Start Date: Oct 01, 2005
End Date: Sep 30, 2010
Population genetic studies for characterizing natural populations of H. virescens and H. zea are in need of a comprehensive set of genetic markers. Well defined genetic markers will also facilitate the development of linkage maps which in turn will serve as valuable tools in studying inheritance of traits. Simple sequence repeats (SSR) or microsatellites found in genomes as well as single nucleotide polymorphisms (SNP) found in gut specific genes will be used as genetic markers for H. virescens and H. zea. Genomic DNA libraries enriched for microsatellite sequences will be used to develop SSR markers. Subtraction cDNA libraries enriched for gut specific genes will be used to identify SNP markers.