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United States Department of Agriculture

Agricultural Research Service

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Research Project: Development of Rapid Detection Methods Using Nanobiosensors for Salmonella and Vaccine Against Se and St for Poultry

Location: Quality and Safety Assessment Research Unit

Project Number: 6040-42000-041-03
Project Type: Trust

Start Date: Oct 01, 2010
End Date: Dec 31, 2014

Objective:
To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: 1) determine SERS characteristics of Salmonella; 2) develop chemometric models to differentiate Salmonella from other bacteria; 3) classify different Salmonella strains; and 4) evaluate detection limits of nanobiosensor.

Approach:
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: 1) Design, fabricate SERS substrate to optimize for lower detection limits; 2) Salmonella sample preparation; 3) SERS measurement; 4) Test and validate classification models for different serotypes; 4) Test detectability from different background flora.

Last Modified: 10/20/2014
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