Start Date: Jun 03, 2005
End Date: Jun 02, 2010
For the first objective on rearing technology, we propose to examine various methods including: refinement of the existing defined larval and adult diets, new diet bulking agents, and sugar substitutes. The approach will be to compare new diets formulated based on new knowledge of nutrition, vitiamin requirements and bulking agents with the existing diets used for mass-rearing of flies. Data to be collected will include developmental time, days from egg to pupation, overall yield and size of larvae and pupae at different developmental stages. This work will focus on the Mediterranean fruit fly initially, then later on other fruit fly (esp. Bactrocera) or parasitoid species. For the second objective on quality control, we propose to emphasize evaluations of flies for field cage mating competitiveness and survival ability, and of parasitoids for field cage parasitism rates and survival ability. This will be accomplished by visaul observation of fly competitiveness and behavioral characteristics (including mating) in standard field cages. Data from different strains will be compared with currently mass-reared laboratory strains and wild field strains.For the third objective, standard genetic tools will be used for developing new genetic sexing strains in the Bactrocera, especially for B. latifrons. Standard gentetic tools will include pairing of wild and lab strains, and low dose irradiation for translocation lines that might reveal superior traits to the existing strains. The existing melon fly and oriental fruit fly genetic sexing strains will continue to be field evaluated in current or new sterile insect release programs in Hawaii or elsewhere in the Pacific or Asia. Formerly 5320-22430-019-00D (4/05).