DEVELOPMENT OF RAPID DETECTION METHODS USING NANOBIOSENSORS FOR SALMONELLA AND VACCINE AGAINST SE AND ST FOR POULTRY
Quality and Safety Assessment Research Unit
Project Number: 6612-42000-041-03
Start Date: Oct 01, 2010
End Date: Dec 31, 2014
To develop a nanobiosensor to detect Salmonella using surface-enhanced Raman scattering (SERS) technique: 1) determine SERS characteristics of Salmonella; 2) develop chemometric models to differentiate Salmonella from other bacteria; 3) classify different Salmonella strains; and 4) evaluate detection limits of nanobiosensor.
We will develop methodologies to test and validate the SERS techniques. A series of experiments will be conducted based on the following methods: 1) Design, fabricate SERS substrate to optimize for lower detection limits; 2) Salmonella sample preparation; 3) SERS measurement; 4) Test and validate classification models for different serotypes; 4) Test detectability from different background flora.