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United States Department of Agriculture

Agricultural Research Service

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Research Project: FOOT-AND-MOUTH DISEASE MONOCLONAL ANTIBODIES PRODUCTION IN CAMELS

Location: Foreign Animal Disease Research

Project Number: 1940-32000-057-18
Project Type: Specific Cooperative Agreement

Start Date: May 01, 2011
End Date: Sep 30, 2013

Objective:
The goal of this collaborative research project is to produce camel monoclonal antibodies (cMAb) against Foot-and-Mouth Disease Virus (FMDV) non-structural proteins (NSP) and virus particles (SP). USDA, ARS and Ben Gurion University scientists will design a reagent for viruses that will target three relevant FMDV serotyes including O, A and the South African Territories (SAT) types of Foot-and-Mouth Disease Virus (FMDV). An additional objective includes an attempt to assemble diagnostics assays using selected virus-specific cMAb developed in this project. Currently, there are no effective “in house” and affordable surveillance diagnostic test widely available in sub-Saharan Africa.

Approach:
Virus specific camel monoclonal antibodies (cMAbs) in camelids directed against the Foot-and-Mouth Disease Virus (FMDV) structural proteins (SP) and non-structural proteins (NSPs) will be produced. Using an eukaryotic expression system tailored to the FMDV proteins, reactive hybridoma clones to the capsid, the 3D and 3ABC proteins will be screened and selected. Additionally, efforts will be aimed at the development of suitable and easy to deploy DIVA (differentiation between infected and vaccinated animals) (DIVA) tests utilizing the camel MAbs in a test platform. The formulated test/s will be bench standardized to assess the rapid and accurate detection of anti-NSP and FMDV detection. The specificity, stability and affinity characteristics of camel MAbs make them excellent choices in biosensor applications and they will potentially be more stable reagents when applied in a validated test.

Last Modified: 9/2/2014
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