Start Date: May 14, 2008
End Date: May 13, 2013
Objective 1: We will map genomic regions and attempt to identify candidate genes for morphological phenotypes that may have changed in response to selection for agronomic performance in the Iowa Stiff Stalk Synthetic population. We will focus on three morphological phenotypes, silking-anthesis interval, leaf angle, and number of ears per plant. We will determine the magnitude of selection response for these traits in Iowa Stiff Stalk Synthetic population and determine if any of these are correlated to agronomic traits in the base population. We will then use advanced cycles of selection and the base population to generate a genetic mapping experiment to determine how many regions and what magnitude of effects those regions have for the morphological traits. We will use all of the information collected to determine if response to selection for agronomic traits can be explained by indirect response for morphological characters. Objective 2: One method of production of biofuel from plant material involves fermentation, which is dependant on the production of sugars from the plant material. We will develop methods for screening varieties for their ability to produce sugars in small scale processes that mimic current methods being used for production of ethanol. Sugars will be quantified using bacterial biosensor strains we will develop using bacterial genes known to respond to sugar levels. Objective 3: We will characterize the genetic mechanism controlling production of methionine by biochemical analysis of populations selected for high and low methionine. In addition, we will determine if different genetic mechanisms controlling methionine levels are complimentary by combining them genetically and determining the methionine levels in the different genetic combinations.