Project Number: 6618-41000-015-06
Project Type: Nonfunded Cooperative Agreement

Start Date: Mar 11, 2010
End Date: Mar 10, 2015

Objective:
1. Measure in vivo pharmacokinetics and biological actions of citrus phytonutrients in human and small-animal trials. 2. Study factors that influence the in vivo pharmacokinetics of citrus phytonutrients. 3. Characterize the beneficial actions of citrus phytonutrients in in vivo systems.

Approach:
1. Administer citrus juices to test subjects and to monitor targeted biological actions and the presence of these compounds or their metabolites in biological sample extracts, primarily of blood and urine, over set periods of time. 2. Extract phytonutrients and main metabolites from biological tissue extracts, and analyze by high performance liquid chromatography-mass spectrometry (HPLC-MS).