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United States Department of Agriculture

Agricultural Research Service

Brian Shepherd
U.S. Dairy Forage Research Center
Research Physiologist

Phone: (414) 382-1767
Fax: (414) 382-1705

1925 LINDEN DR W
MADISON, WI, 53706

Projects
COMBATING VIRAL HEMORRHAGIC SEPTICEMIA AND IMPROVING YELLOW PERCH AQUACULTURE FOR THE GREAT LAKES REGION
Appropriated (D)
  Accession Number: 420493
Characterizing Pathogenicity of Great Lakes Strains of VHSV and Developing Tools to Study the Influence of Stress on Immune Function in Yellow Perch
Specific Cooperative Agreement (S)
  Accession Number: 427467
Improving and Preserving Yellow Perch Genetic Resources
Specific Cooperative Agreement (S)
  Accession Number: 427524

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Identification of gender in yellow perch Perca flavescens using external morphology - (Abstract Only)
Shepherd, B.S., Rees, C.B., Sepulveda Villet, O.J., Palmquist, D.E., Binkowski, F.P. 2014. Identification of gender in yellow perch Perca flavescens using external morphology. Book of Abstracts World Aquaculture Society. Paper No. 224.
Influence of mineral supplementation on growth in yellow perch Perca flavescens fed a soy-based diet - (Abstract Only)
Shepherd, B.S., Sepulveda Villet, O.J., Barrows, F., Sealey, W.M., Gaylord, T., Palmquist, D.E., Hill, H., Binkowski, F.P. 2014. Influence of mineral supplementation on growth in yellow perch Perca flavescens fed a soy-based diet. Book of Abstracts World Aquaculture Society. Paper No. 224.
Comparative effects of constant versus fluctuating thermal regimens on yellow perch growth, feed conversion and survival - (Abstract Only)
Fisher, G.J., Held, J.A., Malison, J.A., Hartleb, C.J., Holmes, K., Shepherd, B.S. 2014. Comparative effects of constant versus fluctuating thermal regimens on yellow perch growth, feed conversion and survival [abstract]. World Aquaculture Society. Paper No. 270.
Accurate detection and quantification of the fish viral hemorrhagic septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay - (Peer Reviewed Journal)
Pierce, L.R., Willey, J.C., Palsule, V.V., Yeo, J., Shepherd, B.S., Crawford, E.L., Stepien, C.A. 2013. Accurate detection and quantification of the fish viral hemorrhagic septicemia virus (VHSv) with a two-color fluorometric real-time PCR assay. PLoS One. 8(8): e71851.
Live feed enrichment with probiotics: current methods and future perspectives on increasing finfish larviculture success - (Trade Journal) - (04-Jun-13)
A new StaRT-PCR approach to detect and quantify fish viral hemorrhagic septicemia virus (VHSv): enhanced quality control with internal standards Reprint Icon - (Peer Reviewed Journal)
Pierce, L.R., Willey, J.C., Crawford, E.L., Palsule, V.V., Leaman, D.A., Faisal, M., Kim, R.K., Shepherd, B.S., Stanoszek, L.M., Stepien, C.A. 2013. A new StaRT-PCR approach to detect and quantify fish viral hemorrhagic septicemia virus (VHSv): enhanced quality control with internal standards. Journal of Virological Methods. 189:129-142.
Identification of gender in yellow perch by external morphology: validation in four geographic strains and effects of estradiol Reprint Icon - (Peer Reviewed Journal)
Shepherd, B.S., Rees, C.B., Sepulveda Villet, O.J., Palmquist, D.E., Binkowski, F.P. 2013. Identification of gender in yellow perch by external morphology: validation in four geographic strains and effects of estradiol. North American Journal of Aquaculture. 75: 361-372.
Status and prospects for the yellow perch genetic improvement program in Milwaukee, Wisconsin - (Abstract Only) - (29-Sep-12)
Expanding the evaluation of probiotics and prebiotics for aquafeeds: perspectives on the limitations and needs for surrogate measures of effectiveness - (Abstract Only) - (28-Sep-12)
Characterization and evaluation of sex-specific expression of suppressors of cytokine signaling (SOCS) -1 and -3 in juvenile yellow perch (Perca flavescens) treated with lipopolysaccharide Reprint Icon - (Peer Reviewed Journal)
Shepherd, B.S., Rees, C.B., Binkowski, F., Goetz, F. 2012. Characterization and evaluation of sex-specific expression of suppressors of cytokine signaling (SOCS) -1 and -3 in juvenile yellow perch (Perca flavescens) treated with lipopolysaccharide. Fish and Shellfish Immunology. 33:468-481.
