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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development and Evaluation of Diagnostic Applications for Arboviruses

Location: Arthropod-Borne Animal Diseases Research

Project Number: 3020-32000-006-04
Project Type: Nonfunded Cooperative Agreement

Start Date: May 01, 2010
End Date: Apr 30, 2015

Objective:
The long term goal of this project is the development of rapid, reliable, and sensitive diagnostic assays for the identification of insect transmitted viruses, arboviruses, that are suitable for use in veterinary diagnostic laboratories, as well as in the field for use during times of natural or intentionally caused disease epidemics.

Approach:
The ARS and KSU have and are developing assays for detecting genetic material, antigen or antibodies to viruses using a variety of diagnostic formats on a manual laboratory scale. The approach is to evaluate these assays on diagnostic specimens in conjunction with the standard assays used in the veterinary diagnostic laboratory. Those assays that are found to be acceptable then will be further optimized and automated for a high-throughput scale. The primary focus of the proposed studies will be the demonstration and evaluation of new diagnostic assays for arboviruses, followed by the translation of these to validated tests to be used by veterinary diagnosticians.

Last Modified: 10/24/2014
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