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United States Department of Agriculture

Agricultural Research Service

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Research Project: Coordinated Analysis of Soybean Breeding Germplasm

Location: Bio-oils Research Unit

2013 Annual Report


1a.Objectives (from AD-416):
Develop cutting-edge analytical standards to provide a platform for essential activities to support soybean composition improvement in the U.S., including amino acids, fatty acids, protein, oil, and other important constituents.


1b.Approach (from AD-416):
Using new wet chemical methods, improved near infrared calibrations will be performed and compared to calibrations by other NIR users. A wider range of soybean composition will be worked into the calibration. Soxhlet and nitrogen analyses from newly obtained equipment will be used in the calibration. Carbohydrate analyses will be performed on a large number of soybean samples and added to the current calibration.


3.Progress Report:
ARS scientists in the Bio-Oils Research Unit at the USDA-ARS National Center for Agricultural Utilization Research (NCAUR), Peoria, Illinois, conducted 6,732 near infrared (NIR) analyses for protein, oil, and moisture content for the annual coordinated soybean effort within ARS. This work supported the efforts of 39 ARS researchers. In addition, the unit performed 3,925 NIR and pulsed nuclear magnetic resonance (NMR) analyses for three individual ARS soybean breeding programs. This work required the use of pulsed NMR for total oil and total nitrogen content due to the presence of off-colored soybeans which did not yield reliable NIR results. An upgraded total nitrogen analyzer was purchased this year which supports NIR calibrations for protein composition.


4.Accomplishments
1. Analytical analysis of soybeans. ARS soybean breeders are developing soybean varieties with improved traits for disease resistance and improved oil and protein quality. During these breeding efforts, chemical confirmation of trait improvements or preservation of desired chemical profiles are necessary. In this effort to confirm chemical trait improvement, 18,657 ARS samples submitted by soybean breeders have been analyzed by ARS scientists in the Bio-Oils Research Unit at the USDA-ARS National Center for Agricultural Utilization Research (NCAUR), Peoria, Illinois, and the results reported to the respective researchers. As a result of this sampling, we have improved the quality of soybeans for disease resistance and oil/protein content.


Last Modified: 8/29/2014
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