2012 Annual Report
2. Detection and attraction: Develop chemical and acoustic detection and attraction systems for pest species and natural enemies: specifically develop trapping systems using floral-derived volatiles to monitor and/or eliminate pest populations and monitor dispersal of augmented parasitoids, detect acoustic signals produced by cryptic/hidden pests for targeted control, and improve detection efficiency through automation.
3. Biological control: Develop strategies for use of parasitoids and predators in IPM of insect pests through behavioral, ecological and physiological studies of their feeding, mating,dispersal and oviposition: Specifically target conservation biological control on overwintering reservoirs of migratory fall army worm and other pests; develop thelytokous strains of fruit fly parasitoids for augmentative biological control, and develop Asian citrus psyllid diets that will facilitate the mass-production of hosts for mass-rearing parasitoids for augmentative parasitoid releases and other forms of biologically-based control; and develop predictive models of pest migration that incorporate climate-change and facilitate the targeting of vulnerable populations.
To precisely target populations of hidden/cryptic pests distinctive spectral and temporal patterns of acoustic signals will be identified. Insect detectability will be optimized by constructing customized attachments or waveguides connecting the sensors to the substrates. Automated systems can be developed that remotely detect, identify, and count specific pests based on the spectral and temporal patterns of signals. An automated system for trapping C. capitata will consist of a lure and a microphone connected to a signal acquisition andanalysis system attached by cable or wireless to a computer. To develop food/host based attractants for opiine braconid fruit fly parasitoids to monitor augmentative releases and to stimulate oviposition volatiles produced by fruit fly larvae, infested fruits and nectar-sourcesare parasitoids to locate hosts, host-habitats and adult food will be identified and formulated into appropriate lures and additives. To develop floral attractants and pheromones for monitoring and/or control of adult lepidopterous pests research will concentrate on phenylacetaldehyde (PAA) plus ß-myrcene, cis-jasmone, benzyl acetate and additional candidate identified by field surveys. Lures will be first tested in flight tunnels and then in the field.
To develop thelytokous strains of fruit fly parasitoids for augmentative biological control, Wolbachia-infected parasitoid surveys will be based on the theory that asexual populations are more persistent in biologically less diverse environments. To develop improved control strategies for lepidopteran pests that recruit natural enemies, combinations of resistant crops and plants supporting natural enemies will be compared in the field using sentinel plants and a split plot arrangement of treatments. To predict the spatial and temporal shifts in infestations of migratory noctuid pests that result from climate change and to target controls, genetic markers will be used to identify source populations from which migratory pathways can be derived, and historical changes in these pathways explained with meteorological and climatic data.
Cicero, L., Sivinski, J.M., Rull, J., Aluja, M. 2011. Effect of larval host food substrate on egg load dynamics, egg size and adult female size in four species of braconid fruit fly (Diptera: Tephritidae) parasitoids. Journal of Insect Physiology. 57(11)1471:1479.
Zych, A.F., Mankin, R.W., Gillooly, J.F., Foreman, E.G. 2012. Stridulation by Jadera haematoloma (Hemiptera: Rhopalidae): Production mechanism and associated behaviors. Annals of the Entomological Society of America. 105:118-127.
Serra, C.A., Ferreira, M., Garcia, S., Santana, L., Castillo, M., Nolasco, C., Morales, P., Holler, T., Roda, A., Aluja, M.M., Sivinski, J.M. 2011. Establishment of the west indian fruit fly (Diptera: Tephritidae) parasitoid Doryctobracon areolatus (Hymenoptera: Braconidae)in the Dominican Republic. Florida Entomologist. 94(4):809-816.
Al-Dobai, S., Reitz, S.R., Sivinski, J.M. 2012. Tachinidae (Diptera) associated with flowering plants: estimating foral attractiveness. Biological Control. 61:230-239.
Ober, H.K., Degroote, L.W., Mcdonough, C.M., Mizell Iii, R.F., Mankin, R.W. 2011. Identification of an attractant for the nine-banded armadillo, dasypus novemcinctus. Wildlife Society Bulletin. 35:421-429.
Schetelig, M.A., Milano, A., Saccone, G., Handler, A.M. 2012. Male only progeny in Anastrepha suspensa by RNAi-induced sex reversion of chromosomal females. Journal of Insect Biochemistry and Molecular Biology. 42:51-57.
Fiaboe, K.K., Mankin, R.W., Roda, A.L., Kairo, M.T., Johanns, C. 2011. Pheromone-food-bait trap and acoustic surveys of Rhynchophorus Ferrugineus (Coleoptera:Curculionidae)in Curacao. Florida Entomologist. 94:766-773.
Mankin, R.W. 2012. Applications of acoustics in insect pest management. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 7:001.
