Projects

TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES Appropriated (D)   Accession Number: 421870

SUSCEPTIBILITY OF CATTLE WITH THE E211K PRNP ALLELE TO BSE Specific Cooperative Agreement (S)   Accession Number: 413249

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Relationships between PrPSc stability and incubation time for United States scrapie strains in a natural host system - (Peer Reviewed Journal)

Vrentas, C.E., Greenlee, J.J., Tatum, T.L., Nicholson, E.M. 2012. Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system. PLoS ONE. 7(8):e43060.

Susceptibility of cattle to the agent of chronic wasting disease from elk after intracranial inoculation - (Peer Reviewed Journal)

Greenlee, J.J., Nicholson, E.M., Smith, J.D., Kunkle, R.A., Hamir, A.N. 2012. Susceptibility of cattle to the agent of chronic wasting disease from elk after intracranial inoculation. Journal of Veterinary Diagnostic Investigation. 24(6):1087-1093.

Stability of PrP**Sc from cattle inoculated with different BSE strains - (Abstract Only)

Nicholson, E.M., Vrentas, C.E., Greenlee, J.J. 2012. Stability of PrPSc from cattle inoculated with different BSE strains. 26th Annual Symposium of The Protein Society. p. 210.

Use of an ELISA-based stability assay to examine host genotype, PrP**Sc stability, and incubation time relationships in U.S. livestock prion strains - (Abstract Only)

Vrentas, C.E., Nicholson, E.M., Greenlee, J.J. 2012. Use of an ELISA stability assay to examine host genotype, PrPSc stability, and incubation time relationships in U.S. livestock prion strains. 26th Annual Symposium of The Protein Society. p. 211.

A comparative analysis of rapid methods for purification and refolding of recombinant bovine prion protein - (Peer Reviewed Journal)

Vrentas, C.E., Onstot, S., Nicholson, E.M. 2012. A comparative analysis of rapid methods for purification and refolding of recombinant bovine prion protein. Protein Expression and Purification. 82(2):380-388.

Prolonged incubation time in sheep with prion protein containing lysine at position 171 - (Peer Reviewed Journal)

Greenlee, J.J., Zhang, Xia, Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2012. Prolonged incubation time in sheep with prion protein containing lysine at position 171. Journal of Veterinary Diagnostic Investigation. 24(3):554-558.

Experimental Interspecies Transmission Studies of the Transmissible Spongiform Encephalopathies to Cattle: Comparison to Bovine Spongiform Encephalopathy in Cattle - (Review Article)

Hamir, A.N., Kehrli, Jr., M.E., Kunkle, R.A., Greenlee, J.J., Nicholson, E.M., Richt, J.A., Miller, J.M., Cutlip, R.C. 2011. Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle. Journal of Veterinary Diagnostic Investigation. 23(3):407-420.

PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA - (Peer Reviewed Journal)

Nicholson, E.M., Greenlee, J.J., Hamir, A.N. 2011. PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA. BMC Research Notes. 4(1):432.

Enrichment of PrPSc in Formalin Fixed Paraffin Embedded Tissues Prior to Analysis by Western Blot - (Peer Reviewed Journal)

Nicholson, E.M. 2011. Enrichment of PrPSc in formalin-fixed, paraffin-embedded tissues prior to analysis by Western blot. Journal of Veterinary Diagnostic Investigation. 23(4):790-792.

Formalin Fixed Paraffin Embedded Tissue as a Starting Point for PrPSc Detection by ELISA - (Abstract Only)

Nicholson, E.M. 2010. Formalin fixed paraffin embedded tissue as a starting point for PrPSc detection by ELISA [abstract]. Prion. 4(3):213.

Experimental Transmission of Chronic Wasting Disease (CWD) from Elk (Cervus elaphus nelsoni) and White-tailed Deer (Odocoileus virginianus) to Fallow Deer (Dama dama) by Intracerebral Route: Final Report - (Peer Reviewed Journal)

Hamir, A.N., Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Richt, J.A., Miller, J.M., Hall, M. 2011. Experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer by intracerebral route: final report. Canadian Journal of Veterinary Research. 75(2):152-156.

