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United States Department of Agriculture

Agricultural Research Service

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Research Project: Constructing a Dna-Based Systematic Framework for the Bactrocera Dorsalis Complex (Diptera:tephritidae) for Improved Control and Quarantine

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-025-03
Project Type: Specific Cooperative Agreement

Start Date: Sep 29, 2010
End Date: Aug 31, 2014

1) Produce a voucher collection of Bactrocera samples across subspecies from diverse range of populations through input from international collaborator and collecting trips. 2) Perform morphological identification of specimens and provide samples for generic analysis to develop species and population level markers. 3) Initiate species complex population studies, provide taxonomic ID of specimens, and curation of molecular data and insect specimens.

Using traditional methods of fruit fly taxonomy we will trap, curate, and identify fly specimens within the B. dorsalis species complex. This traditional work will be conducted by a post-doctoral researcher with prior training in B. dorsalis identification. Samples will be collected from the Pacific region, East Asia, and Australia and then transferred to UH Manoa for curation at the Department of Entomology Insect Museum. After morphological identification, these specimens will then be available to be genetically analyzed using currently developed phylogenetic markers and new markers identified from genome-wide analysis of reference specimens within USDA-PBARC and the University of Hawaii at Manoa labs. Formerly 5320-22430-023-07S; 6/2011.

Last Modified: 1/28/2015
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