Meagher Jr, R.L., Nagoshi, R.N., Stuhl, C.J. 2011. Oviposition choice of two fall armyworm (Lepidoptera: Noctuidae) host strains. Journal of Insect Behavior. 24:337-347.
Nagoshi, R.N., Meagher Jr, R.L., Brambila, J. 2011. Use of DNA barcodes to identify invasive Spodoptera species in Florida. Journal of Insect Science. 11:154.
Hay-Roe, M.M., Meagher Jr, R.L., Nagoshi, R.N. 2011. Effects of cyanogenic plants on fitness in two host strains of the fall armyworm (Spodoptera frugiperda). Journal of Chemical Ecology. 37:1314-1322.
Mankin, R.W. 2011. Recent developments in the use of acoustic sensors and signal processing tools to target early infestations of red palm weevil in agricultural environments. Florida Entomologist. 94(4):761-765.
Mukhtar, M., Rasool, K.G., Parrella, M.P., Sheikh, I., Pain, A., Lopez-Llorca, L.V., Mankin, R.W. 2011. New initiatives for managment of red palm weevil threats to historical Arabian date palms. Florida Entomologist. 94:733-736.
Stuhl, C.J., Jurado, L.C., Sivinski, J.M., Teal, P.E., Lapointe, S.L., Paranhos, B., Aluja, M. 2011. Longevity of multiple species of tephritid (Diptera) fruit fly parasitoids (Hymenoptera: Braconidae: Opiinae) provided exotic and sympatric-fruit based diets. Journal of Insect Physiology. 57:1463-1470.
Handler, A.M., O'Brochta, D.A. 2012. Transposable elements for insect transformation. In: Gilbert, L.I.,editor. Insect Biochemistry and Molecular Biology. London, United Kingdom: Academic Press. p. 90-133.
Chiu, Y.K., Mankin, R.W., Lin, C.C. 2011. Context dependent stridulatory responses of Leptogenys kitteli (Hymenoptera: Formicidae) to social, prey, and disturbance stimuli. Annals of the Entomological Society of America. 104:1012-1020.
Mankin, R.W., Hagstrum, D.W. 2011. Acoustic monitoring of insects. In: Hagstrum, D.W., Phillips, T.W., Cuperus,G., editors. Stored Product Production. Manhattan, Kansas: Kansas State University Press. p. 1-7.
Davies, A.P., Zalucki, M.P., Pufke, U.S. 2011. Spatio-temporal variation in Helicoverpa egg parasitism by Trichogramma in a tropical Bt-transgenic cotton landscape. Agricultural and Forest Entomology. 13:247-258.
Davies, A.P., Carr, C.M., Scholz, B.G., Zalucki, M.P. 2011. Using Trichogramma Westwood (Hymenoptera: Trichogrammatidae) for insect pest biological control in cotton crops: an Australian perspective. Australian Journal of Entomology. 50:424-440.
Nagoshi, R.N., Murua, G.M., Hay-Roe, M.M., Juarez, L.M., Willink, E., Meagher Jr, R.L. 2012. Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina. Journal of Economic Entomology. 105(2):418-428.
Harris, C.M., Ruberson, J.R., Meagher Jr, R.L., Tumlinson, J.H. 2012. Host suitability affects odor association in Cotesia marginiventris: implications in generalist parasitoid host-finding. Journal of Chemical Ecology. 38(4):340-347.
Cicero, L., Sivinski, J.M., Aluja, M. 2012. Effect of host diet and adult parasitoid diet on egg load dynamics and egg size of braconid parasitoids attacking Anastrepha ludens. Physiological Entomology. 37(2):177-184.
Nagoshi, R.N. 2012. Improvements in the identification of strains facilitate population studies of fall armyworm subgroups. Annals of the Entomological Society of America. 105(2):351-358.
Gruters Thomas, J.M., Shirk, P.D., Shapiro, J.P., Allan, S.A. 2012. Mass-rearing of a tropical minute pirate bug, Orius Pumilio (Hemiptera:Anthocoridae). Florida Entomologist. 95(1):199-201.
Arthurs, S.P., Tofangsazi, N., Meagher Jr, R.L., Cherry, R. 2012. Attraction of Plecia nearctica (dipter:bibionidae) to floral lures containing phenylacetaldehyde. Florida Entomologist. 95(1):199-201.
Ramirez-Romero, R., Sivinski, J.M., Copeland, C.S., Aluja, M. 2012. Are individuals stemming from thelytokous and arrhenotokous populations equally adept as biocontrol agents? Orientation and host searching behavior of a fruit fly parasitoid. Biocontrol. 57:427-440.
Long, D., Zhao, A., Chen, X., Zhang, Y., Lu, W., Guo, Q., Handler, A.M. 2012. FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. PLoS One. 7(6):e40150. doi: 10.1371/journal.pone.00401.