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United States Department of Agriculture

Agricultural Research Service

Research Project: Evaluation of Newcastle Disease Virus Vaccines Against Circulating Viruses

Location: Exotic and Emerging Avian Viral Diseases Research Unit

Project Number: 6612-32000-064-06
Project Type: Trust

Start Date: Mar 01, 2011
End Date: Feb 28, 2014

Objective:
1. Determine vaccine efficacy following vaccination with inactivated Newcastle disease virus antigen vaccine against highly pathogenic Newcastle disease virus challenge. 2. Determine seroconversion against Newcastle disease virus (NDV) following vaccination of 4 week old specific pathogen free (SPF) chickens with inactivated NDV vaccines.

Approach:
1. Viruses representative of circulating genotypes will be grown to high titers in eggs. 2. Amount of antigen to be used will be compared and standardized using a monoclonal antibody that recognizes a highly conserved epitope present in all viruses. 3. The antigen will be denatured using standard techniques and injected in 4 week SPF chickens to determine immune response and protection against NDV challenge. 4. Efficacy will be determined using high pathogenicity Newcastle disease virus in 4 week old SPF chicken with measurement of protection being prevention of morbidity and mortality, reduction in number of infected birds, and a decrease in the NDV-shed from respiratory and alimentary tracts. 5. Two antigens from genotype VI and VII will be compared to a mock vaccinated and to a standard vaccine based on a lentogenic virus and three different challenge viruses will be used (4x3 a total of 12 cages).

Last Modified: 9/1/2014
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