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United States Department of Agriculture

Agricultural Research Service

Related Topics

Gaylen A Uhlich
Molecular Characterization of Foodborne Pathogens
Research Microbiologist

Phone: (215) 233-6740
Fax: (215) 233-6581
Room 2112.2
600 E MERMAID LN
ERRC
WYNDMOOR, PA, 19038-8598

Projects
MICROBIAL COMMUNITIES AND INTERACTIONS AND THEIR IMPACT ON FOOD SAFETY
Appropriated (D)
  Accession Number: 420978

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Growth media and temperature effects on biofilm formation by serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli - (Peer Reviewed Journal)
Uhlich, G.A., Chen, C., Cottrell, B.J., Nguyen, L.T. 2014. Growth media and temperature effects on biofilm formation by serotype O157:H7 and non-O157 Shiga toxin-producing Escherichia coli. FEMS Microbiology Letters. 354:133-141.
Genetically marked strains of Shiga toxin-producing E. coli O157:H7 and non-O157 for detection and modeling - (Abstract Only) - (25-Mar-14)
Production and regulation of functional amyloid curli fimbriae by Shiga toxin-producing Escherichia coli - (Abstract Only) - (21-Mar-14)
Media composition and incubation temperature affect Congo red dye affinity of Shiga toxin-producing Escherichia coli - (Abstract Only)
Uhlich, G.A., Chen, C., Cottrell, B.J., Nguyen, L.T. 2014. Media composition and incubation temperature affect Congo red dye affinity of Shiga toxin-producing Escherichia coli. Meeting Abstract. MA.
Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains - (Peer Reviewed Journal)
Chen, C., Hofmann, C.S., Cottrell, B.J., Strobaugh Jr, T.P., Paoli, G., Nguyen, L.T., Yan, X., Uhlich, G.A. 2013. Phenotypic and genotypic characterization of biofilm forming capability in non-O157 Shiga toxin-producing Escherichia coli strains. PLoS One. 8(12):e84863.
Non-labeled quantitative proteomic comparison identifies differences in acid resistance between Escherichia coli O157:H7 curli production variants Reprint Icon - (Peer Reviewed Journal)
Gunther, N.W., Liu, Y., Nunez, A., Paul, M., Uhlich, G.A. 2014. Non-labeled quantitative proteomic comparison identifies differences in acid resistance between Escherichia coli O157:H7 curli production variants. Foodborne Pathogens and Disease. 11:30-37.
Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157:H7 - (Peer Reviewed Journal)
Uhlich, G.A., Chen, C., Cottrell, B.J., Hofmann, C.S., Dudley, E., Strobaugh Jr, T.P., Nguyen, L.T. 2013. Phage insertion in mlrA and variations in rpoS limit curli expression and biofilm formation in Escherichia coli serotype O157:H7. Microbiology. 159:1586-1596.
Prophage insertions in mlrA are not the major obstacle to biofilm formation in Escherichia coli O157:H7 - (Abstract Only)
Uhlich, G.A., Chen, C., Dudley, E.G., Hofmann, C.S., Cottrell, B.J. 2014. Prophage insertions in mlrA are not the major obstacle to biofilm formation in Escherichia coli O157:H7. Meeting Abstract. MA.
Mutations in the rpoS gene are the major limiting factor for biofilm formation in Escherichia coli serotype O157:H7 clinical isolates - (Abstract Only)
Chen, C., Uhlich, G.A., Strobaugh Jr, T.P., Cottrell, B.J., Dudley, E.G., Hofmann, C.S., Nguyen, L.T. 2013. Mutations in the rpoS gene are the major limiting factor for biofilm formation in Escherichia coli serotype O157:H7 clinical isolates. Meeting Abstract. MA.
Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms - (Peer Reviewed Journal)
Uhlich, G.A., Chen, C., Cottrell, B.J., Irwin, P.L., Phillips, J.G. 2012. Peroxide resistance in Escherichia coli serotype O157:H7 biofilms is regulated by both RpoS dependent and independent mechanisms. Microbiology. 158:2225-2234.
Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms - (Abstract Only)
Uhlich, G.A., Chen, C., Irwin, P.L. 2012. Understanding the role of the catalase/peroxide genes in H2O2 resistance of E. coli serotype O157:H7 biofilms [abstract]. 1:1.
Regulation of the csgD promoter by global regulators HN-S, IHF, and RpoS in E. coli O157:H7 isolates - (Abstract Only)
Chen, C., Uhlich, G.A. 2012. Regulation of the csgD promoter by global regulators HN-S, IHF, and RpoS in E. coli O157:H7 isolates [abstract]. Poster p-3-121.
A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's - (Peer Reviewed Journal)
Irwin, P.L., Nguyen, L.T., Chen, C., Uhlich, G.A., Paoli, G. 2012. A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's. Analytical and Bioanalytical Chemistry. 402:2713-2725.
A cloning vector for creation of Escherichia coli lacZ translational fusions and generation of linear template for chromosomal integrations - (Peer Reviewed Journal)
Uhlich, G.A., Chen, C. 2012. A cloning vector for creation of Escherichia coli lacZ translational fusions and generation of linear template for chromosomal integrations. Plasmid Journal. 67(3):259-263.
Sequence analysis of a group of low molecular-weight plasmids carrying multiple IS903 elements flanking a kanamycin resistance aph gene in Salmonella enterica serovars - (Peer Reviewed Journal)
Chen, C., Strobaugh Jr, T.P., Lindsey, R.L., Frye, J.G., Uhlich, G.A. 2011. Sequence analysis of a group of low molecular-weight plasmids carrying multiple IS903 elements flanking a kanamycin resistance aph gene in Salmonella enterica serovars. Plasmid Journal. 65:246-252.
Comparative proteomics of E. coli O157:H7: two-dimensional gel electrophoresis vs. two-dimensional liquid chromatography separation - (Peer Reviewed Journal)
Gunther, N.W., Pang, H., Nunez, A., Uhlich, G.A. 2010. Comparative proteomics of E. coli O157:H7: two-dimensional gel electrophoresis vs. two-dimensional liquid chromatography separation. Open Proteomics Journal. 3:26-34.
Esherichia coli serotype O157:H7 retention on solid surfaces and peroxide resistance is enhanced by dual-strain biofilm formation - (Peer Reviewed Journal)
Uhlich, G.A., Rogers, D., Mosier, D.A. 2010. Esherichia coli serotype O157:H7 retention on solid surfaces and peroxide resistance is enhanced by dual-strain biofilm formation. Foodborne Pathogens and Disease. 7(8):935-943.
KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157:H7 - (Peer Reviewed Journal)
Uhlich, G.A. 2009. KatP contributes to OxyR-regulated hydrogen peroxide resistance in Escherichia coli serotype O157:H7. Microbiology. 155(11):3589-3598.
Molecular mechanisms involved in biofilm formation by food-associated bacteria - (Book / Chapter)
Smith, J.L., Fratamico, P.M., Uhlich, G.A. 2009. Molecular mechanisms involved in biofilm formation by food-associated bacteria. Book Chapter. In: Fratamico, P.M., Annous, B.A., Gunther, N.W., editors. Biofilms in the Food and Beverage Industries. Oxford, UK: Woodhead Publishing, Ltd. p. 42-98.
The CsgA and Lpp proteins affect HEp-2 cell invasion, motility, and biofilm formation in a strain of Escherichia coli O157:H7 - (Peer Reviewed Journal)
Uhlich, G.A., Gunther, N.W., Bayles, D.O., Mosier, D.A. 2009. The CsgA and Lpp proteins affect HEp-2 cell invasion, motility, and biofilm formation in a strain of Escherichia coli O157:H7. Infection and Immunity. 77(4):1543-1552.
Investigation of E. coli O157:H7 Mixed-Species Biofilm Formation - (Proceedings)
Uhlich, G.A. 2008. Investigation of E. coli O157:H7 Mixed-Species Biofilm Formation. Meeting Abstract.
The CsgA and Lpp proteins affect motility and cell invasion in a strain of Escherichia coli O157:H7 - (Abstract Only)
Uhlich, G.A., Bayles, D.O., Mosier, D.A. The CsgA and Lpp proteins affect motility and cell invasion in a strain of Escherichia coli O157:H7. Meeting Abstract.
Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006 - (Peer Reviewed Journal)
Uhlich, G.A., Sinclair, J.R., Warren, N.G., Chmielecki, W.A., Fratamico, P.M. 2008. Characterization of Shiga toxin-producing Escherichia coli associated with two multi-state foodborne outbreaks in 2006. Applied and Environmental Microbiology. 74:1268-1272.
Characterization of Shiga toxin-producing Escherichia coli isolated from different sources in 2006 - (Abstract Only)
Uhlich, G.A. 2007. Characterization of Shiga toxin-producing Escherichia coli isolated from different sources in 2006. Meeting Abstract.
Characterization of Shiga toxin-producing Escherichia coli isolated from different sources - (Abstract Only)
Uhlich, G.A. 2007. Characterization of Shiga toxin-producing Escherichia coli isolated from different sources. Meeting Abstract . Meeting Abstract.
The Lpp lipoprotein suppresses motility in a biofilm-forming strain of Escherichia coli O157:H7 - (Abstract Only)
Uhlich, G.A., Bayles, D.O. 2007. The Lpp lipoprotein suppresses motility in a biofilm-forming strain of Escherichia coli O157:H7. International Association of Food Protection. Abstract. P5-15.
ANALYSIS OF THE "RDAR" PHENOTYPE OF A STRAIN OF ESCHERICHIA COLI O157:H7 AND ITS ROLE IN BIOFILM FORMATION AND RESISTANCE TO ANTIBACTERIAL AGENTS - (Peer Reviewed Journal)
Uhlich, G.A., Cooke, P.H., Solomon, E.B. 2006. Analysis of the "rdar" phenotype of a strain of escherichia coli o157:h7 and its role in biofilm formation and resistance to antibacterial agents. Applied and Environmental Microbiology. Vol. 72:2564-2572.
Analysis of biofilm formation by Escherichia coli serotype O157:H7 - (Abstract Only)
Uhlich, G.A. 2006. Analysis of biofilm formation by Escherichia coli serotype O157:H7. Meeting Abstract.
MUTATIONS IN CRP/FNR FAMILY OF REGULATORY GENES IN LISTERIA MONOCYTOGENES STRAIN F2365 DO NOT ALTER HEAT RESISTANCE AT 60 DEGREES C - (Peer Reviewed Journal)
Bayles, D.O., Uhlich, G.A. 2006. Mutations in crp/fnr family of regulatory genes in listeria monocytogenes strain f2365 do not alter heat resistance at 60 degrees c. Journal of Food Protection. 69:2758-2760.
COMPARING TECHNIQUES FOR COMPARATIVE PROTEOMICS: TWO-DIMENSIONAL GEL ELECTROPHORESIS AND TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY SEPARATION - (Abstract Only)
Gunther, N.W., Hoan-Jen, P., Nunez, A., Uhlich, G.A. 2006. Comparing techniques for comparative proteomics: two-dimensional gel electrophoresis and two-dimensional liquid chromatography separation. Society of Industrial Microbiology. {Abstract}. P75.
EFFECT OF STORAGE TEMPERATURE ON THE VIABILITY OF LISTERIA MONOCYTOGENES IN QUESO BLANCO - (Peer Reviewed Journal)
Uhlich, G.A., Luchansky, J.B., Tamplin, M.L., Molina-Corral, F.J., Anandan, S., Porto Fett, A.C. 2006. Effect of storage temperature on the viability of listeria monocytogenes in queso blanco. Journal of Food Safety. 26:202-214.
TRANSPOSON MUTAGENESIS REVEALS POTENTIAL SUPPRESSORS OF CURLI FIBERS IN EHEC STRAIN EDL933 - (Abstract Only)
Uhlich, G.A., Gunther, N.W. 2006. Transposon mutagenesis reveals potential suppressors of curli fibers in ehec strain edl933. {Abstract}. Society for Industrial Microbiology. P.82.
