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United States Department of Agriculture

Agricultural Research Service

Related Topics

Stephen Spatz
Endemic Poultry Viral Diseases Research Unit
Microbiologist

Phone: (706) 546-3463
Fax: (706) 546-3161
934 COLLEGE STATION ROAD
SPRL
ATHENS, GA, 30605

Projects
GENOMIC STRATEGIES FOR CONTROL OF HERPESVIRUSES OF POULTRY
Appropriated (D)
  Accession Number: 422253

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Reconstitution of infectious laryngotracheitis from a collection of overlapping cosmid clones - (Proceedings) - (07-Dec-14)
Development of a non invasion real-time PCR assay for the quantitation of chicken parvovirus in fecal swabs - (Proceedings) - (20-Nov-14)
Newcastle disease virus (NDV) recombinants expressing infectious laryngotracheitis virus (ILTV) glycoproteins gB and gD protect chickens against ILTV and NDV challenges Reprint Icon - (Peer Reviewed Journal)
Zhao, W., Spatz, S.J., Zhang, Z., Wen, G., Garcia, M., Zsak, L., Yu, Q. 2014. Newcastle disease virus (NDV) recombinants expressing infectious laryngotracheitis virus (ILTV) glycoproteins gB and gD protect chickens against ILTV and NDV challenges. Journal of Virology. 88(15):8397-8406. DOI: 10.1128/JVI.01321-14.
Newcastle disease virus as a vaccine vector for infectious laryngotracheitis - (Proceedings)
Spatz, S.J., Yu, Q., Zsak, L., Garcia, M. 2014. Newcastle disease virus as a vaccine vector for infectious laryngotracheitis. In: Proceedings of the 10th International Symposium on Marek's Disease and Avian Herpesviruses, July 20-23, 2014, East Lansing, Michigan. p. 66.
Molecular characterization of the complete genome of falconid herpesvirus strain S-18 - (Peer Reviewed Journal)
Spatz, S.J., Volkening, J.D., Ross, T.A. 2014. Molecular characterization of the complete genome of falconid herpesvirus strain S-18. Virus Research. 188:109-121.
Development of a high throughput TaqMan assay for the detection of infectious laryngotracheitis virus in vector vaccinated chickens - (Proceedings)
Spatz, S.J., Garcia, M., Yu, Q., Zsak, L. 2014. Development of a high throughput TaqMan assay for the detection of infectious laryngotracheitis virus in vector vaccinated chickens. In: Proceedings of the American Association of Avian Pathologists meeting, July 25-29, 2014, Denver, Colorado. p. 885.
Sequence analysis of CVI988-based vaccine, pCVI988-699-2-RV that has undergone a reversion to virulence - (Proceedings)
Rojas-Amortegui, J., Dong, H., Katneni, U., Gaddamanugu, S., Tavlarides-Hontz, P., Parcells, M.S., Abdo, Z., Spatz, S.J. 2014. Sequence analysis of CVI988-based vaccine, pCVI988-699-2-RV that has undergone a reversion to virulence. In: Proceedings of the 10th International Symposium on Marek's Disease and Avian Herpesviruses, July 20-23, 2014, East Lansing, Michigan. p. 39.
In vivo selection of CVI988 based vaccine, pCVI988-699-2: characterization of its efficacy and safety - (Proceedings)
McDowell, E., Dong, H., Katneni, U., Gaddamanugu, S., Tavlarides-Hontz, P., Parcells, M.S., Shaikh, S.R., Spatz, S.J. 2014. In vivo selection of CVI988 based vaccine, pCVI988-699-2: characterization of its efficacy and safety. In: Proceedings of the 10th International Symposium on Marek's Disease and Avian Herpesviruses, July 20-23, 2014, East Lansing, Michigan. p. 65.
Molecular epidemiology of infectious laryngotracheitis: a review - (Peer Reviewed Journal)
Menendez, K.R., Garcia, M., Spatz, S.J., Tablante, N.L. 2014. Molecular epidemiology of infectious laryngotracheitis: a review. Avian Pathology. 43(2):108-117. DOI: 10.1080/03079457.2014.886004.
