Degrees:

B.S.ed. Illinois State University

B.S.  Illinois State University

M.S. Miami University, Oxford Ohio

Ph.D. University of Nebraska, Lincoln

 

Research Interests:

Microbial ecology of pathogens in manure, and manure-impacted environments.

 

Major Project Activities:

My current projects focus on the survival of enteric pathogens in manure-amended soil, and feedlot runoff water, and the ecology of antibiotic resistant bacteria in manure-impacted soil and water.  Previous projects have focused on the pre-harvest control of E. coli O157:H7 in beef cattle feedlots, including the ecology and microbial community composition of the E. coli habitats in the cattle intestine and the beef feedlot pen surface material.  Another project involved examining the role of pest flies in the transmission of E. coli O157:H7, O111:H8 and O26:H11, including the development and validation of serotype-specific culture methods, and molecular serotyping methods.  I have also been involved in numerous human E. coli O157:H7 outbreak investigations associated with agricultural fairs.  

  

Publications: 

L.M. Durso, D.N. Miller, and B.J. Wienhold.  Linking antibiotic resistance genes and antibiotic resistant bacteria in agricultural and non-agricultural metagenomes.  PLoS.  Accepted for publication 9/24/12.

 

C. Thayer, J.E. Gilley, L.M. Durso, D.B. Marx. 2012. Wheat strip effects on nutrient loads following manure application. Trans. ASABE. 55:439-449.

 

Bengtsson, J, M. Hartmann, M. Unterseher, P. Vaishampayan, K. Abarenkov, L.M. Durso, K.M. Eriksson, R.H. Nilsson.  2012. Megraft: A software package to graft ribosomal small subunit (16S/18S) fragments onto full-length sequences for accurate species richness and sequencing depth analysis in pyrosequencing-length metagenomes.  Research in Microbioloy. 163:407-412.

 

Durso, L.M., J.E. Wells, G.P. Harhay, W.C. Rice, L.A. Kuehn, J.L. Bono, S.D. Shackelford, T.L. Wheeler, and T.P.L. Smith.  2012. Comparison of bacterial communities in feces of beef cattle fed diets containing corn and wet distillers grain with solubles. Letters in Applied Micro 55:109-114.

 

C. Thayer, J.E. Gilley, L.M. Durso, and D.B. Marx. 2012. Runoff nutrient loads as affected by residue cover, manure application rate, and flow rate. Trans. ASABE. 55(1):249-258. 

 

Comstock, N., M. Towle, A. Warner, S. Reynolds, L. Durso, C. Campbell, M. Kiefer.  2012. Epidemiologic and occupational investigation of an Escherichia coli O111 Outbreak associated with a correctional facility dairy – Colorado, 2010.  Morb. Mortal. Wkly. Rpt.  61(9):149-152. 

 

 

Durso, L.M., J.E. Gilley, D.B. Marx, and B. Woodbury.  2011.  Effects of animal diet, manure application rate, and tillage on transport of microorganisms from manure-amended fields.  Appl Environ Microbiol.  77:6715-6717.

 

Gilley, J.E., L.M. Durso, R.A. Eigenberg, D.B. Marx, and B.L. Woodbury. 2011.   Narrow grass hedge control of nutrient transport following variable manure application.  Trans. of the ASABE.  54:847-855.

 

Durso, L.M., G.P. Harhay, J.L. Bono, and T.P.L. Smith.  2011.  Virulence-associated and antibiotic resistance genes of microbial populations in cattle feces analyzed using a metagenomic approach.  J. Microbiol. Methods –84:278-282.

 

Durso, L.M., G.P. Harhay, T.P.L. Smith, J.L. Bono, T.Z. DeSantis, and M.L. Clawson.  Bacterial community analysis of beef feedlots reveals that pen surface is distinct from feces. Foodborne Pathogens and Disease 8:647-649.

 

Gilley, J.E., L.M. Durso, R.A. Eigenberg, D.B. Marx, and B.L. Woodbury. 2010. Nutrient transport in runoff as affected by distillers grain diet and manure application rate. 2010.  Trans. of the ASABE, 53:1895-1902. http://hdl.handle.net/10113/47312

 

Durso, L. M., H. Maguire, A. Cronquist, and J. L. Bono.  Outbreak investigation: STEC O157.   Med Lab Obs 42:42-43, 2010.  http://hdl.handle.net/10113/44290

 

Durso, L. M., G. P. Harhay, T. P. L. Smith, J. L. Bono, T. Z. DeSantis, J. E. Keen, W. W. Laegreid, and M. L. Clawson.  Animal-to-Animal variation in fecal microbial diversity among beef cattle.   2010.  Appl. Environ. Microbiol. 76:4858-4862. http://aem.asm.org/cgi/reprint/76/14/4858

 

