Start Date: Sep 20, 2010
End Date: Sep 30, 2012
1. Each of three participating queen breeders will establish an apiary in a Varroa-infested area with colonies of several levels of VSH (0, 50 and 75%). The colonies will be started as splits with no sealed brood. Varroa densities on adult bees will be measured and colonies assigned to equalize mite densities among levels of VSH within each breeder’s splits. Colonies will be managed initially without Varroa treatments. Cooperator will sample colonies every two months to monitor density of Varroa, and treat individual colonies that reach a predetermined density of mites on adult bees. Participating queen breeders will rate the beekeeping characteristics of the colonies. 2. In a laboratory bioassay, mature larvae of SHB will be reared in cylinders containing one of three treatments: lava rock alone, soil alone and a combination of lava rock and soil. In the field, pupation success will be determined in at least 5 apiaries. 3. Reproductive success of SHB will be assessed in the laboratory using different fruits commonly grown in Hawaii. In the field, fresh and rotting fruits from trees in and near test apiaries also will be examined for different stages of SHB.