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United States Department of Agriculture

Agricultural Research Service

Glenn R Welch
Animal Biosciences and Biotechnology Laboratory
Biologist

Phone: (301) 504-9282
Fax: (301) 504-5123
Room 113

BLDG 200 BARC-EAST
BELTSVILLE, MD, 20705-2350


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Comparison of methods of extracting messenger Ribonucleic Acid from ejaculated Porcine (Sus Scrofa) Spermatozoa - (Abstract Only)
Frankel, T.E., Theisen, D.D., Guthrie, H.D., Welch, G.R., Woods L.C. III. 2013. The effect of freezing rate on the quality of striped bass sperm. Theriogenology. 79(6):940-945. DOI:10.1016/j.theriogenology.2013.01.099.
The effect of freezing rate on the quality of Striped Bass Spermatozoa - (Peer Reviewed Journal)
Frankel, T.E., Theisen, D.D., Guthrie, H.D., Welch, G.R., Woods, III, L.C. 2013. The effect of freezing rate on the quality of Striped Bass Spermatozoa. Theriogenology. 79(6):940-945.
Isolation of RNA striped bass Monrone saxatilis spermatozoa: Implications for teleost male fertility and beyond? - (Abstract Only)
Woods, L.C., Guthrie, H.D., Welch, G.R., Fuller, S.A. 2013. Isolation of RNA striped bass Monrone saxatilis spermatozoa: Implications for teleost male fertility and beyond[abstract]? Book of Abstracts Aquaculture America 2013: Strike a Chord for Sustainable Aquaculture, February 21-25, 2013, Nashville, Tennessee. p.1184.
Isolation of RNA from striped bass (Morone saxatilis) spermatozoa: implications for teleost male fertility and beyond? - (Abstract Only) - (31-Aug-12)
Effects of reactive oxygen species action on sperm function in spermatozoa - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R. 2012. Effects of reactive oxygen species action on sperm function in spermatozoa. Theriogenology. 78(8):1700-1708.
Quality assessment of wild Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) semen under conditions of short-term storage - (Peer Reviewed Journal)
Dorsey, K.M., Guthrie, H.D., Welch, G.R., Mohler, J., Theisen, D.D., Siewerdt, F., Vinyard, B.T., Woods, C.L. 2011. Quality assessment of wild Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) semen under conditions of short-term storage. North American Journal of Aquaculture. 73:418-425.
Effects of hypothermic storage of striped bass (Morone saxatilis) sperm on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and viability - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R., Theisen, D., Woods, L.C. 2011. Effects of hypothermic storage of striped bass (Morone saxatilis) sperm on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and viability. Theriogenology. 75:951-961.
Effects of hypothermic storage of striped bass (Morone saxatilis) sperm on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and viability - (Abstract Only)
Guthrie, H.D., Woods, L.C., Welch, G.R. 2010. Effects of hypothermic storage of striped bass (Morone saxatilis) sperm on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and viability. Poultry Science Association Meeting Abstract.
A Protocol for the Short-Term Storage of Atlantic Sturgeon Semen - (Abstract Only)
Woods, III, L.C., Dorsey, K., Mohler, J., Guthrie, H.D., Welch, G.R. 2010. A PROTOCOL FOR THE SHORT-TERM STORAGE OF ATLANTIC STURGEON SEMEN. Meeting Abstract.
Short-term storage of Atlantic sturgeon spermatozoa - (Abstract Only)
Woods, L.C., Dorsey, K., Mohler, J., Guthrie, H.D., Welch, G.R. 2009. Short-term storage of Atlantic sturgeon spermatozoa. American Fisheries Society Annual Meeting.
Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) using flow cytometry. - (Book / Chapter)
Guthrie, H.D., Welch, G.R. Determination of high mitochondrial membrane potential in spermatozoa loaded with the mitochondrial probe 5,5',6,6'tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) using flow cytometry.. Book Chapter. Methods in Molecular Biology, vol. 477: Advanced Protocols for Oxidative Stress I, Ed. D. Armstrong, DOI: 10.1007/978-1-60327-517-2_1, Humana Press New York, NY 07512. 2008. pp. 89-97
Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa - (Book / Chapter)
Guthrie, H.D., Welch, G.R. 2010. Using fluorescence-activated flow cytometry to determine reactive oxygen species formation and membrane lipid peroxidation in viable boar spermatozoa. Methods in Molecular Biology (Advanced Protocols in Oxidative Stress II). 594:163-171.