Keen, J. E., W. W. Laegreid, C. G. Chitko-McKown, L. M. Durso and J. L. Bono.  Gastrointestinal tract distribution at necropsy of Shiga-toxigenic Escherichia coli O157 in naturally infected cattle.  2010.  Appl. Environ. Microbiol. 76:5278-5281. http://hdl.handle.net/10113/44603

 

Clawson, M. L., J. E. Keen, T. P. L. Smith, L. M. Durso, T. G. McDaneld, R. E. Mandrell, M. A. Davis, and J. L. Bono.  2009.  Phylogenetic classification of Escherichia coli O157:H7 isolates of human and bovine origin using a novel set of nucleotide polymorphisms.  Genome Biol.  I0:R56.  Available:  (http://genomebiology.com/2009/10/5/R56)  http://hdl.handle.net/10113/40118

 

Goode, B., O’Reiley, C., Dunn, J., Fullerton, K., Smith, S., Ghneim, G., Keen, J., Durso, L., Davies, M., and Montgomery, S.  Outbreak of Escherichia coli O157.  Arch. Pediatr. Adolesc. Med. 163:42-48.  2009.

http://hdl.handle.net/10113/28293

 

Durso L. M. and Keen, J. E. Shiga-toxigenic Escherichia coli O157 and non-Shiga-toxigenic E. coli O157 respond differently to culture and isolation from naturally contaminated bovine faeces. J. Appl. Microbiol. 103(6):2457-2464. 2007. http://hdl.handle.net/10113/28096

 

Durso L. M., Bono, J. L. and Keen, J. E. Molecular serotyping of Escherichia coli O111:H8. J. Microbiol. Meth. 69(2):381-383. 2007. http://hdl.handle.net/10113/28067

 

Keen, J. E., Durso, L. M. and Meehan, T. P. Isolation of Salmonella enterica and Shiga-toxigenic Escherichia coli O157 from feces of animals in public contact areas of United States zoological parks. Appl. Environ. Microbiol. 73(1):362-365. 2007. http://hdl.handle.net/10113/28335

 

Bono, J. L., Keen, J. E., Clawson, M. L., Durso, L. M., Heaton, M. P. and Laegreid, W. W. Association of Escherichia coli O157:H7 tir polymorphisms with human infection. BMC Infect. Dis. 7:98. 2007. http://hdl.handle.net/10113/28101

 

Kimura, A. C., Mead, P., Walsh, B., Alfano, E., Kolavic-Grey, S., Durso, L., Humphrey, C., Monroe, S. S., Visvesvera, G., Puhr, N., Shieh, W.-J., Eberhard, M., Hoekstra, R. M. and Mintz, E. A large outbreak of brainerd diarrhea associated with a restaurant in the Red River Valley, Texas. Clin. Infect. Dis. 43:55-61. 2006.

 

Keen, J. E., Wittum, T. E., Dunn, J. R., Bono, J. L. and Durso, L. M. Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States. Emerg. Infect. Dis. 12(5):780-786. 2006. http://hdl.handle.net/10113/28066

 

Heaton, M. P., Keen, J. E., Clawson, M. L., Harhay, G. P., Bauer, N., Schultz, C., Green, B. T., Durso, L., Chitko-McKown, C. G. and Laegreid, W. W. Use of bovine single nucleotide polymorphism markers to verify sample tracking in beef processing. J. Am. Vet. Med. Assoc. 226(8):1311-1314. 2005. http://hdl.handle.net/10113/27953

 

Durso, L. M., Reynolds, K., Bauer, N. Jr. and Keen, J. E. Shiga-toxigenic Escherchia coli O157 infections among livestock exhibitors and visitors at a Texas county fair. Vector-Borne and Zoonotic Dis. 5(2):193-201. 2005. http://hdl.handle.net/10113/27954

 

Durso, L. M., Bono, J. L. and Keen, J. E. Molecular serotyping of Escherichia coli O26:H11. Appl. Environ. Microbiol. 71(8):4941-4944. 2005. http://hdl.handle.net/10113/19960

 

Centers for Disease Control and Prevention. Submitted by: Davies, M., Engel, J., Griffin, D., Ginzl, D., Hopkins, R., Blackmore, C., Lawaczec, E., Nathan, L., Levy, C., Briggs, G., Kioski, C., Kreis, S., Keen, J., Durso, L., Schulte, J., Fullerton, K., Long, C., Smith, S., Barton, C., Gleit, C., Joyner, M., Montgomery, S., Braden, C., Goode, B., Chertow, D., O’Reilly, C., Gupta, S. and Dunn, J. Outbreaks of Escherichia coli O157:H7 associated with petting zoos – North Carolina, Florida, and Arizona, 2004 and 2005. Morb. Mortal. Wkly. Rpt. 54(50):1277-1280. 2005.

 

Durso L. M., Smith, D. and Hutkins, R. W. Measurements of fitness and competition in commensal Escherichia coli and E. coli O157:H7 strains. Appl. Environ. Microbiol. 70(11):6466-6472. 2004.