Expression of chicken parvovirus VP2 in chicken embryo fibroblasts requires codon optimization for production of naked DNA and vectored Meleagrid herpesvirus type 1 vaccines - (Peer Reviewed Journal)
Spatz, S.J., Volkening, J.D., Mullis, R.A., Li, F., Mercado, J.C., Zsak, L. 2013. Expression of chicken parvovirus VP2 in chicken embryo fibroblasts requires codon optimization for production of naked DNA and vectored meleagrid herpesvirus type 1 vaccines. Virus Genes. 47:259-267. DOI: 10.1007/s11262-013-0944-9.
Genomic sequence analysis of the United States infectious laryngotracheitis vaccine strains chicken embryo origin (CEO) and tissue culture origin (TCO) - (Peer Reviewed Journal)
Garcia, M., Volkening, J., Riblet, S., Spatz, S.J. 2013. Genomic sequence analysis of the United States infectious laryngotracheitis vaccine strains chicken embryo origin (CEO) and tissue culture origin (TCO). Virology. 440(1):64-74. DOI: 10.1016/j.virol.2013.02.007.
Comparative full genome sequence analysis of columbid herpesvirus-1 and falconid herpesvirus-1 - (Proceedings) - (01-Feb-13)
Understanding the role of ORF-C gene in the pathogenicity of infectious laryngotracheitis virus - (Proceedings) - (01-Feb-13)
Attenuation, transmission, and immunogenicity of an ORF-C gene deleted strain of infectious laryngotracheitis virus (ILTV) in specific pathogen free chickens - (Proceedings) - (01-Dec-12)
Identification and characterization of the genomic termini and cleavage/packaging signals of gallid herpesvirus 2 - (Peer Reviewed Journal)
Volkening, J.D., Spatz, S.J. 2013. Identification and characterization of the genomic termini and cleavage/packaging signals of gallid herpesvirus 2. Avian Diseases. 57(2):401-408.
Identification and characterization of the genomic termini and cleavage/packaging signals of gallid herpesvirus 2 - (Proceedings)
Spatz, S.J., Volkening, J. 2012. Identification and characterization of the genomic termini and cleavage/packaging signals of gallid herpesvirus 2. In: Proceedings of the 9th International Symposium on Marek's Disease and Avian Herpesviruses, June 24-28, 2012, Berlin, Germany. p.27.
Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States - (Peer Reviewed Journal)
Spatz, S.J., Keeler, C.L., Kutish, G.F., Riblet, S.M., Volkening, J.D., Zsak, L., Afonso, C.L., Mundt, E.S., Rock, D.L., Garcia, M. 2012. Comparative full genome analysis of four infectious laryngotracheitis virus (gallid herpesvirus-1) virulent isolates from the United States. Virus Genes. 44:273-85.
Thermal inactivation of avian viral pathogens in an effluent treatment system within a biosafety level 2 and 3 enhanced facility - (Peer Reviewed Journal)
Chmielewski, R.A., Day, J.M., Spatz, S.J., Yu, Q., Gast, R.K., Zsak, L., Swayne, D.E. 2011. Thermal inactivation of avian viral pathogens in an effluent treatment system within a biosafety level 2 and 3 enhanced facility. Applied Biosafety. 16(4):206-217.
Development of a transfer plasmid for expression of foreign genes in meleagrid herpesvirus type 1 - (Proceedings)
Spatz, S.J., Zsak, L. 2011. Development of a transfer plasmid for expression of foreign genes in meleagrid herpesvirus type 1. American Association of Avian Pathologists. 1(1):237.
