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Spencer A Leigh
Poultry Research
Research Microbiologist

Phone: (662) 320-7607
Fax:

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Projects
Control Strategies for Avian Mycoplasmosis
In-House Appropriated (D)
  Accession Number: 441226
Intervention Strategies to Mitigate Avian Escherichia coli Infections and Antimicrobial Resistance in the Poultry Environment
In-House Appropriated (D)
  Accession Number: 441425
Identification of the Molecular Determinants of Pathogenesis, Strain Variation, and Tissue Tropism of Different E. coli Strains
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437284

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Effects of the in ovo injection of an Escherichia coli vaccine on the hatchability and quality characteristics of commercial layer hatchlings Reprint Icon - (Peer Reviewed Journal)
Whole-Genome sequencing of three M. gallisepticum isolates similar to the 6/85 vaccine strain Reprint Icon - (Peer Reviewed Journal)
Leigh, S.A., Evans, J.D. 2023. Whole-Genome sequencing of three M. gallisepticum isolates similar to the 6/85 vaccine strain. Microbiology Resource Announcements. https://doi.org/10.1128/mra.00339-23.
Effects of the In ovo vaccination of the ts-11 strain of mycoplasma gallisepticum in layer embryos and posthatch chicks Reprint Icon - (Peer Reviewed Journal)
Alqhtani, A.H., Fatemi, S.A., Collins Elliott, K.E., Branton, S.L., Evans, J.D., Leigh, S.A., Gerard, P.D., Peebles, E.D. 2022. Effects of the In ovo vaccination of the ts-11 strain of mycoplasma gallisepticum in layer embryos and posthatch chicks. Animals. 2022,12,1120. https://doi.org/10.3390/ani12091120.
Effects of dietary Original XPC™ on selected blood variables in layer pullets challenged 3 with Mycoplasma gallisepticum Reprint Icon - (Peer Reviewed Journal)
Peebles, E.D., Elliott, K., Branton, S.L., Evans, J.D., Leigh, S.A., Kim, E.J., Olanrewaju, H.A., Pharr, G.T., Pavlidis, H.O., Gerard, P.D. 2020. Effects of dietary Original XPC™ on selected blood variables in layer pullets challenged 3 with Mycoplasma gallisepticum. Poultry Science. 99:4373-4383. https://doi.org/10.1016/j.psj.2020.06.005.
Growth and humoral immune effects of dietary Original XPC™ in layer pullets challenged with Mycoplasma gallisepticum Reprint Icon - (Peer Reviewed Journal)
Elliott, K.C., Branton, S.L., Evans, J.D., Leigh, S.A., Kim, E.J., Olanrewaju, H.A., Pharr, G.T., Pavlidis, H.O., Gerard, P.D., Peebles, E.D. 2020. Growth and humoral immune effects of dietary Original XPC™ in layer pullets challenged with Mycoplasma gallisepticum. Poultry Science. 99(6):3030-3037.
Effects of different vaccine combinations against Mycoplasma gallisepticum on the digestive and reproductive organ characteristics of commercial egg-laying hens - (Peer Reviewed Journal)
Peebles, E.D., Jacob, R., Branton, S.L., Evans, J.D., Leigh, S.A., Gerard, P.D. 2015. Effects of different vaccine combinations against Mycoplasma gallisepticum on the digestive and reproductive organ characteristics of commercial egg-laying hens. Poultry Science. 94:2898-2904.
Effects of different vaccine combinations against Mycoplasma gallisepticum on blood characteristics in commercial layer chickens - (Peer Reviewed Journal)
Peebles, E.D., Jacobs, R., Branton, S.L., Evans, J.D., Leigh, S.A., Gerard, P.D. 2015. Effects of different vaccine combinations against Mycoplasma gallisepticum on blood characteristics in commercial layer chickens. Poultry Science. 94:2108-2113.
The impact of deposition site on vaccination efficiency of a bacterial-based poultry vaccine - (Peer Reviewed Journal)
Effects of different vaccine combinations against Mycoplasma gallisepticum on the internal egg and eggshell characteristics of commercial layer chickens - (Peer Reviewed Journal)
Jacob, R., Branton, S.L., Evans, J.D., Leigh, S.A., Peebles, E.D. 2015. Effects of different vaccine combinations against Mycoplasma gallisepticum on the internal egg and eggshell characteristics of commercial layer chickens. Poultry Science. 94:912-917.
Proteomic analysis of Mycoplasma gallisepticum vaccine strain F - (Peer Reviewed Journal)
Collier, S.D., Branton, S.L., Evans, J.D., Leigh, S.A., Wan, X., Cooksey, A.M., Olanrewaju, H.A., Pharr, G.T. 2015. Proteomic analysis of Mycoplasma gallisepticum vaccine strain F. International Journal of Poultry Science. 14(1): 1-12.
