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United States Department of Agriculture

Agricultural Research Service


Location: Plant, Soil and Nutrition Research

2005 Annual Report

4d.Progress report.
This report serves to document research conducted under a specific cooperative agreement between ARS and the Department of Microbiology at Cornell University. Additional details of research can be found in the report for the parent project 1907-31000-005-00D Alternative Mechanisms for Improving Ruminal Fermentation.

This project began in late 2004. The objective is to isolate and identify ruminal bacteria responsible for ruminal lysine degradation and to study the effect of the feed additive, monensin, and bacteriocins on lysine degradation. Lysine is an amino acid that often limits milk production, but bacteria responsible for wasteful ruminal lysine degradation had not yet been identified. We enriched mixed ruminal bacteria with lysine in vitro, and isolated strains capable of utilizing lysine as their sole energy source, characterized physiologically and identified via 16S rDNA sequencing as a strain of Fusobacteria necrophorum. In the next year will give cattle a lysine supplement to see if Fusobacteria necrophorum is enriched in vivo.

Last Modified: 4/18/2014
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