Alberto Nunez
Eastern Regional Research Center
Research Chemist
Phone: (215) 233-6639
Fax: (215) 233-6581
Room 0107 600 E MERMAID LN
ERRC WYNDMOOR, PA, 19038-8598
Publications
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Stone, C.L., Mcmahon, M.B., Fortis, L.L., Nunez, A., Smythers, G.W., Luster, D.G., Frederick, R.D. 2012. Gene expression and proteomic analysis of the formation of P. pachyrhizi appressoria. Biomed Central (BMC) Genomics. 13:269.
Qi, P.X., Nunez, A., Wickham, E.D. 2012. Reactions between beta-lactoglobulin and genipin: kinetics and characterization of the products. Journal of Agricultural and Food Chemistry. 60:4327-4335.
Gunther, N.W., Paul, M., Nunez, A., Liu, Y. 2012. pH fractionation and identification of proteins: comparing column isoelectric focusing vs liquid based focusing techniques. Journal of Separation Science. 35(12):1399-1406.
Piazza, G.J., Nunez, A., Garcia, R.A. 2012. Identification of highly active flocculant proteins in bovine blood. Applied Biochemistry and Biotechnology. 166(5):1203-1214.
Paul, M., Nunez, A., Van Hekken, D.L. 2012. The effect of milling on proteins in model Queso Fresco cheeses. Advances in Bioscience and Biotechnology. 3:1-6.
Yadav, M.P., Nunez, A., Hicks, K.B. 2011. Isolation, purification and identification of protein associated with corn fiber gum. Journal of Agricultural and Food Chemistry. 59:13289-13294.
Wyatt, V.T., Strahan, G.D., Nunez, A., Haas, M.J. 2011. Characterization of thermal and mechanical properties of oligo(glycerol-glutaric acid)s. Journal of Biobased Materials and Bioenergy. 5(1):92-101.
Gunther, N.W., Pang, H., Nunez, A., Uhlich, G.A. 2010. Comparative proteomics of E. coli O157:H7: two-dimensional gel electrophoresis vs. two-dimensional liquid chromatography separation. Open Proteomics Journal. 3:26-34.
Wyatt, V.T., Nunez, A., Strahan, G.D. 2010. The Lewis acid catalyzed synthesis of hyperbranched Oligo(glycerol-diacid)s in aprotic polar media. Journal of the American Oil Chemists' Society, 87(11):1539.
Cameron, R.G., Luzio, G.A., Savary, B.J., Nunez, A., Goodner, K. 2009. Digestion patterns of two commercial endopolygalacturonases on polygalacturonate oligomers with a degree of polymerization of 7 – 21. Proceedings of Florida State Horticultural Society. 122:295-302.
Zerkowski, J.A., Nunez, A., Strahan, G.D., Solaiman, D. 2009. Clickable Lipids: Azido and Alkynyl Fatty Acids and Triacylglycerols. Journal of the American Oil Chemists' Society. 86(11):1115-1121.
Wintermantel, W.M., Gulati Sakhuja, A.N., Larson, R.L., Hladky, L.L., Nunez, A., Hill, A.L. 2009. Proteome changes in sugarbeet in response to Beet necrotic yellow vein virus infection.Proc. Of the 35th biennial meeting of the American Society of Sugar Beet Technologists, Feb. 25-28, 2009, Orlando, FL: “Growing and Breeding,” Sections B&E Physiology, Biotechnology, Genetics and Germplasm. (Published CD)
Cardamone, J.M., Nunez, A., Garcia, R.A., Ramos, M. 2009. Characterizing wool keratin. Research Letters in Materials Science. 2009(5). Available: http://www.hindawi.com/journals/rlms/2009/147175.abs.html.
Gulati Sakhuja, A.N., Sears, J.L., Nunez, A., Liu, H. 2009. PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST PELARGONIUM ZONATE SPOT VIRUS COAT PROTEIN EXPRESSED IN ESCHERICHIA COLI AND APPLICATION FOR IMMUNODIAGNOSIS. Journal of Virological Methods. 160: 29-37.
Larson, R.L., Wintermantel, W.M., Hill, A.L., Fortis, L.L., Nunez, A. 2008. Proteome changes in sugar beet in response to Beet necrotic yellow vein virus. Physiological and Molecular Plant Pathology. doi:10.1016/j.pmpp.2008.04.003
Moreau, R.A., Doehlert, D.C., Welti, R., Isaac, G., Roth, M., Tamura, P., Nunez, A. 2008. The identification of mono-, di-, tri-, and tetragalactosyl-diacylglycerols and their natural estolides in oat kernels. Lipids. 43:533-548.