Stability of rehydrated Mycoplasma gallisepticum vaccine homogeneity over time - (Peer Reviewed Journal)
Leigh, S.A., Evans, J.D., Branton, S.L. 2014. Stability of rehydrated Mycoplasma gallisepticum vaccine homogeneity over time. International Journal of Poultry Science. 13(10):549-551.
Effects of live and killed vaccines against Mycoplasma gallisepticum on the performance characteristics of commercial layer chickens - (Peer Reviewed Journal)
Jacob, R., Branton, S.L., Evans, J.D., Leigh, S.A., Peebles, E.D. 2014. Effects of live and killed vaccines against Mycoplasma gallisepticum on the performance characteristics of commercial layer chickens. Poultry Science. 93:1403-1409.
Impact of fowlpox-vectored Mycoplasma gallisepticum vaccine Vectormune® FP MG on layer hen egg production and egg quality parameters - (Peer Reviewed Journal)
Leigh, S.A., Branton, S.L., Evans, J.D., Collier, S.D. 2013. Impact of fowlpox-vectored Mycoplasma gallisepticum vaccine Vectormune® FP MG on layer hen egg production and egg quality parameters. Poultry Science. 92(12):3172-3175.
Spray application of live attenuated F Strain-derived Mycoplasma gallisepticum vaccines - (Peer Reviewed Journal)
Evans, J.D., Jacob, R., Leigh, S.A., Collier, S.D., Peebles, E.D., Branton, S.L. 2013. Spray application of live attenuated F Strain-derived Mycoplasma gallisepticum vaccines. Journal of Applied Poultry Research. 22:842-848.
Mycoplasma gallisepticum transmission: Comparison of commercial F-strain vaccine versus layer complex-derived field strains in a tunnel ventilated house - (Peer Reviewed Journal)
Purswell, J.L., Evans, J.D., Leigh, S.A., Collier, S.D., Olanrewaju, H.A., Kim, E.J., Pharr, G.T., Peebles, E.D., Branton, S.L. 2012. Mycoplasma gallisepticum transmission: Comparison of commercial F-strain vaccine versus layer complex-derived field strains in a tunnel ventilated house. Poultry Science. 91:3072-3079.
Effect of infection route and concurrent infectious bronchitis virus vaccination on Mycoplasma gallisepticum disease pathology in an experimental model - (Peer Reviewed Journal)
Leigh, S.A., Branton, S.L., Evans, J.D., Collier, S.D. 2012. Effect of infection route and concurrent infectious bronchitis virus vaccination on Mycoplasma gallisepticum disease pathology in an experimental model. Avian Pathology. 41(5):497-503.
A comparative study of live attenuated F strain-derived Mycoplasma gallisepticum vaccines - (Peer Reviewed Journal)
Evans, J.D., Leigh, S.A., Purswell, J.L., Jacob, R., Peebles, E.D., Collier, S.D., Branton, S.L. 2012. A comparative study of live attenuated F strain-derived Mycoplasma gallisepticum vaccines. Avian Diseases. 56:396-401.
Analysis of the effect of diluent for rehydration of PoulVac MycoF on vaccination seroconversion results - (Peer Reviewed Journal)
Leigh, S.A., Branton, S.L., Collier, S.D., Evans, J.D. 2011. Analysis of the effect of diluent for rehydration of PoulVac MycoF on vaccination seroconversion results. International Journal of Poultry Science. 10(5):397-400.
A chronicle of serologic response in commercial layer chickens to vaccination with commercial F strain Mycoplasma gallisepticum vaccine - (Peer Reviewed Journal)
Branton, S.L., Leigh, S.A., Purswell, J.L., Evans, J.D., Collier, S.D., Olanrewaju, H.A., Pharr, G.T. 2010. A chronicle of serologic response in commercial layer chickens to vaccination with commercial F strain Mycoplasma gallisepticum vaccine. Avian Diseases. 54(3):1108-1111.
Proteomics inference of genes involved in host adaptation of Mycoplasma gallinarum - (Peer Reviewed Journal)
Wan, X., Branton, S.L., Collier, S.D., Evans, J.D., Leigh, S.A., Pharr, G.T. 2010. Proteomics inference of genes involved in host adaptation of Mycoplasma gallinarum. Veterinary Microbiology. 145(1-2):177-184.
Effects of vaccination with F-strain Mycoplasma gallisepticum on egg production and quality parameters of commercial layer hens previously vaccinated with 6/85-strain Mycoplasma gallisepticum - (Peer Reviewed Journal)
Leigh, S.A., Branton, S.L., Evans, J.D., Collier, S.D., Peebles, E.D. 2010. Effects of vaccination with F-strain Mycoplasma gallisepticum on egg production and quality parameters of commercial layer hens previously vaccinated with 6/85-strain Mycoplasma gallisepticum. Poultry Science. 89(3):501-504.