EFFECT OF STORAGE TEMPERATURE ON VIABILITY OF LISTERIA MONOCYTOGENES IN QUESO BLANCO - (Abstract Only)
Uhlich, G.A., Luchansky, J.B., Tamplin, M.L., Molina-Corral, F., Anandan, S., Porto Fett, A.C. 2006. Effect of storage temperature on viability of listeria monocytogenes in queso blanco. [Abstract] 12th Australian Food Microbiology Conference.
CHARACTERIZATION, TYPING, AND DETECTION OF PATHOGENIC ESCHERICHIA COLI - (Proceedings)
Fratamico, P.M., Uhlich, G.A., Liu, Y. 2005. Characterization, typing, and detection of pathogenic escherichia coli. UJNR Food & Agricultural Panel Proceedings.
A PRFA TRANSPOSON MUTANT OF LISTERIA MONOCYTOGENES F2365, A SEROTYPE 4B STRAIN, IS ABLE TO SURVIVE IN THE GASTROINTESTINAL TRACT BUT NOT CAUSE SYSTEMIC INFECTION OF THE SPLEEN AND LIVER OF INTRAGASTRICALLY INOCULATED MICE - (Peer Reviewed Journal)
Faith, N., Uhlich, G.A., Luchansky, J.B., Neudeck, B., Czuprynski, C. 2005. A prfa transposon mutant of listeria monocytogenes f2365, a serotype 4b strain, is able to survive in the gastrointestinal tract but not cause systemic infection of the spleen and liver of intragastrically inoculated mice. Infection and Immunity. 73:7517-7524.
Analyses of the putative Crp/Fnr family of transciptional regulators of a serotype 4B strain of Listeria monocytogens - (Peer Reviewed Journal)
Uhlich, G.A., Wonderling, L., Luchansky, J.B. 2005. Transposon mutagenesis of a crp/fnr family of regulators from a serotype 4b strain of listeria monocytogenes. Food Microbiology. 23(2006):300-3006.
ANALYSES OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES - (Peer Reviewed Journal)
Uhlich, G.A., Wonderling, L., Luchansky, J.B. 2005. Analyses of the putative crp/fnr family of transcriptional regulators of a serotype 4b strain of listeria monocytogenes. Food Microbiology. Available: http://www.sciencedirect.com/science.
MUTATIONS IN CRP/FNR FAMILY OF REGULATORY GENES IN LISTERIA MONOCYTOGENES DO NOT ALTER THE HEAT RESISTANCE OF THE PATHOGEN - (Abstract Only)
Bayles, D.O., Uhlich, G.A. 2005. Mutations in crp/fnr family of regulatory genes in listeria monocytogenes do not alter the heat resistance of the pathogen. Meeting Abstract. Poster P1-11. pg. 69.
"OHMICS" AND CONTROL OF FOOD-BORNE PATHOGENS: EMPHASIS ON LISTERIA MONOCYTOGENES IN READY-TO-EAT MEATS - (Proceedings)
Uhlich, G.A., Bayles, D.O., Gilbreth, S.E., Call, J.E., Luchansky, J.B. 2004. "ohmics" and control of food-borne pathogens: emphasis on listeria monocytogenes in ready-to-eat meats. United States Japan Natural Resources Protein Panel. Food and Agriculture Panel. p. 397-401
COMPARISON OF VIRULENCE IN MICE AND CACO-2 CELL INVASION BY MUTANTS OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES - (Abstract Only)
Czuprynski, C., Uhlich, G.A., Wonderling, L.D., Faith, N., Neudeck, B., Loeb, J., Luchansky, J.B. 2004. Comparison of virulence in mice and caco-2 cell invasion by mutants of the putative crp/fnr family of transcriptional regulators of a serotype 4b strain of listeria monocytogenes. Meeting Abstract.
WHOLE GENOME COMPARISONS OF SEROTYPE 4B AND 1/2A STRAINS OF THE FOOD-BORNE PATHOGEN LISTERIA MONOCYTOGENES REVEAL NEW INSIGHTS INTO THE CORE GENOME COMPONENTS OF THIS SPECIES - (Peer Reviewed Journal)
Nelson, K., Fouts, D., Mongodin, E., Fraser, C., Kathariou, S., Wonderling, L.D., Uhlich, G.A., Bayles, D.O., Luchansky, J.B. 2004. Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen listeria monocytogenes reveal new insights into the core genome components of this species. Proceedings Of The National Academy Of Sciences. 2004. Vol. 32. No. pg. 2386-2395.