Zerkowski, J.A., Nunez, A., Solaiman, D. 2008. Structured Estolides: Control of Length and Sequence. Journal of the American Oil Chemists' Society. 83:277-284.
Moreau, R.A., Nunez, A., Hicks, K.B. 2007. HPLC, HPLC-MS and Lipidomics methods for the analysis of functional lipids in vegetable oils, nutraceuticals, and functional foods. Proceedings of 36th UJNR Food and Agricultural Panel, Tsukuba, Japan 2007. pp 177-180..
Chen, G., Schwartz, D.P., Nunez, A. 2007. Oxytetracycline Analysis in Honey Using a Portable Specific Analyzer. Proceedings of SPIE.6761:67610K-1-67610K-7
Nunez, A., Qi, P.X. 2007. Study of Intermediate Products from the Cross-linking Reactions of Genipin with Beta-Lactoglobulin and Related Peptides by MALDI-TOF/TOF Mass Spectrometry. Proceedings of the 55th ASMS Conference on Mass Spectrometry.
Larson, R.L., Hill, A.L., Nunez, A. 2007. Characterization of Protein Changes Associated with Sugar Beet (Beta vulgaris) Resistance and Susceptibility to Fusarium oxysporum.. Journal of Agricultural and Food Chemistry. 55:7905-7915.
Wyatt, V.T., Nunez, A., Foglia, T.A., Marmer, W.N. 2007. Optimizing the Acid Catalyzed Synthesis of Hyperbranched Poly(Glycerol-diacids) Oligomers [abstract]. Annual Meeting and Expo of the American Oil Chemists' Society. p. 100.
Ngo, H., Nunez, A., Lin, W., Foglia, T.A. 2007. Zeolite-Catalyzed Isomerization of Oleic Acid to Branched-Chain Isomers. European Journal of Lipid Science and Technology. 108:214-224.
Lee, J., Jones, K.C., Foglia, T.A., Nunez, A., Lee, J., Kim, N., Vu, P., Lee, K. 2007. Separation of Triacylglycerol Species from Interesterified Oils by High Performance Liquid Chromatography. Journal of the American Oil Chemists' Society. 84(3):211-217.
Wyatt, V.T., Nunez, A., Foglia, T.A., Marmer, W.N. 2006. Synthesis of hyperbranched poly(glycerol-diacid) oligomers. Journal of the American Oil Chemists' Society. 83(12):1033-1039.
Gunther, N.W., Hoan-Jen, P., Nunez, A., Uhlich, G.A. 2006. Comparing techniques for comparative proteomics: two-dimensional gel electrophoresis and two-dimensional liquid chromatography separation. Society of Industrial Microbiology. {Abstract}. P75.
Gunther, N.W., Nunez, A., Fortis, L.L., Solaiman, D. 2006. Proteomic based investigation of rhamnolipid production by Pseudomonas chlororaphis strain NRRL B-30761. Journal of Industrial Microbiology and Biotechnology 33:914-920.
Wyatt, V.T., Nunez, A., Foglia, T.A., Marmer, W.N. 2006. Acid catalyzed synthesis of hyperbranched poly(glycerol-diacid) oligomers [abstract]. Annual Meeting and Expo of the American Oil Chemists' Society. p. 72.
Richards, G.P., Nunez, A. 2006. Specificity of a vibrio vulnificus aminopeptidase toward kinins and other peptidyl substrates. Journal of Bacteriology. Mar. 2006. p. 2056-2062.
Wyatt, V.T., Nunez, A., Foglia, T.A., Marmer, W.N. 2006. Acid catalyzed synthesis of hyperbranched poly(glycerol-diacid) oligomers. Proceedings of American Chemical Society National Meeting. p. 46-47.
Cardamone, J.M., Nunez, A., Ashby, R.D., Dudley, R.L. 2006. Activated peroxide for enzymatic control of wool shrinkage part 1: elucidation. Textile Research Journal. 76(2):99-108.
Ashby, R.D., Nunez, A., Solaiman, D., Foglia, T.A. 2005. Sophorolipid biosynthesis from the biodiesel co-product stream. Journal of the American Oil Chemists' Society. 82(9):625-630.
Savary, B.J., Nunez, A., Cameron, R.G. 2005. Biochemical and chemical diversity of pectin methylesterases: specific isoform identification by mass spectrometry. Abstract. Pacifichem 2005, International Chemical Congress of Pacific Basin. Pectin Chemistry #221.
Nunez, A., Bayles, D.O., Fortis, L.L. 2005. Improved technique for robotic spotting and detection of low abundance proteins by maldi-tof/tof mass spectrometry. Meeting Abstract.