Effects of dietary estrogen treatment on growth and feed intake in yellow perch (Perca flavescens) - (Abstract Only)
Shepherd, B.S., Rees, C.B., Groff, C.M., Goetz, F.W., Binkowski, F.P. 2012. Effects of dietary estrogen treatment on growth and feed intake in yellow perch (Perca flavescens). 10th International Congress on the Biology of Fish. p. 115.
Development of yellow perch (Perca flavescens) broodstocks: initial characterization of growth and quality traits following grow-out of different stocks Reprint Icon - (Peer Reviewed Journal)
Rosauer, D.R., Biga, P.R., Lindell, S., Binkowski, F., Shepherd, B.S., Palmquist, D.E., Simchick, C., Goetz, F.W. 2011. Development of yellow perch (Perca flavescens) broodstocks: initial characterization of growth and quality traits following grow-out of different stocks. Aquaculture. 317: 58-66.
Developmental expression and estrogen responses of endocrine genes in juvenile yellow perch (Perca flavescens) Reprint Icon - (Peer Reviewed Journal)
Lynn, S.G., Wallat, G.K., Malison, J.A., Shepherd, B.S. 2011. Developmental expression and estrogen responses of endocrine genes in juvenile yellow perch (Perca flavescens). General and Comparative Endocrinology. 171:151-159.
Acute handling disturbance modulates plasma insulin-like growth factor binding proteins in rainbow trout (Oncorhynchus mykiss) Reprint Icon - (Peer Reviewed Journal)
Shepherd, B.S., Aluru, N., Vijayan, M. 2011. Acute handling disturbance modulates plasma insulin-like growth factor binding proteins in rainbow trout (Oncorhynchus mykiss). Domestic Animal Endocrinology. 40:129-138.
Characterization and Regulation of Suppressor of Cytokine Signaling (SOCS) Genes in Yellow Perch (Perca flavescens) - (Abstract Only)
Shepherd, B.S., Rees, C.B., Goetz, F.W. 2010. Characterization and Regulation of Suppressor of Cytokine Signaling (SOCS) Genes in Yellow Perch (Perca flavescens) [abstract]. In: 2010 World Aquaculture Society Meeting, March 1-5, 2010, San Diego, California. 2010. CDROM (Abstract 909).
Development of Genetically Defined Yellow Perch (Perca flavescens) Broodstocks: Results of Performance Trial on F1 Generations - (Abstract Only)
Rosauer, D., Binkowski, F., Shepherd, B.S., Goetz, F. 2010. Development of Genetically Defined Yellow Perch (Perca flavescens) Broodstocks: Results of Performance Trial on F1 Generations [abstract]. In: 2010 World Aquaculture Society Meeting, March 1-5, 2010, San Diego, California. 2010. CDROM (Abstract 854).
Genetic Variation of 17 Wild Yellow Perch Populations from the Midwest and East Coast Analyzed Via Microsatellites Reprint Icon - (Peer Reviewed Journal)
Grzybowski, M., Sepulveda-Villet, O.J., Stepien, C.A., Rosauer, D., Binkowski, F., Klaper, R., Shepherd, B.S., Goetz, F. 2010. Genetic Variation of 17 Wild Yellow Perch Populations from the Midwest and East Coast Analyzed Via Microsatellites. Transactions of the American Fisheries Society. 139:270-287.
Stimulation of Growth and Changes in the Hepatic Transcriptome by Estradiol-17-Beta in the Yellow Perch (Perca flavescens) Reprint Icon - (Peer Reviewed Journal)
Goetz, F.W., Rise, M.L., Rise, M., Goetz, G.W., Binkowski, F.P., Shepherd, B.S. 2009. Stimulation of Growth and Changes in the Hepatic Transcriptome by Estradiol-17-Beta in the Yellow Perch (Perca flavescens). Physiological Genomics. Available: http://physiolgenomics.physiology.org/cgi/content/abstract/00069.2009v1.
Aquaculture in the 21st Century and Beyond - (Abstract Only)
Shepherd, B.S. 2009. Aquaculture in the 21st Century and Beyond [abstract]. 2009 Wisconsin Renewable Energy Summit, Water Technology, March 25-28, 2009, Milwaukee, Wisconsin. Session 18-3.
