Start Date: Feb 15, 2010
End Date: Feb 14, 2015
In a previous study we generated polyclonal antibodies against several pestivirus species including BVDV1, BVDV2, and classical swine fever virus. Based on cross neutralization studies it was possible to differentiate the serum antibody response raised against different pestivirus species. In the initial phase of this collaboration we will generate polyclonal antibodies against a HoBi-like virus strain and compare the ability of this serum to bind viruses from other pestivirus species. If we can differentiate antibodies raised against HoBi-like viruses from antibodies raised against BVDV1 and BVDV2, the next step will be to develop assays based on this observation that can be used in the field. The generation of polyclonal antisera and development of the differential serum antibody test will be conducted at the NADC. A graduate student from the Federal University of Santa Maria will spend a six-month sabbatical at the NADC to assist in this research. The next step will be to use the test to survey Brazilian and U.S. cattle sera. Surveillance testing will be done both at the NADC and at the Federal University of Santa Maria. If the serum survey indicates that animals in Brazil or the U.S. have significant exposure to HoBi-like strains we will develop a test that can be used to screen for animals persistently infected with HoBi. Development of this test will be a joint effort between the NADC and the Federal University of Santa Maria. Any HoBi-like virus isolated from field cases in the course of these studies will be characterized and sequenced.