Effect of Dosage and Vaccination Route on Transmission of a Live Attenuated Mycoplasma gallesepticum Vaccine: A Broiler Model - (Peer Reviewed Journal)
Evans, J.D., Branton, S.L. 2009. Effect of Dosage and Vaccination Route on Transmission of a Live Attenuated Mycoplasma gallesepticum Vaccine. Avian Diseases. 53(3):416-420.
Detection of Mycoplasma gallinarum by real-time PCR - (Peer Reviewed Journal)
Leigh, S.A., Evans, J.D. 2009. Detection of Mycoplasma gallinarum by real-time PCR. International Journal of Poultry Science 8(2):108-111.
Recent Advances in Mycoplasma gallisepticum Vaccine Administration - (Popular Publication)
Branton, S.L., Leigh, S.A., Purswell, J.L. 2008. Recent Advances in Mycoplasma gallisepticum Vaccine Administration. Feed and Livestock Magazine 5(4):28-31.
Effects of System Pressure and Nozzle Type on Spray Application of Avian Vaccines - (Peer Reviewed Journal)
Purswell, J.L., Fritz, B.K., Branton, S.L., Leigh, S.A. 2008. Effects of System Pressure and Nozzle Type on Spray Application of Avian Vaccines. Applied Engineering in Agriculture 24(6):785-789.
Differentiation of Mycoplasma gallisepticum vaccine strains ts-11 and 6/85 from commonly used Mycoplasma gallisepticum challenge strains by PCR - (Peer Reviewed Journal)
Evans, J.D., Leigh, S.A. 2008. Differentiation of Mycoplasma gallisepticum vaccine strains ts-11 and 6/85 from commonly used Mycoplasma gallisepticum challenge strains by PCR. Avian Dis. 52(3):491-497.
Stabilization of live Mycoplasma gallisepticum vaccines during vaccination with second generation Spray-Vac® vaccine stabilizer - (Peer Reviewed Journal)
Leigh, S.A., Branton, S.L., Collier, S.D. 2008. Stabilization of live Mycoplasma gallisepticum vaccines during vaccination with second generation Spray-Vac® vaccine stabilizer. Journal of Applied Poultry Research 17:278-282.
Effect of selected water temperatures used in Mycoplasma gallisepticum vaccine reconstitution on titer at selected time intervals - (Peer Reviewed Journal)
Branton, S.L., Leigh, S.A., Roush, W.B., Purswell, J.L., Olanrewaju, H.A., Collier, S.D. 2008. Effect of selected water temperatures used in Mycoplasma gallisepticum vaccine reconstitution on color change units at selected time intervals. Avian Diseases. 52:291-296.
The effects of increasing sodium chloride concentration on Mycoplasma gallisepticum vaccine survival in solution. - (Peer Reviewed Journal)
Leigh, S.A., Evans, J.D., Branton, S.L., Collier, S.D. 2008. The effects of increasing sodium chloride concentration on Mycoplasma gallisepticum vaccine survival in solution.. Avian Diseases. 52:136-138.
Effects of increased dosages of the Mycoplasma gallisepticum vaccine MYCOVAC-L® in layer chickens subsequently challenged with virulent M. gallisepticum: egg production and serologic response - (Peer Reviewed Journal)
Evans, J.D., Leigh, S.A., Branton, S.L., Collier, S.D. 2007. Effects of increased dosages of the Mycoplasma gallisepticum vaccine MYCOVAC-L® in layer chickens subsequently challenged with virulent M. gallisepticum: egg production and serologic response. Avian Diseases 51:912-917.
INITIAL PROTEOMIC ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEINS FROM MYCOPLASMA GALLISEPTICUM VACCINE STRAINS TS-11 AND F DETECTED BY WESTERN BLOTTING - (Peer Reviewed Journal)
Collier, S. D., Pharr, G. T., Branton, S. L., Evans, J. D., Leigh, S. A., and Felfoldi, B. 2006. Initial Proteomic Analysis of Differentially Expressed Proteins from Mycoplasma gallisepticum Vaccine Strains ts-11 and F Detected by Western Blotting. Int. J. of Poultry Sci. 5(4): 330-336.
PROTEOMIC ANALYSIS OF MYCOPLASMA GALLISEPTICUM VACCINE STRAIN F - (Abstract Only)
MYCOPLASMA GALLISEPTICUM: CURRENT AND DEVELOPING MEANS TO CONTROL THE AVIAN PATHOGEN - (Peer Reviewed Journal)
Evans, J.D., Leigh, S.A., Branton, S.L., Collier, S.D., Pharr, G.T., Bearson, S.M. 2005. Mycoplasma gallisepticum: current and developing means to control the avian pathogen. Journal of Applied Poultry Research. 14:757-763.