Seasonal and Sex-Specific mRNA Levels of Key Endocrine Genes in Adult Yellow Perch (Perca flavescens) from Lake Erie Reprint Icon - (Peer Reviewed Journal)
Lynn, S.G., Powell, K.A., Westneat, D.D., Shepherd, B.S. 2009. Seasonal and Sex-Specific mRNA Levels of Key Endocrine Genes in Adult Yellow Perch (Perca flavescens) from Lake Erie. Marine Biotechnology. 11:210-222.
Characterization of Genes Involved with Growth and Immunity in the Yellow Perch (Perca Flavescens) Reprint Icon - (Abstract Only)
Shepherd, B.S., Rees, C.B., Binkowski, F.P., Goetz, F.R. 2008. Characterization of genes involved with growth and immunity in the yellow perch (Perca flavescens). Abstract Book of Eighth International Congress on the Biology of Fish. p. 89.
Effects of short term growth hormone treatment on the rainbow trout (Oncorhynchus mykiss) liver and muscle transcriptomes - (Peer Reviewed Journal)
Gahr, S.A., Vallejo, R.L., Weber, G.M., Shepherd, B.S., Silverstein, J., Rexroad III, C.E. 2008. Effects of short term growth hormone treatment on the rainbow trout (Oncorhynchus mykiss) liver and muscle transcriptomes. Physiological Genomics. 32:380-9.
Molecular Characterization and Sex-Specific Tissue Expression of Estrogen Receptor Alpha (esr1), Estrogen Receptor Beta-a (esr2a) and Ovarian Aromatase (cyp19a1a) in Yellow Perch (Perca flavescens) - (Peer Reviewed Journal)
Lynn, S.G., Birge, W.J., Shepherd, B.S. 2008. Molecular Characterization and Sex-Specific Tissue Expression of Estrogen Receptor Alpha (esr1), Estrogen Receptor Beta-a (esr2a) and Ovarian Aromatase (cyp19a1a) in Yellow Perch (Perca flavescens). Comparative Biochemistry and Physiology, Part B. 149:126-147.
Development of Genetically-defined Yellow Perch (Perca flavescens) Broodstocks for Selective Breeding - (Abstract Only) - (30-Aug-07)
Developing Genetically Defined Yellow Perch (Perca flavescens) Broodstocks - (Abstract Only) - (18-Jul-07)
Developing Genetically Defined Yellow Perch (Perca flavescens) Broodstocks - (Abstract Only) - (18-Jul-07)
Supplementation of Taurine and Methionine to All-Plant Protein Diets for Rainbow Trout (Omcorhynchus Mykiss). Reprint Icon - (Peer Reviewed Journal)
Gaylord, T.G., Barrows, F., Teague, A.M., Johansen, K.A., Overturf, K.E., Shepherd, B.S. 2007. Supplementation of taurine and methionine to all-plant protein diets for rainbow trout (Omcorhynchus mykiss). Aquaculture 269:514-525.
Molecular characterization and sex-specific tissue expression of prolactin, somatolactin and insulin-like growth factor-I in yellow perch (Perca flavescens) - (Peer Reviewed Journal)
Lynn, S.G., Shepherd, B.S. 2007. Molecular characterization and sex-specific tissue expression of prolactin, somatolactin and insulin-like growth factor-I in yellow perch (Perca flavescens). Comparative Biochemistry and Physiology (Part B: Biochemistry and Molecular Biology). 147:412-427.
Endocrine and Orexigenic Effects of Growth Hormone Secretogogues in Rainbow Trout (Oncorhynchus Mykiss) - (Peer Reviewed Journal)
Shepherd, B.S., Johnson, J.K., Silverstein, J., Parhar, I., Vijayan, M.M., McGuire, A., Weber, G.M. 2006. Endocrine and orexigenic effects of growth hormone secretogogues in rainbow trout (Oncorhynchus mykiss). Comparative Biochemistry and Physiology. Part A 146(2007):390-399.
Improving Growth in Yellow Perch (Perca flavescens) - (Abstract Only) - (29-Aug-06)
Supplementation of Taurine and Methionine to All-Plant Protein Diets for Rainbow Trout (Oncorhynchus Mykiss). - (Abstract Only) - (06-Apr-06)
Effects of Recombinant Bovine Somatotropin (Rbst) on the Liver and Muscle Transcriptomes of High Growth Rate Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Gahr, S.A., Weber, G.M., Shepherd, B.S., Silverstein, J., Vallejo, R.L., Coulibaly, I., Rexroad III, C.E. 2005. Effects of Recombinant Bovine Somatotropin (rbst) on the Liver and Muscle Transcriptomes of High Growth Rate Rainbow Trout (Oncorhynchus Mykiss). Plant and Animal Genome Conference, Jan 2006. Abstract ID P534, pg 260.