Effects of osmolality on testicular striped bass (Morone saxatilis) sperm energy status - (Peer Reviewed Journal)
Guthrie, H.D., Woods, L.C., III, Long, J.A., Welch, G.R. 2008. Effects of osmolality on inner mitochondrial transmembrane potential and ATP content in spermatozoa recovered from the testes of striped bass (Morone saxatilis). Theriogenology. 69:1007-12.
Opportunities to improve liquid and frozen storage of boar semen - (Abstract Only)
Guthrie, H.D., Welch, G.R. 2008. Opportunities to improve liquid and frozen storage of boar semen. Journal of Animal Science, 86, E-Suppl. 3: 45-46.
Mitochondrial function and reactive oxygen species action in relation to boar motility. - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G., Long, J.A. 2008. Mitochondrial function and reactive oxygen species action in relation to boar motility. Theriogenology. 70:1209-1215.
Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with C11-BODIPY 581/591 - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R. 2007. Use of fluorescence-activated flow cytometry to determine membrane lipid peroxidation during hypothermic liquid storage and freeze-thawing of viable boar sperm loaded with C11-BODIPY 581/591. Journal of Animal Science. 85:1402-1411.
Determination of Intracellular Reactive Oxygen Species and High Mitochrondrial Membrane Potential in Viable Boar Sperm Using Fluorescence Activated Flow Cytometry - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R. 2006. Determination of intracellular reactive oxygen species and high mitochrondrial membrane potential in viable boar sperm using fluorescence activated flow cytometry. Journal of Animal Science. 84:2089-2100.
Treatment of Boar Sperm with Respiration Inhibitor Menadione: Effects on Motility, Reactive Oxygen Species (Ros), Mitochondrial Transmembrane Potential (Mmp), and Atp Content - (Peer Reviewed Journal)
Guthrie, H.D., Long, J.A., Welch, G.R., Grimm, N.E. 2006. Treatment of boar sperm with respiration inhibitor menadione: effects on motility, reactive xygen species (ros), mitochondrial transmembrane potential (mmp), and atp content [abstract]. Reproduction, Fertility and Development. 18:259-260.
New Assays for Reactive Oxygen Species (Ros) Formation and Mitochondrial Membrane Potential in Fresh and Cryopreserved Boar Spermatozoa - (Abstract Only)
Guthrie, H.D., Welch, G.R. 2005. New assays for reactive oxygen species (ros) formation and mitochondrial membrane potential in fresh and cryopreserved boar spermatozoa [abstract]. 7th International Conference on Pig Reproduction National Conference. p. 141.
Effects of Hypothermic Liquid Storage and Cryopreservation on Basal and Induced Plasma Membrane Phospholipid Disorder and Acrosome Exocytosis in Boar Spermatozoa - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R. 2005. Effects of hypothermic liquid storage and cryopreservation on basal and induced plasma membrane phospholipid disorder and acrosome exocytosis in boar spermatozoa. Reproduction, Fertility and Development. 17:467-477.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Peer Reviewed Journal)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Dawson, H.D., Welch, G.R., Garrett, W.M. 2005. Follicular expression of a human B-cell leukemia/lymphoma-2 (Bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Journal Of Reproduction, Fertility And Development. 17(4):457-468.
Impact of Extender and Cryopreservation on Membrane and Morphological Integrity in Boar Sperm - (Proceedings)
Guthrie, H.D., Welch, G.R. 2004. Impact of extender and cryopreservation on membrane and morphological integrity in boar sperm. Theriogenology. 63:396-410.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Abstract Only)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine [abstract]. Society for the Study of Reproduction Annual Meeting. p. 275.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine. - (Abstract Only)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Society for the Study of Reproduction Annual Meeting. Abstract. p275.
Cag Repeat Lengths in X and Y-Bearing Sperm Indicate That Gender Bias During Transmission of Huntington's Disease Gene Is Determined in the Embryo. - (Peer Reviewed Journal)