Nunez, A., Moreau, R.A., Foglia, T.A. Liquid chromatography/mass spectrometry for the analysis of biosurfant glycolipids secreted by microorganisms. Book Chapter. W. C. Byrdwell (ed) in Modern Methods for Lipids Analysis by Liquid Chromatography/Mass Spectrometry and Related Techniques, pp.447-471, 2005.
Savary, B.J., Nunez, A., Cameron, R.G. 2005. Identification of plant pectin methylesterase isoforms by mass spectromety peptide profiling. Meeting Abstract. Annual Meeting of American Society of Plant Biologists. Paper #999.
Solaiman, D., Ashby, R.D., Zerkowski, J.A., Nunez, A., Foglia, T.A. 2005. Production and modification of sophorolipid biosurfactant [abstract]. Annual Meeting and Expo of the American Oil Chemists' Society. p. 17.
Savary, B.J., Nunez, A., Cameron, R.G. Mass spectroscopy profiling of tryptic-peptides for identifying pectin methylesterases isolated from valencia orange [(citrus sinensis (l.) osbeck] fruit. Abstract 229TH NATIONAL MEETING, AMERICAN CHEMICAL SOCIETY, SAN DIEGO, CA., March 13-17, 2005. Cell 118.
Gunther, N.W., Nunez, A., Fett, W.F., Solaiman, D. 2005. Production of rhamnolipids by pseudomonas chlororaphis, a non-pathogenic bacterium. Applied and Environmental Microbiology. 71(5):2288-2293.
Ashby, R.D., Nunez, A., Solaiman, D., Foglia, T.A. 2004. Lipidic precursors to control the synthesis and properties of microbial sophorolipids and polyhydroxyalkanoates. Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel Annual Meeting. p. 225:229.
Ashby, R.D., Nunez, A., Solaiman, D., Foglia, T.A. 2004. Production and structural variability of sophorolipids derived from the fermentation of renewable resources [abstract]. American Oil Chemists' Society Meeting. p. 29.
Savary, B.J., Nunez, A. 2004. Gas chromatography-mass spectrometry determination of ester contents following base- and enzyme-hydrolysis of cell wall polysaccharides. (Abstract). 228th American Chemical Society National Meeting, Philadelphia, PA. August 22-26, 2004. CARB:61.
Muhitch, M.J., Savary, B.J., Nunez, A., Wicklow, D.T., Kendra, D.F., Alessandro, R.T., Shatters, R.G. 2004. Identification of citrus leaf proteins that inhibit the growth of the maize pathogen Fusarium verticillioides in vitro. Plant Biology.
Savary, B.J., Nunez, A. 2004. Application of a new gc-ms method for determining ester contents following alkaline or enzymatic hydrolysis of sugar beet pulp and pectin. Meeting Abstract in Proceedings of the Sugar Processing Research Institute Conference, p.449-453.
Solaiman, D., Ashby, R.D., Nunez, A., Foglia, T.A. 2004. Production of sophorolipids using soy molasses as substrate. Biotechnology Letters. 26:1241-1245.
Foglia, T.A., Jones, K.C., Nunez, A., Phillips, J.G., Mittelbach, M. 2004. Comparison of chromatographic methods for the determination of bound glycerol in biodiesel. Chromatographia. 60:305-311.
Nunez, A., Ashby, R.D., Foglia, T.A., Solaiman, D. 2004. LC/MS analysis and lipase modification of the sophorolipids produced by rhodotorula bogoriensis. Biotechnology Letters. 26:1087-1093.
Nunez, A., Ashby, R.D., Foglia, T.A., Solaiman, D. Characterization of sophorolipids produced by rhodotorula bogoriensis and their lipase-mediated rearrangement products. In: Proceedings of the ASMS Conference on Mass Spectrometry and Allied Topics. May 22-27, 2004, Nashville, Tennessee. 2004 CDROM.
Savary, B.J., Newswanger, B., Nunez, A. 2004. Application of a new gc-ms method for determining ester contents following base- and enzyme-hydrolysis of sugar beet pulp and pectin. Meeting Abstract for Sugar Processing Research Institute Conference, Atlanta, GA., April 4-7, 2004.
Ashby, R.D., Solaiman, D., Nunez, A., Foglia, T.A. 2004. Tricylglycerol-based production of environmentally benign, value-added polymers and surfactants [abstract]. Annual Meeting of the American Oil Chemists' Society. p. 32.
Nunez, A., Ashby, R.D., Foglia, T.A., Solaiman, D. 2004. Characterization of sophorolipids produced by rhodotorula bogoriensis and their lipase-mediated rearrangement products. American Society for Mass Spectrometry. Paper No. ThPI 151.