Expression of Glucocorticoid Receptor in the Intestine of a Euryhaline Teleost, the Mozambique Tilapia (Oreochromis Mossambicus): Effect of Seawater Exposure and Cortisol Treatment - (Peer Reviewed Journal)
Takahashi, H., Sakamoto, T., Hyodo, S., Shepherd, B.S., Grau, E. 2006. Expression of glucocorticoid receptor in the intestine of a euryhaline teleost, the mozambique tilapia (Oreochromis mossambicus): effect of seawater exposure and cortisol treatment. Life Sciences 78(20): 2329-2335.
Effects of endocrine disrupters on the expression of growth hormone and prolactin mRNA in the rainbow trout pituitary. - (Peer Reviewed Journal)
Elango, A., Shepherd, B.S., Chen, T.T. 2006. Effects of endocrine disrupters on the expression of growth hormone and prolactin mRNA in the rainbow trout pituitary. General and Comparative Endocrinology. 145:116-127.
Purification, Cdna Cloning and Characterization of Ghrelin in Channel Catfish, Ictalurus Punctatus - (Peer Reviewed Journal)
Kaiya, H., Small, B.C., Bilodeau, A.L., Shepherd, B.S., Kojima, M., Hosoda, H., Kangawa, K. 2005. Purification, cdna cloning and characterization of ghrelin in channel catfish, ictalurus punctatus. General and Comparative Endocrinology 143:201-210.
Effects of Endocrine Disrupters on the Expression of Growth Hormone and Prolactin Mrna in the Rainbow Trout Pituitary - (Peer Reviewed Journal)
Elango, A., Shepherd, B.S., Chen, T.T. 2006. Effects of endocrine disrupters on the expression of growth hormone and prolactin mrna in the rainbow trout pituitary. General and Comparative Endocrinology. 145(2):116-127.
Salinity Acclimation Affects the Somatotropic Axis in Rainbow Trout - (Peer Reviewed Journal)
Shepherd, B.S., Drennon, K., Johnson, J., Nichols, J.W., Playle, R.C., Singer, T.D., Vijayan, M.M. 2004. Salinity acclimation affects the somatotropic axis in rainbow trout. American Journal of Physiology - Regulatory Integrative & Comparative Physiology, Vol. 288, P. R1385-1395
The Effects of Gradual Salinity Acclimation on the Somatotropic Axis in Rainbow Trout, Oncorhynchus Mykiss - (Abstract Only)
Shepherd, B.S., Drennon, K.D., Johnson, J.K., Nichols, J.W., Playle, R.C., Singer, T.D., Vijayan, M.M. 2005. The effects of gradual salinity acclimation on the somatotropic axis in rainbow trout, oncorhynchus mykiss. Aquaculture America Conference. On CD.
Analysis of Expressed Sequence Tags from the Liver and Brain of Estrogen Treated Yellow Perch (Perca flavescens) - (Abstract Only) - (31-Aug-04)
Endocrine and Orexigenic Effects of Growth Hormone Secretogogues in Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Shepherd, B.S., Johnson, J., Silverstein, J., Weber, G.M. 2004. Endocrine and orexigenic effects of growth hormone secretogogues in rainbow trout (oncorhynchus mykiss). International Symposium on Fish Endocrinology.
Control of Growth in Tilapia: Developments and Prospects - (Book / Chapter)
Shepherd, B.S., Weber, G.M., Vijayan, M., Seale, A., Riley, L., Rodriguez, M.F., Richman III, H., Hirano, T., Grau, G. 2006. Control of growth in tilapia: developments and prospects. Tilapias: Culture, Nutrition, and Feeding (3)73-137.
Growth Hormone Release: Interactions Between Ghrelin, Npy, Ghrh and Gnrh Neurons - (Abstract Only)
Soga, T., Kitahashi, T., Uchida, H., Sato, H., Shepherd, B.S., Parhar, I. 2004. Growth hormone release: interactions between ghrelin, npy, ghrh and gnrh neurons. International Symposium on Fish Endocrinology.
Cloning and Expression Studies for Multiple Endocrine Genes in Yellow Perch Perca Flavescens - (Abstract Only)
Lynn, S.G., Shepherd, B.S. 2004. Cloning and expression studies for multiple endocrine genes in yellow perch perca flavescens. World Aquaculture Society Meeting.
ARS News Articles
Last Modified: 10/25/2014
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