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United States Department of Agriculture

Agricultural Research Service

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Research Project: Identification and Mapping of Disease Resistance Genes in Wheat

Location: Cereal Crops Research

Project Number: 3060-21000-037-16
Project Type: Specific Cooperative Agreement

Start Date: Nov 16, 2009
End Date: Oct 31, 2014

The objectives of this cooperative research project are: 1) identify tan spot and Stagonospora nodorum blotch resistance genes in wheat, 2) determine the chromosomal locations of the resistance genes, and 3) identify or develop markers suitable for marker-assisted selection of the resistance genes.

A recombinant inbred (RI) population derived from a cross between the resistant wheat landrace Salamouni and the susceptible Canadian variety Katepwa will be developed for the identification and mapping of novel resistance genes. The RI population will be genotyped with molecular markers, and linkage maps representing all 21 wheat chromosomes will be assembled. The population of RI lines will be screened for reaction to multiple races of the tan spot fungus and isolates of S. nodorum. The phenotypic data will be regressed on the molecular marker data to identify quantitative trait loci (QTL) associated with resistance. Genomic regions harboring significant QTL will be targeted for saturation mapping to identify and/or develop user-friendly PCR-based markers tightly linked to the QTL for marker-assisted selection.

Last Modified: 10/30/2014
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