TRANSLATIONAL RESEARCH AND BREEDING IN SWITCHGRASS
USING MAIZE AS A MODEL DISCOVERY ENGINE
Project Number: 3655-41000-006-06
Nonfunded Cooperative Agreement
Start Date: Nov 01, 2008
End Date: Oct 31, 2013
1. Germplasm characterization:
a. Develop DNA markers that can be used as tools to select switchgrass plants with superior performance for Biofuel and bioenergy uses.
b. Develop selection methods and models in which DNA markers can be used to select the best switchgrass plants in segregating populations and as parents for upland X lowland hybrids.
2. Translational research and breeding:
a. Identify functional polymorphisms in switchgrass genes that are directly associated with important traits for Biofuel and bioenergy uses.
b. Identify candidate genes from maize, sorghum, Setaria, and other species that are homologous to important polymorphic regions in switchgrass, describe the function of these genes, and develop generalized markers that can be used to implement marker selection in switchgrass.
1. Conduct amplification and polymorphism analyses using EST-SSR markers on switchgrass association panels, including marker screening, scoring, and analysis.
2. Conduct simulation studies on marker-assisted selection methodologies to optimize the deployment of both laboratory and field facilities to maximize genetic gain per unit of expenditure.
3. Develop SNP markers from sequence analysis of candidate regions.
4. Develop methods by which performance of both half-sib and full-sib family performance can be predicted from genotypic assessment of parents.