Kovtun, I.V., Welch, G.R., Guthrie, H.D., Hafner, K.L., Mcmurray, C.T. 2004. Cag repeat lengths in x- and y-bearing sperm indicate that gender bias during transmission of huntington's disease gene is determined in the embryo. Journal of Biological Chemistry. Vol. 279, No. 10, Issue of March 5, pp. 9389-9391.
Offspring Sex Ratio in Deer Male Reproductive Physiology and Offspring Sex Ratio in Odocoileus Virginianus. - (Peer Reviewed Journal) - (12-Sep-03)
Increasing the Holding Time at 15 C of Boar Spermatozoa Before Freezing Decreases Fertility after Thawing. - (Peer Reviewed Journal)
Guthrie, H.D., Welch, G.R. 2003. Increasing the holding time at 15 c of boar spermatozoa before freezing decreases fertility after thawing. [abstract]. Journal of Animal Science. 81(2):88.
Effects of Osmotic Stress and Bovine Serum Albumin on Sperm Motion Characteristics and Plasma Membrane Integrity in Boars - (Abstract Only) - (12-Apr-02)
Flow Cytometric Sperm Sorting: Effects of Varying Laser Power on Embryo Development in Swine - (Peer Reviewed Journal) - (02-Jul-01)
Influence of Dibutyrl Camp on Fertilization with Sexed Sperm - (Abstract Only) - (10-Oct-00)
Low Dose Artificial Insemination of Swine with Sperm Sorted for Sex Using An Intrauterine Technique in Sows - (Abstract Only) - (08-May-00)
Cryopreservation of Flow Cytometrically Sorted Boar Sperm: Effects on in Vivo Embryo Development - (Abstract Only) - (08-May-00)
Flow Cytometric Sperm Sorting: Effects of Varying Laser Power on Embryo Development in Swine - (Abstract Only) - (03-Apr-00)
Sex Preselection in Swine: Flow Cytometric Sorting of X and Y Chromosome Bearing Sperm to Produce Offspring - (Peer Reviewed Journal) - (01-Jan-00)
Sex Preselection: Laboratory Validation of the Sperm Sex Ratio of Flow Sorted X and Y-Sperm by Sort Reanalysis for DNA - (Peer Reviewed Journal) - (13-Oct-99)
Sex Preselection: High Speed Flow Cytometry Sorting of X and Y Sperm for Maximum Efficiency - (Peer Reviewed Journal) - (13-Oct-99)
In Vitro Production of Sexed Embryos for Gender Preselection: High Speed Sorting of X-Chromosome Bearing Sperm to Produce Piglets after Embryo Transfer - (Peer Reviewed Journal) - (26-May-99)
The Relationship Between Membrane Status and Fertility of Boar Spermatozoa after Flow Cytometric Sorting in the Presence Or Absence of Seminal Plasma - (Peer Reviewed Journal) - (20-Mar-99)
Substantial Increase in Yield of Sexed Semen by Improved Flow Cytometric Sperm Separation Efficiency - (Peer Reviewed Journal) - (21-Jul-98)
Birth of Piglets Preselected for Gender Following in Vitro Fertilization of in Vitro Matured Pig Oocytes by X and Y-Chromosome Bearing Spermatozoa Sorted by High Speed Flow Cytometry - (Peer Reviewed Journal) - (24-Jun-98)
Rapid Estimation of Flow Sorted X and Y-Bearing Sperm by Fluorescence in Situ Hybridization - (Peer Reviewed Journal) - (15-Jun-98)
A Novel Nozzle for Faster Flow Cytometric Analysis of Sperm DNA Content. - (Peer Reviewed Journal) - (01-Jun-98)
Comparative Motility of X and Y-Chromosome Bearing Bovine Sperm Separated on the Basis of DNA Content by Flow Sorting - (Peer Reviewed Journal) - (23-Jan-98)
Uterine Horn Insemination of Heifers with Very Low Numbers of Nonfrozen Sperm and Sexed Sperm - (Peer Reviewed Journal) - (21-May-97)
Production of Piglets Preselected for Sex Following in Vitro Fertilization with Flow Cytometrically Sorted X and Y Chromosome Bearing Sperm - (Peer Reviewed Journal) - (24-Oct-96)
Sex Preselection in Mammals by Dna: a Method for Flow Separation of X and Yspermatozoa in Humans - (Book / Chapter) - (12-Sep-96)
Viability and Membrane Integrity of Spermatozoa after Dilution and Flow Cytometric Sorting in the Presence Or Absence of Seminal Plasma - (Peer Reviewed Journal) - (26-Aug-96)
Slit-Scan Flow Cytometry for Consistent High Resolution DNA Analysis of X and Y-Chromosome Bearing Sperm - (Peer Reviewed Journal) - (08-Jun-96)
Porcine Sperm Viability, Oocyte Fertilization and Embryo Development after Staining Sperm with Sybr-14 - (Peer Reviewed Journal) - (18-Oct-95)
Flow Cytometric Sperm Sorting and Pcr to Confirm Separation of X and Y Chromosome Bearing Bovine Sperm - (Peer Reviewed Journal) - (29-Sep-95)
Atresia in the First Wave of Follicular Growth after Ovulation in the Pig: Measurement of Increased Apoptosis in Granulosa Cells and Reduced Follicular Fluid Estradiol - (Peer Reviewed Journal) - (20-Sep-94)
Last Modified: 7/31/2014
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