Solaiman, D., Ashby, R.D., Nunez, A., Foglia, T.A. 2004. Fermentative production of biosurfactants from agricultural lipids and byproducts [abstract]. Annual Meeting of the American Oil Chemists' Society. p. 156.
Piazza, G.J., Nunez, A., Foglia, T.A. 2004. Fatty epoxide synthesis with peroxygenase and hydrolysis with alumina and perchloric acid [abstract]. 95th Annual Meeting of the American Oil Chemists' Society. p. 28.
Savary, B.J., Nunez, A., Liu, L.S., Yoo, S. 2003. Pectin acetylesterase - analysis and application for sugar beet pectin utilization. Proceedings of the 1st Joint International Beet Research - American Society of Sugar Beet Technologists Congress, pp. 917-920. Beet Sugar Development Foundation, Denver, CO.
Ashby, R.D., Nunez, A., Solaiman, D., Foglia, T.A. 2003. Synthesis of biopolymers and biosurfactants from glycerol and glycerol-based waste streams. In: Proceedings of United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel, November 9-15, 2003, Tsukuba, Japan. p. 269-278.
Modern mass spectrometry - Four types of user-friendly instruments and their applications for lipid chemistry and biochemistry.Inform 14(9)p.536-539.2003
Cardamone, J.M., Yao, J., Nunez, A. 2004. Dcca shrinkproofing of wool - part i: the importance of antichlorination. Textile Research Journal. 74(6):555-560.
Savary, B.J. and Nunez, A. 2003. Gas chromatography-mass spectrometry quantification of the methanol and acetic acid contents of pectin using headspace microextraction with stable isotope dilution. Journal of Chromatography A. 1017: p.151-159.
NUNEZ, A., FOGLIA, T.A., ASHBY, R.D. ENZYMATIC SYNTHESIS OF A GALACTOPYRANOSE SOPHOROLIPID FATTY ACID-ESTER. BIOTECHNOLOGY LETTERS. v. 25. p. 1291-1297.
Piazza, G.J., Nunez, A., Foglia, T.A. 2003. Hydrolysis of mono- and diepoxyoctadecanoates by alumina. Journal of the American Oil Chemists' Society. 80(9):901-904.
PIAZZA, G.J., NUNEZ, A., FOGLIA, T.A. ISOLATION OF UNSATURATED DIOLS AFTER OXIDATION OF CONJUGATED LINOLEIC ACID WITH PEROXYGENASE. LIPIDS. 2003. VOL. 38, no. 3. p. 255-261.
Savary, B.J., Nunez, A., Yoo, S., Liu, L.S. 2003. Pectin acetylesterase - analysis and application for sugar beet pectin utilization. Abstract for 1st Joint International Institute for Beet Research And American Society for Sugar Beet Technologists Congress. p.68. San Antonio, TX, 2003.
PIAZZA, G.J., NUNEZ, A., FOGLIA, T.A. EPOXIDATION OF FATTY ACIDS, FATTY METHYL ESTERS, AND ALKENES BY IMMOBILIZED OAT SEED PEROXYGENASE. JOURNAL MOLECULAR CATALYSIS B: ENZYMATIC. 2003. v. 21. p. 143-151.
HAAS, M.J., MICHALSKI, P.J., RUNYON, S., SCOTT, K.M., NUNEZ, A. PRODUCTION OF FAME FROM ACID OIL, A BY-PRODUCT OF VEGETABLE OIL REFINING. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY. 2003. V. 80. p. 97-102.
Solaiman, D., Ashby, R.D., Foglia, T.A., Nunez, A., Marmer, W.N. 2003. Fermentation-based processes for the conversion of fats, oils and derivatives into biopolymers and biosurfactants. In: Proceedings of the United States-Japan Cooperative Program in Natural Resources (UJNR) Protein Resources Panel, November 9-11, 2003, Tsukuba, Japan. p. V1-V10.
SINGH, S.K., FELSE, P.A., GROSS, R.A., NUNEZ, A., FOGLIA, T.A. REGIOSELECTIVE ENZYME-CATALYZED SYNTHESIS OF SOPHOROLIPID ESTERS, AMIDES, AND MULTIFUNCTIONAL MONOMERS. JOURNAL OF ORGANIC CHEMISTRY. 2003. V. 68(14). p. 5466-5477.
NUNEZ, A., FOGLIA, T.A., PIAZZA, G.J. PURIFICATION OF LIPOXYGENASE FROM CHLORELLA: PRODUCTION OF THE 9 AND 13-HYDROPEROXIDE DERIVATES OF LINOLEIC ACID. LIPIDS. 2002. v. 37. p. 1027-1032.