Location: Foreign Animal Disease Research
Project Number: 1940-32000-056-11
Start Date: Sep 01, 2011
End Date: Mar 31, 2014
To fulfill objective 1, a set of genetically modified ASFV deletion mutants will be constructed from virulent wild-type viruses. Virulence of mutant viruses will be determined in swine relative to virulence of parental wild-type viruses. To fulfill objective 2, infections with wild-type and mutant ASFV will be established in vivo and in relevant cells of swine origin in vitro. Persistently and acutely infected cells and recovered viruses will be analyzed by a combination of techniques including microscopy, fluorescence confocal microscopy, qPCR, sequencing and sequencing analysis. Isolated viruses will be characterized in vitro and in vivo relative to wild-type viruses. Identification and characterization of host and viral mediating disease will be an important goal in this project. Overall experimental manipulation of identified host-virus interactions may be used for developing novel tools for controlling virus infection in vivo.