Manganese Upregulates Cellular Prion Protein and Contributes to Altered Stabilization and Proteolysis: Relevance to Role of Metals in Pathogenesis of Prion Disease - (Peer Reviewed Journal)

Choi, C.J., Anantharam, V., Martin, D.P., Nicholson, E.M., Richt, J., Kanthasamy, A., Kanthasamy, A. 2010. Manganese Upregulates Cellular Prion Protein and Contributes to Altered Stabilization and Proteolysis: Relevance to Role of Metals in Pathogenesis of Prion Disease. Toxicological Sciences. 115(2):535-546.

Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-affected Sheep with the AA136, QR171 Prion Protein Genotype - (Abstract Only)

Kunkle, R.A., Hamir, A.N., Richt, J., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2009. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-affected Sheep with the AA136, QR171 Prion Protein Genotype [abstract]. American Association of Veterinary Laboratory Diagnosticians. Paper No. 186.

Susceptibility of Cattle to First-Passage Intracerebral Inoculation with Chronic Wasting Disease Agent from Elk - (Abstract Only)

Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Hamir, A.N. Susceptibility of Cattle to First-Passage Intracerebral Inoculation with Chronic Wasting Disease Agent from Elk [abstract]. American College of Veterinary Pathologists Annual Meeting. Veterinary Pathology. 46(5):1058.

Guanidinium Chloride Unfolding of PrP**Sc Exhibits Marked Differences Between Genotypes - (Abstract Only)

Nicholson, E.M., Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2009. Guanidinium Chloride Unfolding of PrP**Sc Exhibits Marked Differences Between Genotypes [abstract]. Prion 2009. p. 182.

Prolonged Incubation Time in Sheep with QK171 Genotype - (Abstract Only)

Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2009. Prolonged Incubation Time in Sheep with QK171 Genotype [abstract]. Prion 2009. p. 120.

Origins of BSE: evidence of sporadic and heritable BSE - (Abstract Only)

Nicholson, E.M., Brunelle, B.W., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Origins of BSE: Evidence of Sporadic and Heritable BSE [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 7.

Frequencies of polymorphisms associated with BSE resistance differ significantly between Bos taurus, Bos indicus, and composite cattle - (Peer Reviewed Journal)

Brunelle, B.W., Greenlee, J.J., Seabury, C.M., Brown II, C.E., Nicholson, E.M. 2008. Frequencies of Polymorphisms Associated with BSE Resistance Differ Significantly Between Bos taurus, Bos indicus, and Composite Cattle. BioMed Central (BMC) Veterinary Research. 4(1):36. Available: http://www.biomedcentral.com/1746-6148/4/36.

Strain typing of U.S. scrapie strains using a panel of inbred mice - (Abstract Only)

Greenlee, J.J., Kunkle, R.A., Nicholson, E.M., Hamir, A.N., Bulgin, M.S., Richt, J. 2009. Strain Typing of U.S. Scrapie Strains Using a Panel of Inbred Mice [abstract]. American College of Veterinary Pathologists. Paper No. 121. p. 762.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Abstract Only)

Hamir, A.N., Cutlip, R.C., Miler, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and Their Differentiation from Bovine Spongiform Encephalopathy [abstract]. American College of Veterinary Pathologists. Paper No. 153. p. 770.

Identification of a heritable polymorphism in bovine PRNP associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE - (Peer Reviewed Journal)

Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in Bovine PRNP Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE. PloS ONE. 3(8):e2912.

Identification of a heritable polymorphism in the bovine prion gene associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE - (Abstract Only)

Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in the Bovine Prion Gene Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE [abstract]. Prion 2008. p. 114.

Divalent metals stabilize cellular prion proteins and alter the rate of proteinase-K dependent limited proteolysis - (Abstract Only)

Kanthasamy, A.G., Choi, C., Nicholson, E.M., Anantharam, V., Richt, J.A., Kanthasamy, A. 2008. Divalent Metals Stabilize Cellular Prion Proteins and Alter the Rate of Proteinase-K Dependent Limited Proteolysis [abstract]. Prion 2008. p. 104.

