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United States Department of Agriculture

Agricultural Research Service

Research Project: Mir604 and 5307-Western Corn Rootworm Resistant Colonies: Follow-Up Studies

Location: Plant Genetics Research

Project Number: 5070-21220-007-07
Project Type: Trust

Start Date: Aug 01, 2010
End Date: Sep 30, 2014

Objective:
Characterize MIR604- and 5307-western corn rootworm resistant colonies.

Approach:
1) Removal of selection experiments with the MIR604 resistant colony similar to Meihls et al. (2008). 2) Reciprocal crosses of the MIR604-selected colony and its control colony for calculation of heritability. We will do both similar studies to Meihls et al. (2008) and more detailed studies in which we will control for genetic divergence between selected and unselected colonies. 3) Evaluate for fitness costs of the MIR604 resistance. 4) Probe differences between the MIR604-selected colony and a split-off colony with a lower LC50 value that survived MIR604 in the field in 2009. We will focus on differences in feeding behavior and the potential role of feeding behavior in contributing to field resistance. 5) Provide Syngenta with up to 20,000 eggs each from the MIR604-selected and its paired control colony. 6) Removal of selection experiments with the 5307-selected colony similar to Meihls et al. (2008). 7) Reciprocal crosses of the 5307-selected colony and its control colony for calculation of heritability. We will do both similar studies to Meihls et al. (2008) and more detailed studies in which we will control for genetic divergence between selected and unselected colonies. 8) Evaluate fitness costs of 5307 resistance. 9) Provide Syngenta with up to 20,000 eggs each from the 5307-selected and its paired control colony. 10) Evaluate cross-resistance between 5307 and MIR604-selected colonies in the greenhouse and field (cross-resistance with Cry3Bb1 is also possible now and with Cry34/35Ab1 will also likely be possible within a year).

Last Modified: 10/25/2014
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