Genotypic characterization of two bacterial artificial chromosome clones derived from a single DNA source of the very virulent gallid herpesvirus-2 strain C12/130 - (Peer Reviewed Journal)
Spatz, S.J., Smith, L.P., Baigent, S.J., Petherbridge, L., Nair, V. 2011. Genotypic characterization of two bacterial artificial chromosome clones derived from a single DNA source of the very virulent gallid herpesvirus-2 strain C12/130. Journal of General Virology. 92:1500-1507
Comparative genomic sequence analysis of the Marek’s disease vaccine strain SB-1 - (Peer Reviewed Journal)
Spatz, S.J., Schat, K.A. 2011. Comparative genomic sequence analysis of the Marek’s disease vaccine strain SB-1. Virus Genes. 42:331-338.
Nucleotide sequence discrepancies within the GA strain of Marek's disease virus - (Proceedings)
Spatz, S.J., Lee, L.F., Silva, R.F. 2011. Nucleotide sequence discrepancies within the GA strain of Marek's disease virus. Western Poultry Disease Conference. 134-139.
Comparative genomic sequence analysis of the Marek’s disease vaccine strain SB-1 - (Proceedings)
Spatz, S.J., Karel, S. 2010. Comparative genomic sequence analysis of the Marek's desease vaccine strain SB-1. International Marek's Disease Symposium Abstracts and Proceedings. 1(1):141.
Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus - (Proceedings)
Spatz, S.J., Afonso, C.L., Zsak, L., Garcia, M., Mundt, E., Riblet, S., Keeler, C., Rock, D. 2010. Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus. Workshop on Molecular Pathogenesis of Marek's Disease and Avian Immunology. 1(1): 59.
Comparative nucleotide sequence analysis of three virulent strains of infectious laryngotracheitis virus (ILTV) - (Proceedings) - (21-Jun-10)
Comparative genomic sequence analysis of the Marek’s disease vaccine SB-1 - (Proceedings)
Spatz, S.J., Schat, K. 2010. Comparative genomic sequence analysis of the Marek’s disease vaccine SB-1. Herpesvirus International Workshop. 1(1):9-32.
Accumulation of attenuating mutations in varying proportions within a high passage very virulent plus strain of gallid herpesvirus type 2. - (Peer Reviewed Journal)
Spatz, S.J. 2010. Accumulation of attenuating mutations in varying proportions within a high passage very virulent plus strain of gallid herpesvirus type 2.Virus Res 149(2):135-42.
The genomes of Marek’s disease virus exist as quasispecies at defined intervals during serial passage-induced attenuation - (Proceedings)
Spatz, S.J., Gimeno, I.M., Witter, R.L., Heidari, M. 2010. The genomes of Marek’s disease virus exist as quasispecies at defined intervals during serial passage-induced attenuation. Western Poultry Disease Conference. 1(1):90-91.
Evolutionary Genomics of Marek's Disease Virus - (Proceedings)
Spatz, S.J. 2009. Evolutionary Genomics of Marek's Disease Virus. Meeting Proceedings. 1(1):8.
Cell surface display of highly pathogenic avian influenza hemagglutinin on the surface of Pichia pastoris cells using alpha-agglutinin for production of oral vaccines - (Peer Reviewed Journal)
Wasilenko, J.L., Sarmento, L., Spatz, S.J., Pantin Jackwood, M.J. 2010. Cell surface display of highly pathogenic avian influenza hemagglutinin on the surface of Pichia pastoris cells using alpha-agglutinin for production of oral vaccines. Biotechnology Progress. 26(2):542-547.
The genomes of Marek’s disease virus exist as quasispecies at defined intervals during serial passage-induced attenuation - (Proceedings)
Spatz, S.J., Gimeno, I.M., Heidari, M. 2009. The genomes of Marek’s disease virus exist as quasispecies at defined intervals during serial passage-induced attenuation. Herpesvirus International Workshop. 1(1):6-32.
Accumulation of quasi-species during serial passage-induced attenuation of Marek's Disease Virus-1 (MDV-1) - (Proceedings)
Spatz, S.J., Gimeno, I.M., Heidari, M. 2009. Accumulation of quasi-species during serial passage-induced attenuation of Marek's Disease Virus-1 (MDV-1). American Association of Avian Pathologists. 1(1): 128.