Lack of prion accumulation in lymphoid tissues of scrapie-affected sheep with AA136, QR171 polymorphism type - (Abstract Only)

Kunkle, R.A., Hamir, A.N., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-Affected Sheep with AA136, QR171 Polymorphism Type [abtract]. Prion 2008. p. 75.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Abstract Only)

Hamir, A.N., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and their Differentiation from Bovine Spongiform Encephalopathy [abstract]. Prion 2008. p. 65.

Western-blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep - (Peer Reviewed Journal)

Kunkle, R.A., Nicholson, E.M., Lebepe-Mazur, S., Orcutt, D.L., Srinivas, M.L., Greenlee, J.J., Alt, D.P., Hamir, A.N. 2008. Western blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep. Journal of Veterinary Diagnostic Investigation. 20(4):522-526.

Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route - (Peer Reviewed Journal)

Hamir, A.N., Richt, J., Miller, J.M., Kunkle, R.A., Hall, S., Nicholson, E.M., Orourke, K.I., Greenlee, J.J., Williams, E.S. 2008. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route. Veterinary Pathology. 45(3):297-306.

Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer - (Peer Reviewed Journal)

Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Miller, J.M., Hall, S.M., Schonenbrucher, H., Brunelle, B.W., Richt, J.A. 2008. Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer. Journal of Comparative Pathology. 138(2-3):121-130.

Allele, genotype, and haplotype data for BSE-resistance polymorphisms from healthy U.S. holstein cattle - (Peer Reviewed Journal)

Brunelle, B.W., Kehrli, Jr., M.E., Stabel, J.R., Moody Spurlock, D., Hansen, L.B., Nicholson, E.M. 2007. Allele, genotype, and haplotype data for bovine spongiform encephalopathy-resistance polymorphisms from healthy US holstein cattle. Journal of Dairy Science. 91:338–342.

Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer (Dama dama) - (Abstract Only)

Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Miller, J.M., Hall, S.M., Schonenbrucher, H., Brunelle, B.W., Richt, J.A. 2007. Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer (Dama dama) [abstract]. European Society of Veterinary Pathology 25th Annual Meeting. Paper No. 49. p. 104.

Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy - (Proceedings)

Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2007. Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians 13th International Symposium, November 11-14, 2007, Melbourne, Australia. p. 29.

Experimental chronic wasting disease (CWD) in fallow deer - (Abstract Only)

Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Miller, J.M., Hall, S.M., Schonenbrucher, H.H., Richt, J.A. 2007. Preliminary observations on the experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deet to fallow deer [abstract]. Prion 2007. Paper No. PO2.43.

Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities - (Government Publication)

Kehrli, Jr., M.E., O'Rourke, K.I., Hamir, A.N., Richt, J.A., Nicholson, E.M., Silva, C.J., Edelman, D., Gay, C.G. 2007. Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities [government white paper]. Beltsville, MD: Interagency Working Group on Prion Science, Subcommittee on Pathobiology and Diagnostics. USDA, Agriculture Research Service. 33 p.

Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot - (Peer Reviewed Journal)

Nicholson, E.M., Kunkle, R.A., Hamir, A.N., Lebepe Mazur, S., Orcutt, D. 2007. Detection of the disease-associated isoform of the prion protein in formalin-fixed tissues by Western blot. Journal of Veterinary Diagnostic Investigation. 19:548-552.

Cross-species transmission of TSE - (Proceedings)

Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J. 2007. Cross-species transmission of TSE. In: Proceedings of the American College of Veterinary Internal Medicine. 2007 ACVIM Forum, June 6-9, 2007, Seattle, Washington. Paper No. 160.

EXPOSURE OF SHEEP SCRAPIE BRAIN HOMOGENATE TO RUMEN-SIMULATING CONDITIONS DOES NOT RESULT IN A REDUCTION OF PRP**SC LEVELS - (Peer Reviewed Journal)

Nicholson, E.M., Richt, J.A., Rasmussen, M.A., Hamir, A.N., Lebepe-Mazur, S, Horst, R.L. 2007. Exposure of sheep scrapie brain homogenate to rumen-simulating conditions does not result in a reduction of PrP**sc levels. Letters in Applied Microbiology. 44:631-636.

Detection of PrP**Sc in formalin-fixed tissues by western blot - (Abstract Only)

Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2006. Detection of PrP**Sc in formalin-fixed tissues by western blot [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 16.

