Project Number: 6402-22000-062-02
Start Date: May 15, 2010
End Date: May 14, 2015
Development of chemically defined colloidal artificial diets, designed to resemble different plant hosts, will involve detailed chemical analyses of the plants by High Performance Liquid Chromatography (HPLC), Gas Chromatography (GC), Mass Spectrometry (MS), and Spectrophotometry. Based on the analysis, chemically defined artificial diets will be prepared by ARS. L. lineolaris and L. hesperus fed on the artificial diet formulations will be evaluated against L. lineolaris and L. hesperus reared on natural food sources, under controlled environmental conditions. Artificial diet formulations will be evaluated by measure of comparison of biological parameters such as developmental time, survivorship, sex ratio, fecundity, egg viability and longevity. Once an acceptable, colloidal formulation is identified biological proteins provided by cooperator will be incorporated in the formulation. The formulation then provided as diet to L. lineolaris and L. Hesperus and evaluated for deleterious effects on insect survival. Additionally, these biological proteins will be incorporated into water retaining agents at different concentrations to determine repellency levels. Development of the artificial diet based mass rearing systems will involve the optimization of mass production, dispensing, and egg collection.