Vaccination of chickens against avian influenza using yeast cell surface display of H5 hemagglutinin - (Abstract Only)
Wasilenko, J.L., Pantin Jackwood, M.J., Sarmento, L., Suarez, D.L., Swayne, D.E., Spatz, S.J. 2009. Vaccination of chickens against avian influenza using yeast cell surface display of H5 hemagglutinin [abstract]. 7th International Symposium on Avian Influenza, April 5-9, 2009, Athens, Georgia, p. 60.
Purification of Marek's disease virus DNA for 454 pyrosequencing using micrococcal nuclease digestion and polyethylene glycol precipitation - (Peer Reviewed Journal)
Volkening, J.D., Spatz, S.J. 2009. Purification of DNA from the cell-associated herpesvirus Marek's disease virus for 454 pyrosequencing using micrococcal nuclease digestion and polyethylene glycol precipitation. Journal of Virological Methods. 157(1):55-61.
Identification of Marek's disease virus genes mutated during serial passage-induced attenuation. - (Proceedings)
Spatz, S.J., Gimeno, I.M., Heidari, M. 2008. Identification of Marek's disease virus genes mutated during serial passage-induced attenuation.. Meeting Proceedings. 1(1): 188.
Clustering of mutations within the inverted repeat regions of a serially-passaged attenuated gallid herpesvirus type 2 strain. - (Peer Reviewed Journal)
Spatz, S.J., Rue, C.A., Schumacher, D., Osterrieder, N. 2008. Clustering of mutations within the inverted repeat regions of a serially-passaged attenuated gallid herpesvirus type 2 strain. Virus Genes. 37:69-80.
High titer growth of human and avian influenza viruses in an immortalized chick embryo cell line without the need for exogenous proteases - (Peer Reviewed Journal)
Smith, K.A., Colvin, C.L., Weber, P.S., Spatz, S.J., Coussens, P.M. 2008. High titer growth of human and avian influenza viruses in an immortalized chick embryo cell line without the need for exogenous proteases. Vaccine. 26:3778-3782.
Marek’s disease virus genomics - (Proceedings)
Spatz, S.J. 2008. Marek’s disease virus genomics. International Marek's Disease Symposium Abstracts and Proceedings. 1(1):14.
Sequence determination of a mildly virulent strain (CU-2) of Gallid herpesvirus type 2 using 454 pyrosequencing - (Peer Reviewed Journal)
Spatz, S.J., Rue, C.A. 2008. Sequence determination of a mildly virulent strain (CU-2) of Gallid herpesvirus type 2 using 454 pyrosequencing. Virus Genes. 36:479-489.
Characterization of the a-like sequences of attenuated and nonattenuated strains of Marek's disease virus - (Proceedings)
Spatz, S.J., Rue, C.A. 2007. Characterization of the a-like sequences of attenuated and nonattenuated strains of Marek's disease virus. Research Workers in Animal Diseases Conference Proceedings. p. 115.
Research update on exotic and emerging poultry diseases - (Proceedings)
Pantin Jackwood, M.J., Suarez, D.L., Zsak, L., Spackman, E., Kapczynski, D.R., King, D.J., Afonso, C.L., Day, J.M., Spatz, S.J., Yu, Q., Swayne, D.E. 2007. Research update on exotic and emerging poultry diseases. In: Proceedings of the 111th Annual Meeting of the U. S. Animal Health Association, Reno, Nevada, October 22-23, 2007. p. 702-707. Available: http://www.usaha.org/meetings/2007/2007_USAHA_Proceedings.pdf.
Horizontal transmission of Marek’s disease virus requires both the unique-long (UL) 13 protein kinase (UL13) and the UL44 glycoprotein C - (Peer Reviewed Journal)
Jarosinski, K.W., Margulis, N.G., Kamil, J.P., Spatz, S.J., Nair, V.K., Osterrieder, N. 2007. Horizontal transmission of Marek’s disease virus requires US2, the UL13 protein kinase, and gC. Journal of Virology. Oct;81(19):10575-87.