Identification and characterization of two bovine spongiform encephalopathy cases diagnosed in the United States - (Peer Reviewed Journal)

Richt, J.A., Kunkle, R.A., Alt, D., Nicholson, E.M., Hamir, A.N., Czub, S., Kluge, J., Davis, A.J., Hall, S.M. 2007. Identification and characterization of two bovine spongiform encephalopathy cases diagnosed in the United States. Journal of Veterinary Diagnostic Investigation. 19(2):142-154.

MANGANESE UPREGULATES CELLULAR PRION PROTEINS AND INHIBITS THE RATE OF PROTEINASE-K DEPENDENT LIMITED PROTEOLYSIS IN NEURONAL CELLS - (Abstract Only)

Choi, C.J., Anantharam, V., Nicholson, E., Richt, J., Kanthasamy, A., Kanthasamy, A.G. 2006. Manganese upregulates cellular prion proteins and inhibits the rate of proteinase-K dependent limited proteolysis in neuronal cells [abstract]. Society for Neuroscience. Paper No. 74.23.

DETECTION OF PRP**SC IN FORMALIN-FIXED TISSUES BY WESTERN BLOT - (Abstract Only)

Nicholson, E.M., Hamir, A.N., Kunkle, R.A. 2006. Detection of PrP**sc in formalin-fixed tissues by Western blot [abstract]. United States Animal Health Association. p. 332.

ATTEMPTED TRANSMISSION OF SCRAPIE AND CWD TO SWINE: PRELIMINARY FINDINGS - (Proceedings)

Greenlee, J.J., Kunkle, R.A. Nicholson, E.M., Lager, K.M., Hamir, A.N. 2006. Attempted transmission of scrapie and CWD to swine: preliminary findings. 19th International Pig Veterinary Society Congress. p. 386.

TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES: DETECTION & DIAGNOSIS - (Book / Chapter)

Nicholson, E.M., Richt, J.A. 2003. Transmissible spongiform encephalopathies: detection & diagnosis. Encyclopedia of Animal Science [online]. 1(1). http://www.dekker.com/sdek/abstract~db=enc~content=a713617959.

TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES: BIOLOGY AND DISEASE - (Book / Chapter)

Nicholson, E.M., Richt, J.A. 2006. Transmissible spongiform encephalopathies: biology and disease. Encyclopedia of Animal Science [online]. 1(1). Available: http://www.dekker.com/sdek/abstract~db=enc~content=a713617944.

NATURAL AND EXPERIMENTAL PRION DISEASES OF CATTLE - (Abstract Only)

Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J., Miller, J.M., Cutlip, R., Davis, A.J., Hall, S.M. 2005. Natural and experimental prion diseases of cattle [abstract]. Third International Rushmore Conference on Enteric Diseases. Paper No. 14.

BIOCHEMISTRY OF PRION DISEASES - (Abstract Only)

Nicholson, E.M., Greenlee, J.J., Richt, J., Hamir, A.N., Kunkle, R.A., Kehrli, Jr., M.E. 2005. Biochemistry of prion diseases. Association of Analytical Communities International, Midwest Section [abstract]. Abstract No. 601. p. 38.

LOSS OF PRION PROTEIN IMMUNOREACTIVITY AFTER BOILING IN SATURATED CALCIUM HYDROXIDE SOLUTION - (Abstract Only)

Greenlee, J.J., Nicholson, E.M., Hamir, A.N., Richt, J.A., Kunkle, R.A., Noyes, G., Hotzapple, M.T., Kehrli Jr., M.E. 2005. Loss of prion protein immunoreactivity after boiling in saturated calcium hydroxide solution [abstract]. Keystone Symposia: Molecular Mechanisms of Transmissible Spongiform Encephalopathies. Poster No. 125.

ESTABLISHMENT OF A REAL TIME-PCR BASED ASSAY FOR THE DETERMINATION OF OVINE PRP MRNA - (Abstract Only)

Nicholson, E.M., Young, A.J., Richt, J. 2004. Establishment of a real time-PCR based assay for the determination of ovine PrP mRNA [abstract]. Animal Prion Diseases and the Americas. p. 90.