ms/ms and its biosynthesis. American Oil Chemists' Society Meeting.
Implication of C-Terminal Deletion on the Structure and Stability of Bovine B-Casein - (Peer Reviewed Journal)
Qi, P.X., Wickham, E.D., Piotrowski, E.G., Fagerquist, C.K., Farrell Jr, H.M. 2005. Implication of c-terminal deletion on the structure and stability of bovine b-casein. Protein Journal. 24(7-8):431-444.
Genomic and Proteomic Identification of a Dna-Binding Protein Used in the "fingerprinting" of Campylobacter Species and Strains by Maldi-Tof-Ms Protein Biomarker Analysis - (Peer Reviewed Journal)
Fagerquist, C.K., Miller, W.G., Harden, L.A., Bates, A.H., Vensel, W.H., Wang, G., Mandrell, R.E. 2005. Genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. Analytical Chemistry. web release 6/25/05, 77:4897-4907
Speciation of Campylobacter Coli, C. Jejuni, C. Helveticus, C. Lari, C. Sputorum, and C. Upsaliensis by Matrix-Assisted Laser Desorption Ionization-Time of Flight (Maldi-Tof) Mass Spectrometry - (Peer Reviewed Journal)
Mandrell, R.E., Harden, L.A., Bates, A.H., Miller, W.G., Haddon, W.F., Fagerquist, C.K. 2005. Speciation of campylobacter coli, c. jejuni, c. helveticus, c. lari, c. sputorum, and c. upsaliensis by matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry. Applied and Environmental Microbiology. 71:10 p. 6292-6307
Confirmatory and Quantitative Analysis of B-Lactam Antibiotics in Bovine Kidney Tissue by Dispersive Solid-Phase Extraction and Liquid Chromatography-Tandem Mass Spectrometry - (Peer Reviewed Journal)
Fagerquist, C.K., Lightfield, A.R., Lehotay, S.J. 2005. Confirmatory and quantitative analysis of b-lactam antibiotics in bovine kidney tissue by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry. Analytical Chemistry 5(77).1473-1482.
Fingerprinting of Campylobacter Species and Strains by Mass Spectrometric Identification of Protein Biomarkers - (Proceedings)
Fagerquist, C.K., Harden, L.A., Miller, W.G., Fouts, D., Nelson, K., Mandrell, R.E. 2004. Fingerprinting of campylobacter species and strains by mass spectrometric identification of protein biomarkers. In: Proceedings of the 52nd American Society for Mass Spectrometry Conference and Allied Topics. May 23-27, 2004 Nashville, TN. CDROM
Collision-Activated Cleavage of a Peptide/antibiotic Disulfide Linkage: Evidence of Intramolecular Disulfide Bond Rearrangement Upon Collisional Activation - (Peer Reviewed Journal)
FAGERQUIST, C.K. COLLISION-ACTIVATED CLEAVAGE OF A PEPTIDE/ANTIBIOTIC DISULFIDE LINKAGE: EVIDENCE OF INTRAMOLECULAR DISULFIDE BOND REARRANGEMENT UPON COLLISIONAL ACTIVATION. RAPID COMMUNICATIONS IN MASS SPECTROMETRY.
Collison-Activated Cleavage of a Peptide/antiobiotic Linkage: Evidence for Gas-Phase Intramolecular Disulfide Exchange - (Abstract Only)
FAGERQUIST, C.K. COLLISON-ACTIVATED CLEAVAGE OF A PEPTIDE/ANTIOBIOTIC LINKAGE: EVIDENCE FOR GAS-PHASE INTRAMOLECULAR DISULFIDE EXCHANGE. AMERICAN SOCIETY FOR MASS SPECTROMETRY. 2002.
Analysis for B-Lactam Antibiotics in Kidney Tissue by Liquid Chromatography with Electrospray Ionization and Selective Reaction Monitoring/tandem Ion Trap Mass Spectrometry - (Peer Reviewed Journal)
FAGERQUIST, C.K., LIGHTFIELD, A.R. ANALYSIS FOR B-LACTAM ANTIBIOTICS IN KIDNEY TISSUE BY LIQUID CHROMATOGRAPHY WITH ELECTROSPRAY IONIZATION AND SELECTIVE REACTION MONITORING/TANDEM ION TRAP MASS SPECTROMETRY. RAPID COMMUNICATIONS IN MASS SPECTROMETRY. 2003.
A Bioactive Antiobiotic Linked to Peptides Is Released by Electron Capture Dissociation Fourier Transform Ion Cyclotron Resonance Mass Spectrometry - (Peer Reviewed Journal)
FAGERQUIST, C.K., HUDGINS, R.R., EMMETT, M.R., HAKANSSON, K., MARSHALL, A.G. A BIOACTIVE ANTIOBIOTIC LINKED TO PEPTIDES IS RELEASED BY ELECTRON CAPTURE DISSOCIATION FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY. JOURNAL OF AMERICAN SOCIETY FOR MASS SPECTROMETRY. 2003.
Selective Cleavage of a Small Antibiotic Drug Molecule from a Peptide and Protein in the Gas Phase Using Electron Capture Dissociation and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry - (Abstract Only)
FAGERQUIST, C.K., HUDGINS, R.R., EMMETT, M.R., HAKANSSON, K., MARSHALL, A.G. SELECTIVE CLEAVAGE OF A SMALL ANTIBIOTIC DRUG MOLECULE FROM A PEPTIDE AND PROTEIN IN THE GAS PHASE USING ELECTRON CAPTURE DISSOCIATION AND FOURIER TRANSFORM ION CYCLOTRON RESONANCE MASS SPECTROMETRY. MEETING ABSTRACT. 2002.
Approaches and Recommendations to Validate Ms Confirmation Criteria for Veterinary Drug Residues in Food - (Abstract Only)
LEHOTAY, S.J., SCHNEIDER, M.J., FAGERQUIST, C.K., LIGHTFIELD, A.R. APPROACHES AND RECOMMENDATIONS TO VALIDATE MS CONFIRMATION CRITERIA FOR VETERINARY DRUG RESIDUES IN FOOD. 116TH AOAC INTERNATIONAL ANNUAL MEETING. ABSTRACT. 2002. P. 80.
Last Modified: 7/25/2014
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