Comparative sequence analysis of a highly oncogenic but horizontal spread-defective clone of Marek's disease virus - (Peer Reviewed Journal)
Spatz, S.J., Zhao, Y., Petherbridge, L., Smith, L., Baigent, S., Nair, V. 2007. Comparative sequence analysis of a highly oncogenic but horizontal spread-defective clone of Marek's disease virus. Virus Genes. 35:753-766.
Comparative sequence analysis of a highly oncogenic but horizontal spread-deficient clone of Marek's disease virus - (Proceedings)
Spatz, S.J., Zhao, Y., Petherbridge, L., Smith, L.P., Baigent, S.J., Nair, V. 2007. Comparative sequence analysis of a highly oncogenic but horizontal spread-deficient clone of Marek's disease virus [abstract]. 32nd Annual International Herpesvirus International Workshop. Paper No. 6.24.
Characterization of the a-like sequences of attenuated and nonattenuated strains of Marek's Disease Virus 1 (MDV-1) - (Proceedings)
Rue, C.A., Spatz, S.J. 2007. Characterization of the a-like sequences of attenuated and nonattenuated strains of Marek's Disease Virus 1 (MDV-1).[abstract]. Herpesvirus International Workshop. Poster 6.24
Sequence determination of variable regions within the genomes of Gallid herpesvirus-2 pathotypes - (Peer Reviewed Journal)
Spatz, S.J., Silva, R.F. 2007. Sequence determination of variable regions within the genomes of Gallid herpesvirus-2 pathotypes. Archives of Virology. Available: http://www.springerlink.com/content/ku4064776312w861/.
Comparative Full Length Sequence Analysis of Oncogenic and Vaccine (Rispens) Strains of Marek's Disease Virus - (Abstract Only)
Spatz, S.J., Petherbridge, L., Zhao, Y., Nair, V. 2006. Comparative Full Length Sequence Analysis of Oncogenic and Vaccine (Rispens) Strains of Marek's Disease Virus. Proceedings of 87th Conference for Research Workers in Animal Diseases. p. 166.
Comparative full-length sequence analysis of oncogenic and vaccine (Rispens) strains of Marek's disease virus - (Peer Reviewed Journal)
Spatz, S.J., Nair, V. 2007. Comparative full-length sequence analysis of oncogenic and vaccine (Rispens) strains of Marek's disease virus. Journal of General Virology. 88:1080-1096.
Global Gene Expression Profiling During Cytolytic Infection of Marek's Disease Virus: Array Analysis of Host-Pathogen Interactions - (Abstract Only)
Heidari, M., Huebner, M., Zhang, H.M., Silva, R.F., Spatz, S.J., Kireev, D. 2006. Global gene expression profiling during cytolytic infection of Marek's disease virus: array analysis of host-pathogen interactions [abstract]. 4th International Workshop on Molecular Pathogenesis of Marek's Disease Virus. p. 8.
Role of Ul13 in Generating Cell-Free Mdv - (Abstract Only)
Silva, R.F., Lee, L.F., Heidari, M., Gimeno, I.M., Spatz, S.J., Zelepsky, J. 2006. The role of UL13 in generating cell-free MDV [abstract]. 4th International Workshop on Molecular Pathogenesis of Marek's Disease Virus. p. 28.
Polymorphisms in the Long Repeat Regions of Oncogenic and Attenuated Pathotypes of Marek's Disease Virus 1 - (Peer Reviewed Journal)
Spatz, S.J., Silva, R.F. 2007. Polymorphisms in the long repeat regions of oncogenic and attenuated pathotypes of Marek's disease virus 1. Virus Genes. 35(1):41-53.
Polymorphisms in the Genomes of Oncogenic and Attenuated Pathotypes of Marek's Disease Virus Serotype 1 - (Abstract Only)
Spatz, S.J., Nair, V. 2006. Polymorphisms in the genomes of oncogenic and attenuated pathotypes of marek's disease virus serotype 1. American Veterinary Medical Association, July 20-22, 2006, Honolulu, HI. 2006 CDROM.
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Last Modified: 12/22/2014
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