2009 Annual Report
To improve the transmissibility of Iris yellow spot virus (IYSV) by manual means, a new set of indicator hosts were identified which should be useful as a source of inoculum for infecting onion for the purpose of developing a mechanical screening procedure to evaluate the response of onion cultivars and breeding lines to infection by IYSV. Findings are accepted for publication in the referred journal, Plant Health Progress, published by the American Phytopathological Society. (2009. Symptomatology of Iris yellow spot virus in selected indicator hosts. Plant Health Progress. In press). Various mechanical inoculation protocols to improve the transmission efficiency of IYSV to healthy onion are in progress. Onion thrips (Thrips tabaci) colony is established so that screening by viruliferous thrips could also be attempted. An Enzyme-linked immunosorbent assay (ELISA) was developed for determining the viruliferous thrips for this purpose. Project is monitored by conference calls every two months.