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United States Department of Agriculture

Agricultural Research Service

Research Project: Strengthening Public Corn Breeding to Ensure That Organic Farmers Have Access to Elite Cultivars:quality Analysis

Location: Corn Insects and Crop Genetics Research

Project Number: 3625-21000-063-07
Project Type: General Cooperative Agreement

Start Date: May 19, 2011
End Date: Aug 31, 2014

Objective:
1) Update calibrations for grain composition and amino acids, 2) update calibrations for and standardize five cooperator instruments, and 3) train and support cooperators in quality control and lab operations.

Approach:
The calibration samples will be provided by collaborating plant breeders. We estimate that 400 total samples per year will be screened for possible use as calibration samples. The GCL NIRS values will be compared to any original values from the breeder cooperator, which will provide an estimate of testing variability (standard deviation). GQL will scan the samples, and will collect the spectral data, sample information, and measured values. Samples for reference analysis (n=100; 50, 50, 50 per year, respectively) will be sent to the University of Missouri. Calibrations will be validated annually according to AACC Method 39-00, and updated if needed by April 30 of the respective marketing year. Updates are done by inclusion of the new samples in the calibration data set of the machine brand being evaluated. Calibrations/instrument setup for the following fall harvest will be done by the start of harvest. Temperature stability is introduced/verified by testing the 10-annual temperature samples at four temperatures (-5, 5, 25, and 45oC), using the accuracy criteria of AACC Method 39-00 to determine if additional temperature stability is needed. Standardization/traceability to the source of calibrations is necessary for accurate data from users’ instruments. Five cooperator instruments will be standardized against the calibration master units at GQL and against the wet chemistry references from University of Missouri, using 40 corn samples that have at least 3 replicate reference values for each factor. Annual verification of the standardization will be done with data from the cooperator samples. A new standardization with the GQL master samples will be needed if the calibrations are changed during the project period. The presence of these verified cooperator units will form the basis for a network of testing sites for cooperators specializing in marketing or utilizing the kinds of organic grains we are breeding. Project cooperators will determine the ongoing operating structure of this network, if created. Initial training, then annual support will be held with cooperators (at the time of the annual project meeting), with the intent to have cooperators self-sufficient in quality control and lab operations before the end of the project.

Last Modified: 8/1/2014
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