MOLECULAR, NUTRIENT, AND ENDOCRINE BASIS OF METABOLIC DISEASES THAT AFFECT THE REPRODUCTIVE HEALTH OF THE DAIRY COW
Project Number: 3625-32000-085-00
Start Date: Nov 15, 2006
End Date: Nov 14, 2011
Improve the efficacy of diets to prevent clinical and subclinical hypocalcemia in the periparturient dairy cow.
Use of genomic and proteomic approaches to identify genes in the kidney, intestine, and/or mammary gland that are modulated by vitamin D metabolites and dietary cation-anion difference (DCAD) diets to better understand the molecular basis of disease and develop effective intervention strategies to prevent hypocalcemia in the transition cow.
Identify the pathways (genes/proteins) responsible for calcium entry, calcium storage, and calcium transport in the mammary gland as well as points of regulation for these calcium transport and storage mechanisms. Determine primary factors responsible for excess mammary calcium drain in animals with hypocalcemia.
The approach to this research is a combination of applied and basic investigations to obtain new knowledge that will increase understanding and result in practical solutions for the prevention of subclinical a clinical hypocalcemia in dairy cows. The research will investigate the mechanism of action of DCAD diets in the prevention of hypocalcemia. Agronomic research will be used to develop forages that are low in DCAD thus providing more palatable approaches to feeding low DCAD diets for prevention of hypocalcemia. Basic research approaches will be employed to gain an understanding of role and contribution of mammary calcium transport in the development of clinical and subclinical hypocalcemia in cows. IBC-0227, BSL-2, Exempt; recertified 10/17/08; IBC-0305, BSL-1, Approved 6/11/09.