FUNCTIONAL CHARACTERIZATION OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS OF A LISTERIA MONOCYTOGENES SEROTYPE 4B STRAIN - (Abstract Only)
Uhlich, G.A., Wonderling, L., Faith, N., Neudeck, B., Loeb, J., Czuprynski, C., Luchansky, J.B. 2004. Functional characterization of the putative crp/fnr family of transcriptional regulators of a Listeria monocytogenes serotype 4b strain. Meeting Abstract. P174.
WHOLE GENOME SEQUENCING OF LISTERIA MONOCYTOGENES F2365, A SEROTYPE 4B STRAIN, AND COMPARISON TO OTHER L. MONOCYTOGENES STRAINS - (Abstract Only)
Fouts, D.E., Kolonay, J., Forberger, H.A., Khouri, H.M., Lindsay, J.A., Luchansky, J.B., Tran, B., Uhlich, G.A., Wonderling, L.D., Nelson, K.E. 2003. Whole genome sequencing of listeria monocytogenes f2365, a serotype 4b strain, and comparison to other l. monocytogenes strains. American Society of Microbiology. Abstract # R-058, p. 621.
PHENOTYPIC ANALYSES OF THE PUTATIVE CRP/FNR FAMILY OF TRANSCRIPTIONAL REGULATORS IN A SEROTYPE 4B STRAIN OF LISTERIA MONOCYTOGENES - (Abstract Only)
Uhlich, G.A., Wonderling, L.D., Luchansky, J.B. 2003. Phenotypic analyses of the putative crp/fnr family of transcriptional regulators in a serotype 4b strain of listeria monocytogenes. American Society for Microbiology. Abstract #K-125, p. 381.
IDENTIFICATION OF TWO PUTATIVE REGULATORY GENES REQUIRED FOR MOTILITY OF LISTERIA MONOCYTOGENES STRAIN F2365 - (Abstract Only)
Wonderling, L.D., Uhlich, G.A., Cooke, P.H., Luchansky, J.B. 2003. Identification of two putative regulatory genes required for motility of listeria monocytogenes strain f2365. American Society of Microbiology.Abstract # K126, p. 381.
ENVIRONMENTAL, HOST, AND BACTERIAL FACTORS THAT INFLUENCE THE PATHOGENESIS OF GASTROINTESTINAL LISTERIOSIS - (Proceedings)
Czuprynski, C.J., Faith, N.G., Tamplin, M.L., Uhlich, G.A., Wonderling, L.D., Bayles, D.O., Luchansky, J.B. 2003. Environmental, host, and bacterial factors that influence the pathogenesis of gastrointestinal listeriosis. Meeting Abstract. Part 8,Section 15, No.3.
VARIATIONS IN THE CSGD PROMOTER OF ESCHERICHIA COLI O157:H7 ASSOCIATED WITHINCREASED VIRULENCE IN MICE AND INCREASED INVASION OF HEP-2 CELLS - (Peer Reviewed Journal)
Infection and Immunity. 2002. v. 70. p. 395-399.
MUTATIONS IN THE CSGD PROMOTER ASSOCIATED WITH VARIATIONS IN CURLI EXPRESSION IN CERTAIN STRAINS OF ESCHERICHIA COLI O157:H7 - (Peer Reviewed Journal) - (16-Feb-01)
PHASE VARIATION OF CURLI EXPRESSION IN STRAINS OF ESCHERICHIA COLI O157:H7 - (Abstract Only) - (04-Jul-00)
EFFECTS OF HAY- AND GRAIN-BASED DIETS ON FECAL SHEDDING OF NATURALLY- ACQUIRED ENTEROHEMORRHAGIC ESCHERICHIA COLI (EHEC) O157 IN BEEF FEEDLOT CATTLE - (Abstract Only) - (07-Oct-99)
ARS News Articles
Last Modified: 9/1/2014
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