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United States Department of Agriculture

Agricultural Research Service

Luisa Wai Wai Cheng
Foodborne Toxin Detection and Prevention
Acting Research Leader

Phone: (510) 559-6337
Fax: (510) 559-6429
USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY, CA, 94710

Projects
Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins
Appropriated (D)
  Accession Number: 421099
Development of Detection Technologies for Bacterial Neurotoxins and Their Validation in Food Matrices
Specific Cooperative Agreement (S)
  Accession Number: 422443
Toxin Detection with the SpinDx Microfluidics Detection Technology
Specific Cooperative Agreement (S)
  Accession Number: 426477

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Beta-carotene encapsulated in food protein nanoparticles reduces peroxyl radical oxidation in Caco-2 cells - (Peer Reviewed Journal) - (24-Apr-14)
Cellular uptake of beta-carotene from protein stabilized solid lipid nano-particles prepared by homogenization-evaporation method - (Peer Reviewed Journal)
Yi, J., Lam, T.I., Yokoyama, W.H., Cheng, L.W., Zhong, F. 2014. Cellular uptake of beta-carotene from protein stabilized solid lipid nano-particles prepared by homogenization-evaporation method. Journal of Agricultural and Food Chemistry. 62(5):1096-104.
A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food - (Peer Reviewed Journal)
Stanker, L.H., Scotcher, M.C., Cheng, L.W., Ching, K., McGarvey, J.A., Hodge, D., Hnasko, R.M. 2013. A monoclonal antibody based capture ELISA for botulinum neurotoxin serotype B: toxin detection in food. Toxins. 5(11):2212-2226. DOI:10.3390/toxins5112212.
Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies - (Peer Reviewed Journal)
Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity - (Peer Reviewed Journal)
Lee, K., Gu, S., Jin, L., Le, T., Cheng, L.W., Strotmeier, J., Krue, A.M., Perry, K., Yao, G., Rummel, A., Jin, R. 2013. Structure of a bimodular botulinum neurotoxin complex provides insights into its oral toxicity. PLoS Pathogens. 9(10):e1003690. DOI: 10.1371/journal.ppat.1003690.
Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples - (Peer Reviewed Journal)
Bagramyan, K., Kaplan, B.E., Cheng, L.W., Rummel, A., Kalkum, M. 2013. Substrates and controls for the quantitative detection of active botulinum neurotoxin in protease-containing samples. Analytical Chemistry. 85(11):5569-76. doi: 10.1021/ac4008418.
Translocation of botulinum neurotoxins and associated proteins across intestinal epithelial cells(Abstract) - (Abstract Only) - (16-Mar-13)
Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum - (Peer Reviewed Journal)
Cheng, L.W., Stanker, L.H. 2013. Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum. Journal of Agricultural and Food Chemistry. 61(3):755-760 doi:10.1021/jf3041963.
Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA - (Peer Reviewed Journal)
Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.
Monoclonal antibodies and reagents for botulinum research - (Proceedings)
Stanker, L.H., Cheng, L.W. 2012. Monoclonal antibodies and reagents for botulinum research. The Botulinum Journal. 2(2):150-155 doi: 10.1504/tbj2012.050197.
Replacement of animals in assays for detection of toxins in food(Abstract) - (Abstract Only) - (10-Jul-12)
Detection of botulinum neurotoxin serotypes A and B using chemiluminescence and electrochemiluninescene immunoassays in food and serum matrices - (Abstract Only) - (21-Dec-11)
Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples - (Book / Chapter)
Cheng, L.W., Land, K.M., Stanker, L.H. 2012. Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples. Morse, S., editor. Bioterrorism. Rijeka, Croatia: Intech. p. 1-16.
Comparison of oral toxicological properties of botulinum neurotoxin - (Peer Reviewed Journal)
Cheng, L.W., Henderson II, T.D. 2011. Comparison of oral toxicological properties of botulinum neurotoxin. Toxicon. 58:62-67.
Botulinum neurotoxin: a deadly protease with applications to human medicine - (Book / Chapter)
