Location: Hard Winter Wheat Genetics Research Unit
Project Number: 5430-21000-010-14
Start Date: Sep 15, 2011
End Date: Sep 14, 2016
Association mapping populations have been constructed and are now being analyzed. For genotyping, we will use conventional SSRs, STS, and SNP markers and a genotyping-by-sequencing (GBS) approach suitable for the large complex genomes of wheat. Genotyping-by-sequencing uses restriction enzymes to produce a reduced representation of the genome that is then multiplex sequenced on the Illumina platform using DNA barcode adapters. We are able to identify tens of thousands of SNPs in each sample and use this information for genetic mapping and breeding. We will use the GBS data to identify SNP markers closely linked with the resistance genes while making background selection for the recurrent parent genotype. This marker assisted back-crossing approach will 1) identify molecular markers linked to traits of interest and 2) rapidly recover the elite wheat background for using these genes in breeding.