Location: Food and Feed Safety Research
Project Number: 3091-32000-032-00
Start Date: Mar 21, 2011
End Date: Mar 20, 2016
Objective 1: Management practices and environmental factors and their interactions on the growth of foodborne pathogens in poultry will be evaluated. Broiler chickens will be vaccinated with commercially available vaccines (e.g., Marek's) using several routes of administration and evaluated for Salmonella within the gastrointestinal tract. Beneficial bacteria will be identified from broilers infected with Salmonella and compared to the bacteria found in broilers without Salmonella using a sophisticated DNA technology called pyrosequencing. Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle which will allow the identification of hundreds of bacteria from a single sample. This will lead to the development of new interventions to help control numerous foodborne pathogens. Another approach will investigate the spread of antibiotic resistance from bacteria to bacteria, using an antibiotic (bambermycin) that prevents the transfer of genetic information. Within the environment of broilers, lesser mealworms will be exposed to numerous natural and artificial attractants for the development of new control programs. Poultry are raised on wood flakes that may be contaminated with foodborne pathogens, numerous wood products will be evaluated for their antimicrobial activity against these pathogens using pyrosequencing technology. Objective 2: New intervention strategies will be evaluated to reduce foodborne pathogen colonization in poultry that can be incorporated into existing management practices. Several compounds, including chitosan, chlorate, calcium formate, and clay montomorillonite, will be incorporated into existing commercial management programs. Broilers will be provided the products and challenged with foodborne pathogens and evaluated for the recovery of the pathogens. These compounds utilize different mechanisms for controlling foodborne pathogens, including absorption of the pathogen, targeting enzymatic pathways within the pathogens, and activation of the host immune system. Objective 3: Existing management practices will be evaluated on survival and dispersion of pathogens within the poultry facility and the surrounding environment. Comparisons of management practices on their ability to prevent the spread of pathogenic bacteria and lesser mealworm into the environment will be made using conventional bacterial culture methodology, PCR-based technology, mineral identification, and minimum inhibitory concentration (MIC) analysis. Objective 4: The role of broiler and layer fungal digestive populations in poultry production and protection against colonization by human foodborne enteropathogens will be defined. Using conventional fungal culture methodology, PCR methodology, and sequencing technology fungal species will be identified that have ability to reduce Salmonella and Campylobacter. Building on the information gained in Objective 1, fungal populations recovered in the gastrointestinal tract of broilers taken from Salmonella positive or negative farms will be compared for changes in the fungal profiles using pyrosequencing.