2012 Annual Report
5)% RH and 16:8 (L:D) light photoperiod until they started active feeding (usually about two to three weeks after field collection). Only laboratory conditioned adults were used for the insecticide bioassays. Adult BMSB were separated based on gender and grouped by five individuals. Each group was placed in a plastic 0.473 l (16.0 oz) clear plastic container supplied with a small piece of carrot (2 mm slice) as a source of food and moisture. Adult BMSB in six containers per gender (n=30 males or females) were separated and treated with a topical application of water solution of formulated insecticide. Each individual BMSB adult received 2 ul drop on the upper dorsal (just below pronotum, on the edge of scutellum) surface of the exoskeleton. The concentration of each insecticide solution was determined as equivalent of the recommended field rate of the product assuming application of 100 gal (378 l) of spray solution per acre (0.4 ha). A biological surfactant, Triton-X 100, was also added to each solution (1 ml of surfactant per 100 ml of solution). Water only plus surfactant was used as a control treatment. Treated BMSB individuals were kept in enclosed containers at 23 C and 16:8 photoperiod. The mortality of BMSB was assessed at 4, 24, 48, 72, 96 and 120 hours after the application. During each evaluation, each individual insect was scored as either live, dead, or moribund. The moribund category includes individuals unable to move forward or backward in a coordinated manner but still able to respond to touch by a camel brush by moving legs or antennae. Close to 60 individual combination of various products/rates were tested up-to-date with some products being tested multiple times at various field rates or available formulations. The results of the bioassays were made available to stakeholders throughout a series of meetings and extension publications presented throughout 2011 and 2012.