Land, K.M., Cheng, L.W. 2010. Botulinum neurotoxin: a deadly protease with applications to human medicine. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Spain. Formatex Research Center. 965-971.
Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.
Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and its Application to Toxin Detection in Milk - (Peer Reviewed Journal)
Scotcher, M.C., Cheng, L.W., Stanker, L.H. 2010. Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and its Application to Toxin Detection in Milk. PLos One. 5(6):e11047.doi:10.1371/journal.pone.0011047.
Comparison of Toxicological Properties of Botulinum Neurotoxin Serotypes A and B in Mice - (Abstract Only)
Cheng, L.W., Henderson Ii, T.D., Scotcher, M.C., Stanker, L.H. 2009. Comparison of Toxicological Properties of Botulinum Neurotoxin Serotypes A and B in Mice. American Chemical Society [Abstract]. Paper No. 26541
Antibody Protection Against Botulinum Neurotoxin Intoxication In Mice - (Peer Reviewed Journal)
Cheng, L.W., Stanker, L.H., Henderson Ii, T.D., Lou, J., Marks, J.D. 2009. Antibody Protection Against Botulinum Neurotoxin Intoxication In Mice. Infection and Immunity. 77(10):4305-4313.
Detection of Protein Toxins - (Abstract Only)
Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.
Botulinum Neurotoxin Research at the Western Regional Research Center - (Abstract Only) - (09-Apr-09)
Influence of Gene Expression on Variable Aflatoxin Production by Different Strains of Aspergillus flavus - (Abstract Only) - (01-Feb-09)
Botox and Our Tax Dollars at Work - (Other) - (06-Oct-08)
Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods - (Proceedings)
Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.
Effect of food matrices on the biological activity of ricin - (Peer Reviewed Journal)
He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.
Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay - (Peer Reviewed Journal)
Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139
Effects of purification on the bioavailability of botulinum neurotoxin type A - (Peer Reviewed Journal)
Cheng, L.W., Onisko, B.C., Johnson, E.A., Reader, J.R., Griffey, S.M., Larson, A.E., Tepp, W.H., Stanker, L.H., Brandon, D.L., Carter, J.M. 2008. Effects of purification on the bioavailability of botulinum neurotoxin type A. Toxicology. 249(2008):123-129.
Development and Partial Characterization of High-affinity Monoclonal Antibodies for Botulinum Toxin Type A and their use in Analysis of Milk by Sandwich ELISA - (Peer Reviewed Journal)
Stanker, L.H., Merrill, P.A., Scotcher, M.C., Cheng, L.W. 2008. Development and Partial Characterization of High-affinity Monoclonal Antibodies for Botulinum Toxin Type A and their use in Analysis of Milk by Sandwich ELISA. Journal of Immunological Methods, 336:1-8.
Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs - (Peer Reviewed Journal)
He, X., Carter, J.M., Brandon, D.L., Cheng, L.W., Mckeon, T.A. 2007. Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry. 55:6897-6902
Molecular Approaches for the Identification of Toxin in Foods - (Abstract Only) - (23-Feb-07)
Bioavailability of Crude Ricin in Food Matrices - (Abstract Only)
Cheng, L.W., Brandon, D.L., He, X., Chen, G.Q., Carter, J.M. 2006. Bioavailability of crude ricin in food matrices. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 64
Development of Monoclonal Antibodies Specific for Ricin - (Abstract Only)
Brandon, D.L., Cheng, L.W., He, X., Carter, J.M. 2006. Development of monoclonal antibodies specific for ricin. [Abstract]. ACS National Meeting and Exposition. Oral Presentation AGRO 234
Development of New Monoclonal Antibodies Specific for Botulinum Neurotoxin Type A - (Abstract Only)
Stanker, L.H., Merrill, P.A., Cheng, L.W., Carter, J.M., Brandon, D.L. 2006. Development of new monoclonal antibodies specific for botulinum neurotoxin type a. [Abstract]. ACS National Meeting. Oral Presentation AGRO 235.
ARS News Articles
Last Modified: 10/30/2014
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