Location: Food and Feed Safety Research
Project Number: 6435-42000-022-00
Start Date: Dec 28, 2010
End Date: Dec 27, 2015
Aflatoxins (AFs) are polyketide-derived, toxic and carcinogenic secondary metabolites produced mainly by Aspergillus (A.) flavus on cotton, corn, peanuts, and tree nuts. One of the main strategies for prevention of AF contamination in these crops involves introducing a non-aflatoxigenic competitor Aspergillus to the soil of the plants during the growing season. Recent work revealed that A. flavus has a complicated evolutionary history that includes a history of recombination. It is the purpose of this proposal to better understand A. flavus population dynamics in agricultural environments in order to more safely use biocontrol as a strategy to reduce crop contamination with AFs. To do this we will develop new competitor strains with improved properties for tracking their dispersal after introduction onto crops and with improved ability to over-winter in order to decrease the need for annual reapplication. Optimal candidates should be unable to produce the neurotoxin, cyclopiazonic acid, without altering their competitive ability. The potential for restoration of both AF- and CPA-producing ability of the atoxigenic strain in the laboratory or the field will be assessed. We will determine if the ability to produce hydrolases by the biocontrol strain is important for its competitive ability. With these studies, we expect to be able to either develop new biocontrol strains or improve the design of currently used biocontrol strains to reduce preharvest AF contamination. We also expect to provide additional insight into the evolution of diversity within A. flavus.