RESEARCH ON THE EPIDEMIOLOGY OF CITRUS HUANGLONGBING AND OTHER EXOTIC PATHOGENS
Project Number: 6618-22000-039-08
Specific Cooperative Agreement
Start Date: Nov 30, 2010
End Date: Aug 31, 2013
The overall objective of this research project is to conduct a series of epidemiological studies to elucidate those biological factors that are not well understood. Utilizing this new information, develop enhanced control strategies for citrus HLB. The sub objectives employed in support of the over-arching main objective are:
1. Develop new real time PCR diagnostic methodologies for Ca. Liberibacter asiaticus and Liberibacter americanus to the use for detection in both plants and insect vectors.
2. Conduct greenhouse epidemic trials using both pathogens, L. asiaticus and L. americanus, studying their spatiotemporal dynamics and interactions between the isolates.
3. Using spatiotemporal analyses monitor and estimate HLB epidemics in commercial orchards.
4. Determine acquisition and inoculation. It's a L. americanus and L. asiaticus for Diaphorina citri.
5. Examine the effect of temperature in geographic regions on HLB progress.
6. Determine the geographic distribution of the two HLB bacterial strains in the state of São Paulo Brazil.
7. Sam the progress and colonization of the two HLB strains in various citrus cultivars.
8. Determine the infectivity D. citri in commercial orchards for the two bacterial strains.
9. Based on information extracted from the above objectives, develop augmented and potentially new strategies for HLB control in commercial orchards.
Develop a coordinated, multiphased research effort on exotic citrus diseases of common concern to the US and Brazilian citrus industries with the Fundação de Estudos Agrários Luiz de Queiroz (FEALQ), Piracicaba, SP, Brazil and ARS personnel USDA, ARS, Horticultural Research Laboratory, Fort Pierce, Florida. The cooperative agreement will be developed to implement the research effort between these parties within the USA and abroad for research that cannot be performed in the U.S., or more easily facilitated outside the U.S.